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Hyperbaric oxygen preconditioning and the role of NADPH oxidase inhibition in postischemic acute kidney injury induced in spontaneously hypertensive rats

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2020
1063.pdf (2.505Mb)
Authors
Kovačević, Sanjin
Ivanov, Milan
Miloradović, Zoran
Brkić, Predrag
Vajić, Una-Jovana
Životić, Maja
Mihailović-Stanojević, Nevena
Jovović, Đurđica
Karanović, Danijela
Jeremić, Rada
Nešović-Ostojić, Jelena
Article (Published version)
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Abstract
Renal ischemia/reperfusion injury is a common cause of acute kidney injury (AKI) and hypertension might contribute to the increased incidence of AKI. The purpose of this study was to investigate the effects of single and combined hyperbaric oxygen (HBO) preconditioning and NADPH oxidase inhibition on oxidative stress, kidney function and structure in spontaneously hypertensive rats (SHR) after renal ischemia reperfusion injury. HBO preconditioning was performed by exposing to pure oxygen (2.026 bar) twice a day for two consecutive days for 60 minutes, and 24h before AKI induction. For AKI induction, the right kidney was removed and ischemia was performed by clamping the left renal artery for 45 minutes. NADPH oxidase inhibition was induced by apocynin (40 mg/kg b.m., intravenously) 5 minutes before reperfusion. AKI significantly increased renal vascular resistance and reduced renal blood flow, which were significantly improved after apocynin treatment. Also, HBO preconditioning, with o...r without apocynin treatment showed improvement on renal hemodynamics. AKI significantly increased plasma creatinine, urea, phosphate levels and lipid peroxidation in plasma. Remarkable improvement, with decrease in creatinine, urea and phosphate levels was observed in all treated groups. HBO preconditioning, solitary or with apocynin treatment decreased lipid peroxidation in plasma caused by AKI induction. Also, combined with apocynin, it increased catalase activity and solitary, glutathione reductase enzyme activity in erythrocytes. While AKI induction significantly increased plasma KIM- 1 levels, HBO preconditioning, solitary or with apocynin decreased its levels. Considering renal morphology, significant morphological alterations present after AKI induction were significantly improved in all treated groups with reduced tubular dilatation, tubular necrosis in the cortico-medullary zone and PAS positive cast formation. Our results reveal that NADPH oxidase inhibition and hyperbaric oxygen preconditioning, with or without NADPH oxidase inhibition may have beneficial effects, but their protective role should be evaluated in further studies.

Source:
PLoS One, 2020, 15, 1, e0226974-
Publisher:
  • Public Library Science, San Francisco
Funding / projects:
  • Investigation of antihypertensiv? and renoprotectiv? potential of natural and synthetic compounds in the experimental models of cardiovascular and renal diseases (RS-175096)

DOI: 10.1371/journal.pone.0226974

ISSN: 1932-6203

PubMed: 31914135

WoS: 000534337700023

Scopus: 2-s2.0-85077731394
[ Google Scholar ]
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5
URI
http://rimi.imi.bg.ac.rs/handle/123456789/1066
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Miloradović, Zoran
AU  - Brkić, Predrag
AU  - Vajić, Una-Jovana
AU  - Životić, Maja
AU  - Mihailović-Stanojević, Nevena
AU  - Jovović, Đurđica
AU  - Karanović, Danijela
AU  - Jeremić, Rada
AU  - Nešović-Ostojić, Jelena
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1066
AB  - Renal ischemia/reperfusion injury is a common cause of acute kidney injury (AKI) and hypertension might contribute to the increased incidence of AKI. The purpose of this study was to investigate the effects of single and combined hyperbaric oxygen (HBO) preconditioning and NADPH oxidase inhibition on oxidative stress, kidney function and structure in spontaneously hypertensive rats (SHR) after renal ischemia reperfusion injury. HBO preconditioning was performed by exposing to pure oxygen (2.026 bar) twice a day for two consecutive days for 60 minutes, and 24h before AKI induction. For AKI induction, the right kidney was removed and ischemia was performed by clamping the left renal artery for 45 minutes. NADPH oxidase inhibition was induced by apocynin (40 mg/kg b.m., intravenously) 5 minutes before reperfusion. AKI significantly increased renal vascular resistance and reduced renal blood flow, which were significantly improved after apocynin treatment. Also, HBO preconditioning, with or without apocynin treatment showed improvement on renal hemodynamics. AKI significantly increased plasma creatinine, urea, phosphate levels and lipid peroxidation in plasma. Remarkable improvement, with decrease in creatinine, urea and phosphate levels was observed in all treated groups. HBO preconditioning, solitary or with apocynin treatment decreased lipid peroxidation in plasma caused by AKI induction. Also, combined with apocynin, it increased catalase activity and solitary, glutathione reductase enzyme activity in erythrocytes. While AKI induction significantly increased plasma KIM- 1 levels, HBO preconditioning, solitary or with apocynin decreased its levels. Considering renal morphology, significant morphological alterations present after AKI induction were significantly improved in all treated groups with reduced tubular dilatation, tubular necrosis in the cortico-medullary zone and PAS positive cast formation. Our results reveal that NADPH oxidase inhibition and hyperbaric oxygen preconditioning, with or without NADPH oxidase inhibition may have beneficial effects, but their protective role should be evaluated in further studies.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Hyperbaric oxygen preconditioning and the role of NADPH oxidase inhibition in postischemic acute kidney injury induced in spontaneously hypertensive rats
IS  - 1
SP  - e0226974
VL  - 15
DO  - 10.1371/journal.pone.0226974
ER  - 
@article{
author = "Kovačević, Sanjin and Ivanov, Milan and Miloradović, Zoran and Brkić, Predrag and Vajić, Una-Jovana and Životić, Maja and Mihailović-Stanojević, Nevena and Jovović, Đurđica and Karanović, Danijela and Jeremić, Rada and Nešović-Ostojić, Jelena",
year = "2020",
abstract = "Renal ischemia/reperfusion injury is a common cause of acute kidney injury (AKI) and hypertension might contribute to the increased incidence of AKI. The purpose of this study was to investigate the effects of single and combined hyperbaric oxygen (HBO) preconditioning and NADPH oxidase inhibition on oxidative stress, kidney function and structure in spontaneously hypertensive rats (SHR) after renal ischemia reperfusion injury. HBO preconditioning was performed by exposing to pure oxygen (2.026 bar) twice a day for two consecutive days for 60 minutes, and 24h before AKI induction. For AKI induction, the right kidney was removed and ischemia was performed by clamping the left renal artery for 45 minutes. NADPH oxidase inhibition was induced by apocynin (40 mg/kg b.m., intravenously) 5 minutes before reperfusion. AKI significantly increased renal vascular resistance and reduced renal blood flow, which were significantly improved after apocynin treatment. Also, HBO preconditioning, with or without apocynin treatment showed improvement on renal hemodynamics. AKI significantly increased plasma creatinine, urea, phosphate levels and lipid peroxidation in plasma. Remarkable improvement, with decrease in creatinine, urea and phosphate levels was observed in all treated groups. HBO preconditioning, solitary or with apocynin treatment decreased lipid peroxidation in plasma caused by AKI induction. Also, combined with apocynin, it increased catalase activity and solitary, glutathione reductase enzyme activity in erythrocytes. While AKI induction significantly increased plasma KIM- 1 levels, HBO preconditioning, solitary or with apocynin decreased its levels. Considering renal morphology, significant morphological alterations present after AKI induction were significantly improved in all treated groups with reduced tubular dilatation, tubular necrosis in the cortico-medullary zone and PAS positive cast formation. Our results reveal that NADPH oxidase inhibition and hyperbaric oxygen preconditioning, with or without NADPH oxidase inhibition may have beneficial effects, but their protective role should be evaluated in further studies.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Hyperbaric oxygen preconditioning and the role of NADPH oxidase inhibition in postischemic acute kidney injury induced in spontaneously hypertensive rats",
number = "1",
pages = "e0226974",
volume = "15",
doi = "10.1371/journal.pone.0226974"
}
Kovačević, S., Ivanov, M., Miloradović, Z., Brkić, P., Vajić, U., Životić, M., Mihailović-Stanojević, N., Jovović, Đ., Karanović, D., Jeremić, R.,& Nešović-Ostojić, J.. (2020). Hyperbaric oxygen preconditioning and the role of NADPH oxidase inhibition in postischemic acute kidney injury induced in spontaneously hypertensive rats. in PLoS One
Public Library Science, San Francisco., 15(1), e0226974.
https://doi.org/10.1371/journal.pone.0226974
conv_4789
Kovačević S, Ivanov M, Miloradović Z, Brkić P, Vajić U, Životić M, Mihailović-Stanojević N, Jovović Đ, Karanović D, Jeremić R, Nešović-Ostojić J. Hyperbaric oxygen preconditioning and the role of NADPH oxidase inhibition in postischemic acute kidney injury induced in spontaneously hypertensive rats. in PLoS One. 2020;15(1):e0226974.
doi:10.1371/journal.pone.0226974
conv_4789 .
Kovačević, Sanjin, Ivanov, Milan, Miloradović, Zoran, Brkić, Predrag, Vajić, Una-Jovana, Životić, Maja, Mihailović-Stanojević, Nevena, Jovović, Đurđica, Karanović, Danijela, Jeremić, Rada, Nešović-Ostojić, Jelena, "Hyperbaric oxygen preconditioning and the role of NADPH oxidase inhibition in postischemic acute kidney injury induced in spontaneously hypertensive rats" in PLoS One, 15, no. 1 (2020):e0226974,
https://doi.org/10.1371/journal.pone.0226974 .,
conv_4789 .

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