Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations
Samo za registrovane korisnike
2016
Autori
Krga, IrenaMonfoulet, Laurent-Emanuel
Konić-Ristić, Aleksandra
Mercier, Sylvie
Glibetić, Marija
Morand, Christine
Milenković, Dragan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
An increasing number of evidence suggests a protective role of dietary anthocyanins against cardiovascular diseases. Anthocyanins' extensive metabolism indicates that their metabolites could be responsible for the protective effects associated with consumption of anthocyanin-rich foods. The aim of this work was to investigate the effect of plasma anthocyanins and their metabolites on the adhesion of monocytes to TNF alpha-activated endothelial cells and on the expression of genes encoding cell adhesion molecules. Human umbilical vein endothelial cells (HUVECs) were exposed to circulating anthocyanins: cyanidin-3-arabinoside, cyanidin-3-galactoside, cyanidin-3-glucoside, delphinidin-3-glucoside, peonidin-3-glucoside, anthocyanin degradation product: 4-hydroxybenzaidehyde, or to their gut metabolites: protocatechuic, vanillic, ferulic and hippuric acid, at physiologically-relevant concentrations (0.1-2 mu M) and time of exposure. Both anthocyanins and gut metabolites decreased the adhesi...on of monocytes to HUVECs, with a magnitude ranging from 18.1% to 47%. The mixture of anthocyanins and that of gut metabolites also reduced monocyte adhesion. However, no significant effect on the expression of genes encoding E-selectin, ICAM1 and VCAM1 was observed, suggesting that other molecular targets are involved in the observed effect. In conclusion, this study showed the potency of anthocyanins and their gut metabolites to modulate the adhesion of monocytes to endothelial cells, the initial step in atherosclerosis development, under physiologically-relevant conditions.
Ključne reči:
Anthocyanins / Gut metabolites / Monocyte adhesion / Endothelial cells / Gene expression / Cell adhesion moleculesIzvor:
Archives of Biochemistry & Biophysics, 2016, 599, 51-59Izdavač:
- Elsevier Science Inc, New York
DOI: 10.1016/j.abb.2016.02.006
ISSN: 0003-9861
PubMed: 26873533
WoS: 000376810900007
Scopus: 2-s2.0-84957698592
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Krga, Irena AU - Monfoulet, Laurent-Emanuel AU - Konić-Ristić, Aleksandra AU - Mercier, Sylvie AU - Glibetić, Marija AU - Morand, Christine AU - Milenković, Dragan PY - 2016 UR - http://rimi.imi.bg.ac.rs/handle/123456789/718 AB - An increasing number of evidence suggests a protective role of dietary anthocyanins against cardiovascular diseases. Anthocyanins' extensive metabolism indicates that their metabolites could be responsible for the protective effects associated with consumption of anthocyanin-rich foods. The aim of this work was to investigate the effect of plasma anthocyanins and their metabolites on the adhesion of monocytes to TNF alpha-activated endothelial cells and on the expression of genes encoding cell adhesion molecules. Human umbilical vein endothelial cells (HUVECs) were exposed to circulating anthocyanins: cyanidin-3-arabinoside, cyanidin-3-galactoside, cyanidin-3-glucoside, delphinidin-3-glucoside, peonidin-3-glucoside, anthocyanin degradation product: 4-hydroxybenzaidehyde, or to their gut metabolites: protocatechuic, vanillic, ferulic and hippuric acid, at physiologically-relevant concentrations (0.1-2 mu M) and time of exposure. Both anthocyanins and gut metabolites decreased the adhesion of monocytes to HUVECs, with a magnitude ranging from 18.1% to 47%. The mixture of anthocyanins and that of gut metabolites also reduced monocyte adhesion. However, no significant effect on the expression of genes encoding E-selectin, ICAM1 and VCAM1 was observed, suggesting that other molecular targets are involved in the observed effect. In conclusion, this study showed the potency of anthocyanins and their gut metabolites to modulate the adhesion of monocytes to endothelial cells, the initial step in atherosclerosis development, under physiologically-relevant conditions. PB - Elsevier Science Inc, New York T2 - Archives of Biochemistry & Biophysics T1 - Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations EP - 59 SP - 51 VL - 599 DO - 10.1016/j.abb.2016.02.006 ER -
@article{ author = "Krga, Irena and Monfoulet, Laurent-Emanuel and Konić-Ristić, Aleksandra and Mercier, Sylvie and Glibetić, Marija and Morand, Christine and Milenković, Dragan", year = "2016", abstract = "An increasing number of evidence suggests a protective role of dietary anthocyanins against cardiovascular diseases. Anthocyanins' extensive metabolism indicates that their metabolites could be responsible for the protective effects associated with consumption of anthocyanin-rich foods. The aim of this work was to investigate the effect of plasma anthocyanins and their metabolites on the adhesion of monocytes to TNF alpha-activated endothelial cells and on the expression of genes encoding cell adhesion molecules. Human umbilical vein endothelial cells (HUVECs) were exposed to circulating anthocyanins: cyanidin-3-arabinoside, cyanidin-3-galactoside, cyanidin-3-glucoside, delphinidin-3-glucoside, peonidin-3-glucoside, anthocyanin degradation product: 4-hydroxybenzaidehyde, or to their gut metabolites: protocatechuic, vanillic, ferulic and hippuric acid, at physiologically-relevant concentrations (0.1-2 mu M) and time of exposure. Both anthocyanins and gut metabolites decreased the adhesion of monocytes to HUVECs, with a magnitude ranging from 18.1% to 47%. The mixture of anthocyanins and that of gut metabolites also reduced monocyte adhesion. However, no significant effect on the expression of genes encoding E-selectin, ICAM1 and VCAM1 was observed, suggesting that other molecular targets are involved in the observed effect. In conclusion, this study showed the potency of anthocyanins and their gut metabolites to modulate the adhesion of monocytes to endothelial cells, the initial step in atherosclerosis development, under physiologically-relevant conditions.", publisher = "Elsevier Science Inc, New York", journal = "Archives of Biochemistry & Biophysics", title = "Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations", pages = "59-51", volume = "599", doi = "10.1016/j.abb.2016.02.006" }
Krga, I., Monfoulet, L., Konić-Ristić, A., Mercier, S., Glibetić, M., Morand, C.,& Milenković, D.. (2016). Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations. in Archives of Biochemistry & Biophysics Elsevier Science Inc, New York., 599, 51-59. https://doi.org/10.1016/j.abb.2016.02.006
Krga I, Monfoulet L, Konić-Ristić A, Mercier S, Glibetić M, Morand C, Milenković D. Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations. in Archives of Biochemistry & Biophysics. 2016;599:51-59. doi:10.1016/j.abb.2016.02.006 .
Krga, Irena, Monfoulet, Laurent-Emanuel, Konić-Ristić, Aleksandra, Mercier, Sylvie, Glibetić, Marija, Morand, Christine, Milenković, Dragan, "Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations" in Archives of Biochemistry & Biophysics, 599 (2016):51-59, https://doi.org/10.1016/j.abb.2016.02.006 . .