Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia
Само за регистроване кориснике
2017
Аутори
Kuruca, LjiljanaKlun, Ivana
Uzelac, Aleksandra
Nikolić, Aleksandra
Bobić, Branko
Simin, Stanislav
Lalošević, Vesna
Lalošević, Dušan
Đurković-Đaković, Olgica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Insufficiently cooked pork is considered as an important source of human infection with Toxoplasma gondii. The aim of our study was to investigate the presence of T. gondii in pigs intended for human consumption from Northern Serbia. Blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. Sera were examined using modified agglutination test (MAT), and diaphragms from seropositive, as well as from some MAT-negative pigs, were bioassayed in mice. In addition, digests were examined for the presence of T. gondii DNA using a real-time polymerase chain reaction (qPCR) which was targeted at the 529 bp repetitive element of the T. gondii genome. The overall seroprevalence of T. gondii in pigs was 17% (31/182), with no difference between pigs from large commercial farms (17.8%) and those raised on smallholdings (16.3%). However, the seroprevalence in farm pigs was largely influenced by the findings on a... single farm, where all examined animals tested positive. Parasites and/or parasite DNA were detected in the tissues of 15 of the 45 (25 seropositive and 20 seronegative) animals examined by either direct method. Tissue cysts were isolated in eight bioassays and an additional bioassay was positive by serology; all nine were confirmed positive by qPCR. All positive bioassays originated from seropositive pigs, but no correlation was observed between isolation rate and antibody titer. T. gondii DNA was detected in diaphragm tissues of eight pigs, of which three were seronegative. The results of our study provide further evidence for pork as a source of human T. gondii infection.
Кључне речи:
Toxoplasma gondii / Pigs / Farms / Smallholdings / Isolation / SerbiaИзвор:
Parasitology Research, 2017, 116, 11, 3117-3123Издавач:
- Springer, New York
Финансирање / пројекти:
- Одабране биолошке опасности за безбедност/квалитет хране анималног порекла и контролне мере од фарме до потрошача (RS-MESTD-Technological Development (TD or TR)-31034)
- Контрола инфекција апикомплексним патогенима: од нових места деловања лека до предикције (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41019)
DOI: 10.1007/s00436-017-5623-7
ISSN: 0932-0113
PubMed: 28956155
WoS: 000413979600026
Scopus: 2-s2.0-85030129658
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Kuruca, Ljiljana AU - Klun, Ivana AU - Uzelac, Aleksandra AU - Nikolić, Aleksandra AU - Bobić, Branko AU - Simin, Stanislav AU - Lalošević, Vesna AU - Lalošević, Dušan AU - Đurković-Đaković, Olgica PY - 2017 UR - http://rimi.imi.bg.ac.rs/handle/123456789/793 AB - Insufficiently cooked pork is considered as an important source of human infection with Toxoplasma gondii. The aim of our study was to investigate the presence of T. gondii in pigs intended for human consumption from Northern Serbia. Blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. Sera were examined using modified agglutination test (MAT), and diaphragms from seropositive, as well as from some MAT-negative pigs, were bioassayed in mice. In addition, digests were examined for the presence of T. gondii DNA using a real-time polymerase chain reaction (qPCR) which was targeted at the 529 bp repetitive element of the T. gondii genome. The overall seroprevalence of T. gondii in pigs was 17% (31/182), with no difference between pigs from large commercial farms (17.8%) and those raised on smallholdings (16.3%). However, the seroprevalence in farm pigs was largely influenced by the findings on a single farm, where all examined animals tested positive. Parasites and/or parasite DNA were detected in the tissues of 15 of the 45 (25 seropositive and 20 seronegative) animals examined by either direct method. Tissue cysts were isolated in eight bioassays and an additional bioassay was positive by serology; all nine were confirmed positive by qPCR. All positive bioassays originated from seropositive pigs, but no correlation was observed between isolation rate and antibody titer. T. gondii DNA was detected in diaphragm tissues of eight pigs, of which three were seronegative. The results of our study provide further evidence for pork as a source of human T. gondii infection. PB - Springer, New York T2 - Parasitology Research T1 - Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia EP - 3123 IS - 11 SP - 3117 VL - 116 DO - 10.1007/s00436-017-5623-7 ER -
@article{ author = "Kuruca, Ljiljana and Klun, Ivana and Uzelac, Aleksandra and Nikolić, Aleksandra and Bobić, Branko and Simin, Stanislav and Lalošević, Vesna and Lalošević, Dušan and Đurković-Đaković, Olgica", year = "2017", abstract = "Insufficiently cooked pork is considered as an important source of human infection with Toxoplasma gondii. The aim of our study was to investigate the presence of T. gondii in pigs intended for human consumption from Northern Serbia. Blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. Sera were examined using modified agglutination test (MAT), and diaphragms from seropositive, as well as from some MAT-negative pigs, were bioassayed in mice. In addition, digests were examined for the presence of T. gondii DNA using a real-time polymerase chain reaction (qPCR) which was targeted at the 529 bp repetitive element of the T. gondii genome. The overall seroprevalence of T. gondii in pigs was 17% (31/182), with no difference between pigs from large commercial farms (17.8%) and those raised on smallholdings (16.3%). However, the seroprevalence in farm pigs was largely influenced by the findings on a single farm, where all examined animals tested positive. Parasites and/or parasite DNA were detected in the tissues of 15 of the 45 (25 seropositive and 20 seronegative) animals examined by either direct method. Tissue cysts were isolated in eight bioassays and an additional bioassay was positive by serology; all nine were confirmed positive by qPCR. All positive bioassays originated from seropositive pigs, but no correlation was observed between isolation rate and antibody titer. T. gondii DNA was detected in diaphragm tissues of eight pigs, of which three were seronegative. The results of our study provide further evidence for pork as a source of human T. gondii infection.", publisher = "Springer, New York", journal = "Parasitology Research", title = "Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia", pages = "3123-3117", number = "11", volume = "116", doi = "10.1007/s00436-017-5623-7" }
Kuruca, L., Klun, I., Uzelac, A., Nikolić, A., Bobić, B., Simin, S., Lalošević, V., Lalošević, D.,& Đurković-Đaković, O.. (2017). Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia. in Parasitology Research Springer, New York., 116(11), 3117-3123. https://doi.org/10.1007/s00436-017-5623-7
Kuruca L, Klun I, Uzelac A, Nikolić A, Bobić B, Simin S, Lalošević V, Lalošević D, Đurković-Đaković O. Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia. in Parasitology Research. 2017;116(11):3117-3123. doi:10.1007/s00436-017-5623-7 .
Kuruca, Ljiljana, Klun, Ivana, Uzelac, Aleksandra, Nikolić, Aleksandra, Bobić, Branko, Simin, Stanislav, Lalošević, Vesna, Lalošević, Dušan, Đurković-Đaković, Olgica, "Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia" in Parasitology Research, 116, no. 11 (2017):3117-3123, https://doi.org/10.1007/s00436-017-5623-7 . .