Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation
Само за регистроване кориснике
2016
Аутори
Kovačić, MarijanaMojsilović, Slavko
Mišić, Dušan
Vuković, Dejan
Savić, Olivera
Valčić, Olivera
Marković, Dragana
Gvozdić, Dragan
Ilić, Vesna
Fratrić, Natalija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows' granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes' viability. The strongest effect of inhibition of cows' granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves' MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, thei...r inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves' MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
Кључне речи:
Calf bronchopneumonia / Circulating immune complexes / Peripheral blood leukocytesИзвор:
Research in Veterinary Science, 2016, 106, 135-142Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-46002)
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-175062)
DOI: 10.1016/j.rvsc.2016.04.002
ISSN: 0034-5288
PubMed: 27234551
WoS: 000377923400024
Scopus: 2-s2.0-84963699389
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Kovačić, Marijana AU - Mojsilović, Slavko AU - Mišić, Dušan AU - Vuković, Dejan AU - Savić, Olivera AU - Valčić, Olivera AU - Marković, Dragana AU - Gvozdić, Dragan AU - Ilić, Vesna AU - Fratrić, Natalija PY - 2016 UR - http://rimi.imi.bg.ac.rs/handle/123456789/717 AB - In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows' granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes' viability. The strongest effect of inhibition of cows' granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves' MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves' MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence. PB - Elsevier Sci Ltd, Oxford T2 - Research in Veterinary Science T1 - Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation EP - 142 SP - 135 VL - 106 DO - 10.1016/j.rvsc.2016.04.002 ER -
@article{ author = "Kovačić, Marijana and Mojsilović, Slavko and Mišić, Dušan and Vuković, Dejan and Savić, Olivera and Valčić, Olivera and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna and Fratrić, Natalija", year = "2016", abstract = "In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows' granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes' viability. The strongest effect of inhibition of cows' granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves' MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves' MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Research in Veterinary Science", title = "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation", pages = "142-135", volume = "106", doi = "10.1016/j.rvsc.2016.04.002" }
Kovačić, M., Mojsilović, S., Mišić, D., Vuković, D., Savić, O., Valčić, O., Marković, D., Gvozdić, D., Ilić, V.,& Fratrić, N.. (2016). Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science Elsevier Sci Ltd, Oxford., 106, 135-142. https://doi.org/10.1016/j.rvsc.2016.04.002
Kovačić M, Mojsilović S, Mišić D, Vuković D, Savić O, Valčić O, Marković D, Gvozdić D, Ilić V, Fratrić N. Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science. 2016;106:135-142. doi:10.1016/j.rvsc.2016.04.002 .
Kovačić, Marijana, Mojsilović, Slavko, Mišić, Dušan, Vuković, Dejan, Savić, Olivera, Valčić, Olivera, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, Fratrić, Natalija, "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation" in Research in Veterinary Science, 106 (2016):135-142, https://doi.org/10.1016/j.rvsc.2016.04.002 . .