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Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes

Authorized Users Only
2020
Authors
Kovačić, Marijana
Fratrić, Natalija
Arsić, Aleksandra
Mojsilović, Slavko
Drvenica, Ivana
Marković, Dragana
Maslovarić, Irina
Grujić-Milanović, Jelica
Ivanov, Milan
Ilić, Vesna
Article (Published version)
Metadata
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Abstract
Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering disp...layed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.

Keywords:
Calves bronchopneumonia / Immune complexes / IgG / Lipids / Leukocytes
Source:
Research in Veterinary Science, 2020, 133, 63-74
Publisher:
  • Elsevier Sci Ltd, Oxford
Funding / projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200015 (University of Belgrade, Institute for Medical Research) (RS-200015)

DOI: 10.1016/j.rvsc.2020.09.004

ISSN: 0034-5288

PubMed: 32942254

WoS: 000600569200012

Scopus: 2-s2.0-85090714328
[ Google Scholar ]
URI
http://rimi.imi.bg.ac.rs/handle/123456789/1010
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Kovačić, Marijana
AU  - Fratrić, Natalija
AU  - Arsić, Aleksandra
AU  - Mojsilović, Slavko
AU  - Drvenica, Ivana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Grujić-Milanović, Jelica
AU  - Ivanov, Milan
AU  - Ilić, Vesna
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1010
AB  - Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes
EP  - 74
SP  - 63
VL  - 133
DO  - 10.1016/j.rvsc.2020.09.004
ER  - 
@article{
author = "Kovačić, Marijana and Fratrić, Natalija and Arsić, Aleksandra and Mojsilović, Slavko and Drvenica, Ivana and Marković, Dragana and Maslovarić, Irina and Grujić-Milanović, Jelica and Ivanov, Milan and Ilić, Vesna",
year = "2020",
abstract = "Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes",
pages = "74-63",
volume = "133",
doi = "10.1016/j.rvsc.2020.09.004"
}
Kovačić, M., Fratrić, N., Arsić, A., Mojsilović, S., Drvenica, I., Marković, D., Maslovarić, I., Grujić-Milanović, J., Ivanov, M.,& Ilić, V.. (2020). Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 133, 63-74.
https://doi.org/10.1016/j.rvsc.2020.09.004
conv_4935
Kovačić M, Fratrić N, Arsić A, Mojsilović S, Drvenica I, Marković D, Maslovarić I, Grujić-Milanović J, Ivanov M, Ilić V. Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science. 2020;133:63-74.
doi:10.1016/j.rvsc.2020.09.004
conv_4935 .
Kovačić, Marijana, Fratrić, Natalija, Arsić, Aleksandra, Mojsilović, Slavko, Drvenica, Ivana, Marković, Dragana, Maslovarić, Irina, Grujić-Milanović, Jelica, Ivanov, Milan, Ilić, Vesna, "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes" in Research in Veterinary Science, 133 (2020):63-74,
https://doi.org/10.1016/j.rvsc.2020.09.004 .,
conv_4935 .

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