In this paper, two strategies for in vitro tissue cultivation are presented Microencapsulation is aimed for transplantation of functional tissues encapsulated in semi-permeable membranes, which provide efficient transport of nutrients, gases and small molecules necessary for tissue survival and normal function, and, in the same time, tissue protection from immunological response. Pancreatic islets were encapsulated in poly-L-ornitine - alginate microcapsules by electrostatic droplet generation technique for potential treatment of diabetes. Another approach to tissue cultivation is in vitro regeneration of functional tissue equivalents based on autologous cell biodegradable polymer scaffolds and bioreactor cultivation. Under optimal in vitro conditions (three-dimensional, biodegradable polymer scaffolds dynamic laminar flow in rotating bioreactors) biochemical composition morphology and biomechanical properties of engineered cartilage tissue approached those of the native tissue.
U ovom radu su prikazana dva pravca razvoja kultura tkiva za potencijalnu primenu u medicini. Mikroenkapsulacija je usmerena na transplantaciju funkcionalnih tkiva zaštićenih polupropustljivom membranom koja treba da obezbedi efikasnu razmenu materija neophodnih za održavanje viabilnosti i funkcije tkiva, i istovremenu zaštitu od imunološkog odgovora organizma. Kao model sistem, ćelije pankreasa su enkapsulirane primenom tehnike elektrostatičke ekstruzije u mikrokapsule od alginata i poli-L-ornitina za potencijalnu primenu u lečenju dijabetesa. Drugi pravac istraživanja predstavlja in vitro kultivaciju funkcionalnih tkiva baziranu na autolognim ćelijama, biodegradabilnim polimernim nosačima i bioreaktorskoj kultivaciji. Optimizacijom karakteristika polimernih nosača ćelija, uslova kultivacije tipa i režima rada bioreaktora dobijeno je kultivisano tkivo hrskavice koje se po biohemijskim, strukturnim, morfološkim i biomehaničkim karakteristikama približava prirodnom tkivu.
TY - JOUR
AU - Obradović, Bojana
AU - Bugarski, Branko
AU - Bugarski, Diana
AU - Vunjak-Novaković, Gordana
PY - 2004
UR - http://rimi.imi.bg.ac.rs/handle/123456789/106
AB - In this paper, two strategies for in vitro tissue cultivation are presented Microencapsulation is aimed for transplantation of functional tissues encapsulated in semi-permeable membranes, which provide efficient transport of nutrients, gases and small molecules necessary for tissue survival and normal function, and, in the same time, tissue protection from immunological response. Pancreatic islets were encapsulated in poly-L-ornitine - alginate microcapsules by electrostatic droplet generation technique for potential treatment of diabetes. Another approach to tissue cultivation is in vitro regeneration of functional tissue equivalents based on autologous cell biodegradable polymer scaffolds and bioreactor cultivation. Under optimal in vitro conditions (three-dimensional, biodegradable polymer scaffolds dynamic laminar flow in rotating bioreactors) biochemical composition morphology and biomechanical properties of engineered cartilage tissue approached those of the native tissue.
AB - U ovom radu su prikazana dva pravca razvoja kultura tkiva za potencijalnu primenu u medicini. Mikroenkapsulacija je usmerena na transplantaciju funkcionalnih tkiva zaštićenih polupropustljivom membranom koja treba da obezbedi efikasnu razmenu materija neophodnih za održavanje viabilnosti i funkcije tkiva, i istovremenu zaštitu od imunološkog odgovora organizma. Kao model sistem, ćelije pankreasa su enkapsulirane primenom tehnike elektrostatičke ekstruzije u mikrokapsule od alginata i poli-L-ornitina za potencijalnu primenu u lečenju dijabetesa. Drugi pravac istraživanja predstavlja in vitro kultivaciju funkcionalnih tkiva baziranu na autolognim ćelijama, biodegradabilnim polimernim nosačima i bioreaktorskoj kultivaciji. Optimizacijom karakteristika polimernih nosača ćelija, uslova kultivacije tipa i režima rada bioreaktora dobijeno je kultivisano tkivo hrskavice koje se po biohemijskim, strukturnim, morfološkim i biomehaničkim karakteristikama približava prirodnom tkivu.
PB - Institut bezbednosti, Beograd
T2 - Nauka, tehnika, bezbednost
T1 - In vitro tissue cultures: Microencapsulation and bioreactor cultivation
T1 - In vitro kulture tkiva - mikroenkapsulacija i bioreaktorska kultivacija
EP - 68
IS - 2
SP - 59
VL - 14
UR - conv_1082
ER -
@article{
author = "Obradović, Bojana and Bugarski, Branko and Bugarski, Diana and Vunjak-Novaković, Gordana",
year = "2004",
abstract = "In this paper, two strategies for in vitro tissue cultivation are presented Microencapsulation is aimed for transplantation of functional tissues encapsulated in semi-permeable membranes, which provide efficient transport of nutrients, gases and small molecules necessary for tissue survival and normal function, and, in the same time, tissue protection from immunological response. Pancreatic islets were encapsulated in poly-L-ornitine - alginate microcapsules by electrostatic droplet generation technique for potential treatment of diabetes. Another approach to tissue cultivation is in vitro regeneration of functional tissue equivalents based on autologous cell biodegradable polymer scaffolds and bioreactor cultivation. Under optimal in vitro conditions (three-dimensional, biodegradable polymer scaffolds dynamic laminar flow in rotating bioreactors) biochemical composition morphology and biomechanical properties of engineered cartilage tissue approached those of the native tissue., U ovom radu su prikazana dva pravca razvoja kultura tkiva za potencijalnu primenu u medicini. Mikroenkapsulacija je usmerena na transplantaciju funkcionalnih tkiva zaštićenih polupropustljivom membranom koja treba da obezbedi efikasnu razmenu materija neophodnih za održavanje viabilnosti i funkcije tkiva, i istovremenu zaštitu od imunološkog odgovora organizma. Kao model sistem, ćelije pankreasa su enkapsulirane primenom tehnike elektrostatičke ekstruzije u mikrokapsule od alginata i poli-L-ornitina za potencijalnu primenu u lečenju dijabetesa. Drugi pravac istraživanja predstavlja in vitro kultivaciju funkcionalnih tkiva baziranu na autolognim ćelijama, biodegradabilnim polimernim nosačima i bioreaktorskoj kultivaciji. Optimizacijom karakteristika polimernih nosača ćelija, uslova kultivacije tipa i režima rada bioreaktora dobijeno je kultivisano tkivo hrskavice koje se po biohemijskim, strukturnim, morfološkim i biomehaničkim karakteristikama približava prirodnom tkivu.",
publisher = "Institut bezbednosti, Beograd",
journal = "Nauka, tehnika, bezbednost",
title = "In vitro tissue cultures: Microencapsulation and bioreactor cultivation, In vitro kulture tkiva - mikroenkapsulacija i bioreaktorska kultivacija",
pages = "68-59",
number = "2",
volume = "14",
url = "conv_1082"
}
Obradović, B., Bugarski, B., Bugarski, D.,& Vunjak-Novaković, G.. (2004). In vitro tissue cultures: Microencapsulation and bioreactor cultivation. in Nauka, tehnika, bezbednost
Institut bezbednosti, Beograd., 14(2), 59-68.
conv_1082
Obradović B, Bugarski B, Bugarski D, Vunjak-Novaković G. In vitro tissue cultures: Microencapsulation and bioreactor cultivation. in Nauka, tehnika, bezbednost. 2004;14(2):59-68.
conv_1082 .
Obradović, Bojana, Bugarski, Branko, Bugarski, Diana, Vunjak-Novaković, Gordana, "In vitro tissue cultures: Microencapsulation and bioreactor cultivation" in Nauka, tehnika, bezbednost, 14, no. 2 (2004):59-68,
conv_1082 .
