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Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration

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2021
Authors
Ćupić-Miladinović, Dejana
Prevendar-Crnić, Andreja
Peković, Sanja
Dacić, Sanja
Ivanović, Saša
Santibanez, Juan
Ćupić, Vitomir
Borozan, Nevena
Antonijević-Miljaković, Evica
Borozan, Sunčica
Article (Published version)
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Abstract
Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione5-trans...ferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.

Keywords:
Chlorpyrifos / Vitamin B1 / Japanese quail / Inflammation / Oxidative stress / Brain
Source:
Chemico-Biological Interactions, 2021, 333, 109312-
Publisher:
  • Elsevier
Funding / projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200143 (University of Belgrade, Faculty of Veterinary Medicine) (RS-200143)

DOI: 10.1016/j.cbi.2020.109312

ISSN: 0009-2797

WoS: 000600832900017

Scopus: 2-s2.0-85096159138
[ Google Scholar ]
3
URI
http://rimi.imi.bg.ac.rs/handle/123456789/1073
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Ćupić-Miladinović, Dejana
AU  - Prevendar-Crnić, Andreja
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Ivanović, Saša
AU  - Santibanez, Juan
AU  - Ćupić, Vitomir
AU  - Borozan, Nevena
AU  - Antonijević-Miljaković, Evica
AU  - Borozan, Sunčica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1073
AB  - Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione5-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration
SP  - 109312
VL  - 333
DO  - 10.1016/j.cbi.2020.109312
ER  - 
@article{
author = "Ćupić-Miladinović, Dejana and Prevendar-Crnić, Andreja and Peković, Sanja and Dacić, Sanja and Ivanović, Saša and Santibanez, Juan and Ćupić, Vitomir and Borozan, Nevena and Antonijević-Miljaković, Evica and Borozan, Sunčica",
year = "2021",
abstract = "Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione5-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration",
pages = "109312",
volume = "333",
doi = "10.1016/j.cbi.2020.109312"
}
Ćupić-Miladinović, D., Prevendar-Crnić, A., Peković, S., Dacić, S., Ivanović, S., Santibanez, J., Ćupić, V., Borozan, N., Antonijević-Miljaković, E.,& Borozan, S.. (2021). Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration. in Chemico-Biological Interactions
Elsevier., 333, 109312.
https://doi.org/10.1016/j.cbi.2020.109312
conv_4937
Ćupić-Miladinović D, Prevendar-Crnić A, Peković S, Dacić S, Ivanović S, Santibanez J, Ćupić V, Borozan N, Antonijević-Miljaković E, Borozan S. Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration. in Chemico-Biological Interactions. 2021;333:109312.
doi:10.1016/j.cbi.2020.109312
conv_4937 .
Ćupić-Miladinović, Dejana, Prevendar-Crnić, Andreja, Peković, Sanja, Dacić, Sanja, Ivanović, Saša, Santibanez, Juan, Ćupić, Vitomir, Borozan, Nevena, Antonijević-Miljaković, Evica, Borozan, Sunčica, "Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration" in Chemico-Biological Interactions, 333 (2021):109312,
https://doi.org/10.1016/j.cbi.2020.109312 .,
conv_4937 .

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