Приказ основних података о документу

dc.creatorKovačević, Sanjin
dc.creatorIvanov, Milan
dc.creatorMiloradović, Zoran
dc.creatorBrkić, Predrag
dc.creatorVajić, Una Jovana
dc.creatorŽivotić, Maja
dc.creatorMihailović-Stanojević, Nevena
dc.creatorJovović, Đurđica
dc.creatorKaranović, Danijela
dc.creatorJeremić, Rada
dc.creatorNešović-Ostojić, Jelena
dc.date.accessioned2021-04-20T13:10:45Z
dc.date.available2021-04-20T13:10:45Z
dc.date.issued2020
dc.identifier.issn1932-6203
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/1066
dc.description.abstractRenal ischemia/reperfusion injury is a common cause of acute kidney injury (AKI) and hypertension might contribute to the increased incidence of AKI. The purpose of this study was to investigate the effects of single and combined hyperbaric oxygen (HBO) preconditioning and NADPH oxidase inhibition on oxidative stress, kidney function and structure in spontaneously hypertensive rats (SHR) after renal ischemia reperfusion injury. HBO preconditioning was performed by exposing to pure oxygen (2.026 bar) twice a day for two consecutive days for 60 minutes, and 24h before AKI induction. For AKI induction, the right kidney was removed and ischemia was performed by clamping the left renal artery for 45 minutes. NADPH oxidase inhibition was induced by apocynin (40 mg/kg b.m., intravenously) 5 minutes before reperfusion. AKI significantly increased renal vascular resistance and reduced renal blood flow, which were significantly improved after apocynin treatment. Also, HBO preconditioning, with or without apocynin treatment showed improvement on renal hemodynamics. AKI significantly increased plasma creatinine, urea, phosphate levels and lipid peroxidation in plasma. Remarkable improvement, with decrease in creatinine, urea and phosphate levels was observed in all treated groups. HBO preconditioning, solitary or with apocynin treatment decreased lipid peroxidation in plasma caused by AKI induction. Also, combined with apocynin, it increased catalase activity and solitary, glutathione reductase enzyme activity in erythrocytes. While AKI induction significantly increased plasma KIM- 1 levels, HBO preconditioning, solitary or with apocynin decreased its levels. Considering renal morphology, significant morphological alterations present after AKI induction were significantly improved in all treated groups with reduced tubular dilatation, tubular necrosis in the cortico-medullary zone and PAS positive cast formation. Our results reveal that NADPH oxidase inhibition and hyperbaric oxygen preconditioning, with or without NADPH oxidase inhibition may have beneficial effects, but their protective role should be evaluated in further studies.en
dc.publisherPublic Library Science, San Francisco
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175096/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePLoS One
dc.titleHyperbaric oxygen preconditioning and the role of NADPH oxidase inhibition in postischemic acute kidney injury induced in spontaneously hypertensive ratsen
dc.typearticle
dc.rights.licenseBY
dc.citation.issue1
dc.citation.other15(1): -
dc.citation.rankM22~
dc.citation.spagee0226974
dc.citation.volume15
dc.identifier.doi10.1371/journal.pone.0226974
dc.identifier.fulltexthttp://rimi.imi.bg.ac.rs/bitstream/id/53/1063.pdf
dc.identifier.pmid31914135
dc.identifier.scopus2-s2.0-85077731394
dc.identifier.wos000534337700023
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу