We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was depend...ent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
TY - JOUR
AU - Stančić, Ana
AU - Drvenica, Ivana
AU - Obradović, Hristina
AU - Bugarski, Branko
AU - Ilić, Vesna
AU - Bugarski, Diana
PY - 2020
UR - http://rimi.imi.bg.ac.rs/handle/123456789/1038
AB - We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
PB - Elsevier, Amsterdam
T2 - International Journal of Biological Macromolecules
T1 - Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro
EP - 920
SP - 909
VL - 144
DO - 10.1016/j.ijbiomac.2019.09.167
ER -
@article{
author = "Stančić, Ana and Drvenica, Ivana and Obradović, Hristina and Bugarski, Branko and Ilić, Vesna and Bugarski, Diana",
year = "2020",
abstract = "We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro",
pages = "920-909",
volume = "144",
doi = "10.1016/j.ijbiomac.2019.09.167"
}
Stančić, A., Drvenica, I., Obradović, H., Bugarski, B., Ilić, V.,& Bugarski, D.. (2020). Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 144, 909-920.
https://doi.org/10.1016/j.ijbiomac.2019.09.167
conv_4738
Stančić A, Drvenica I, Obradović H, Bugarski B, Ilić V, Bugarski D. Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules. 2020;144:909-920.
doi:10.1016/j.ijbiomac.2019.09.167
conv_4738 .
Stančić, Ana, Drvenica, Ivana, Obradović, Hristina, Bugarski, Branko, Ilić, Vesna, Bugarski, Diana, "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro" in International Journal of Biological Macromolecules, 144 (2020):909-920,
https://doi.org/10.1016/j.ijbiomac.2019.09.167 .,
conv_4738 .
