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Platelet-poor plazma sportista kao potencijalni induktor miogene diferencijacije C2C12 mioblasta

dc.creatorMaslovarić, Irina
dc.creatorIlić, Vesna
dc.creatorStančić, Ana
dc.creatorSantibanez, Juan F.
dc.creatorTrivanović, Drenka
dc.creatorDrvenica, Ivana
dc.creatorKrstić, Jelena
dc.creatorMojsilović, Slavko
dc.creatorOkić Đorđević, Ivana
dc.creatorBugarski, Diana
dc.date.accessioned2021-04-20T13:06:47Z
dc.date.available2021-04-20T13:06:47Z
dc.date.issued2020
dc.identifier.issn0350-2457
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/1003
dc.description.abstractIntroduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months' frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters gt dancers gt football players; mean migratory capacity was: weightlifters = dancers gt football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players gt dancers gt weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, longterm, frozen-stored PPP preserves its potential to modify myoblast morphology and function.en
dc.description.abstractUvod. Krvna plazma obogaćena leukocitima, plazma sa niskim sadržajem leukocita i plazma sa niskim sadržajem trombocita (platelet poor plasma; PPP) su produkti krvi koji se koriste za stimulaciju regeneracije mišića. U ovom radu smo ispitivali da li zamrzavana PPP osoba koje se bave različitim tipovima fizičke aktivnosti, usmerava C2C12 myoblaste u pravcu povećane proliferacije, migracije i miogene diferencijacije, i da li utiče na morfologiju/izgled miotuba. Materijal i metode. PPP osoba muškog pola starih 15-19 godina je izolovana iz krvi dizača tegova, fudbalera i profesionalnih igrača folklora, 12 sati nakon treninga. Uzorci PPP su čuvani šest meseci na -20ºC. Uticaj PPP na proliferaciju C2C12 ćelija je analiziran MTT testom, na migraciju "scratch" testom, a uticaj na miogenu diferencijaciju je analiziran na osnovu sposobnosti PPP da indukuju formiranje miotuba. Želatinolitička aktivnost PPP je analizirana gel-zimografijom. Rezultati i zaključak. Uzorci PPP su indukovali proliferaciju i migraciju C2C12 ćelija, a kapacitet da stimulišu proliferaciju je bio: dizači tegova gt igrači gt fudbaleri. Kapacitet PPP da utiču na migraciju C2C12 ćelija je bio: dizači tegova = igrači gt fudbaleri. Svi uzorci PPP su indukovali formiranje miotuba, ali su zapažene značajne interindividualne varijacije. PPP dizača tegova su indukovali formiranje okruglih miotuba, dok su miotube formirane u prisustvu PPP igrača i fudbalera bile izdužene. Površina miotuba se, zavisno od tipa fizičke aktivnosti, menjala po sledećem rasporedu: fudbaleri gt igrači gt dizači tegova. Želatinolitička aktivnost PPP je nagativno korelirala sa proliferacijom C2C12 ćelija. Rezultati ove studije pokazuju da PPP osoba koje se bave određenim tipom fizičke aktivnosti mogu da na specifičan način modulišu morfologiju/funciju mioblasta. Ovaj rezultat je od značaja za objašnjnje fiziološkog odgovora i adaptacije na vežbanje. On pokazuje i da PPP nakon dugotrajnog zamrzavanja imaju očuvanu spospbnost modifikovanja morfologije i funkcije mioblasta.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.relationThis work was supported by grants from the Ministry of Youth and Sport, Republic of Serbia (Contract No. 450-451/02-012713/1/2011-03)
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceVeterinarski glasnik
dc.subjectC2C12 myoblastsen
dc.subjectExerciseen
dc.subjectMyogenic differentiationen
dc.subjectMyotube morphologyen
dc.subjectC2C12 mioblastisr
dc.subjectfizičko vežbanjesr
dc.subjectmiogena diferencijacijasr
dc.subjectmorfologija miotubasr
dc.titlePlatelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblastsen
dc.titlePlatelet-poor plazma sportista kao potencijalni induktor miogene diferencijacije C2C12 mioblastasr
dc.typearticle
dc.rights.licenseBY
dc.citation.epage33
dc.citation.issue1
dc.citation.other74(1): 18-33
dc.citation.rankM51~
dc.citation.spage18
dc.citation.volume74
dc.identifier.doi10.2298/VETGL190414019M
dc.identifier.fulltexthttp://rimi.imi.bg.ac.rs/bitstream/id/1/1000.pdf
dc.type.versionpublishedVersion


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