Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study
2020
Preuzimanje 🢃
Autori
Lazarević, MilošMilošević, Maja
Jelovac, Drago
Milenković, Sanja
Tepavčević, Zvezdana
Baldan, Federica
Subotički, Tijana
Toljić, Bosko
Trisić, Dijana
Dragović, Miroslav
Damante, Giuseppe
Milasin, Jelena
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associated markers, including vimentin, alpha -smooth muscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were note...d with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics.
Ključne reči:
oral cancer / epithelial to mesenchymal transition / surgical margins / gene expression / cell proliferation and migrationIzvor:
Oncology Letters, 2020, 19, 6, 3743-3750Izdavač:
- Spandidos Publ Ltd, Athens
Finansiranje / projekti:
- Ministry of Foreign Affairs of Italy [PGR02954]
- Genetička kontrola i molekularni mehanizmi u malignim, inflamatornim i razvojnim patologijama orofacijalne regije (RS-MESTD-Basic Research (BR or ON)-175075)
- Ćelijske i molekulske osnove malignih i kardiovaskularnih oboljenja-kliničke implikacije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
DOI: 10.3892/ol.2020.11494
ISSN: 1792-1074
PubMed: 32382327
WoS: 000535780700015
Scopus: 2-s2.0-85083633185
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Lazarević, Miloš AU - Milošević, Maja AU - Jelovac, Drago AU - Milenković, Sanja AU - Tepavčević, Zvezdana AU - Baldan, Federica AU - Subotički, Tijana AU - Toljić, Bosko AU - Trisić, Dijana AU - Dragović, Miroslav AU - Damante, Giuseppe AU - Milasin, Jelena PY - 2020 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1064 AB - Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associated markers, including vimentin, alpha -smooth muscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics. PB - Spandidos Publ Ltd, Athens T2 - Oncology Letters T1 - Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study EP - 3750 IS - 6 SP - 3743 VL - 19 DO - 10.3892/ol.2020.11494 ER -
@article{ author = "Lazarević, Miloš and Milošević, Maja and Jelovac, Drago and Milenković, Sanja and Tepavčević, Zvezdana and Baldan, Federica and Subotički, Tijana and Toljić, Bosko and Trisić, Dijana and Dragović, Miroslav and Damante, Giuseppe and Milasin, Jelena", year = "2020", abstract = "Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associated markers, including vimentin, alpha -smooth muscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics.", publisher = "Spandidos Publ Ltd, Athens", journal = "Oncology Letters", title = "Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study", pages = "3750-3743", number = "6", volume = "19", doi = "10.3892/ol.2020.11494" }
Lazarević, M., Milošević, M., Jelovac, D., Milenković, S., Tepavčević, Z., Baldan, F., Subotički, T., Toljić, B., Trisić, D., Dragović, M., Damante, G.,& Milasin, J.. (2020). Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study. in Oncology Letters Spandidos Publ Ltd, Athens., 19(6), 3743-3750. https://doi.org/10.3892/ol.2020.11494
Lazarević M, Milošević M, Jelovac D, Milenković S, Tepavčević Z, Baldan F, Subotički T, Toljić B, Trisić D, Dragović M, Damante G, Milasin J. Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study. in Oncology Letters. 2020;19(6):3743-3750. doi:10.3892/ol.2020.11494 .
Lazarević, Miloš, Milošević, Maja, Jelovac, Drago, Milenković, Sanja, Tepavčević, Zvezdana, Baldan, Federica, Subotički, Tijana, Toljić, Bosko, Trisić, Dijana, Dragović, Miroslav, Damante, Giuseppe, Milasin, Jelena, "Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study" in Oncology Letters, 19, no. 6 (2020):3743-3750, https://doi.org/10.3892/ol.2020.11494 . .