SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA
2015
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Prikaz svih podataka o dokumentuApstrakt
In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofluorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was significantly more sensitive than IFA (84.8 vs 67.9%), whereas the specificities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically... insignificant. These results are the first report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population.
Ključne reči:
Borrelia burgdorferi / dogs / IFA / ELISA / Western blot / seroprevalenceIzvor:
Acta Veterinaria-Beograd, 2015, 65, 1, 99-110Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Finansiranje / projekti:
- Regenerativni i modulatorni potencijal adultnih matičnih ćelija (RS-MESTD-Basic Research (BR or ON)-175062)
- Enzootski transmisioni ciklusi patogenih mikroorganizama koje prenose krpelji (RS-MESTD-Basic Research (BR or ON)-173006)
- Razvoj i standardizacija molekularnih i imunskih metoda u dijagnostici bakterijskih zoonoza (RS-MESTD-Technological Development (TD or TR)-31088)
DOI: 10.1515/acve-2015-0008
ISSN: 0567-8315
WoS: 000351737400008
Scopus: 2-s2.0-84927625550
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Obrenović, Sonja AU - Ristanović, Elizabeta AU - Cekanac, Radovan AU - Radulović, Željko AU - Ilić, Vesna PY - 2015 UR - http://rimi.imi.bg.ac.rs/handle/123456789/657 AB - In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofluorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was significantly more sensitive than IFA (84.8 vs 67.9%), whereas the specificities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically insignificant. These results are the first report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA EP - 110 IS - 1 SP - 99 VL - 65 DO - 10.1515/acve-2015-0008 ER -
@article{ author = "Obrenović, Sonja and Ristanović, Elizabeta and Cekanac, Radovan and Radulović, Željko and Ilić, Vesna", year = "2015", abstract = "In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofluorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was significantly more sensitive than IFA (84.8 vs 67.9%), whereas the specificities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically insignificant. These results are the first report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA", pages = "110-99", number = "1", volume = "65", doi = "10.1515/acve-2015-0008" }
Obrenović, S., Ristanović, E., Cekanac, R., Radulović, Ž.,& Ilić, V.. (2015). SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1), 99-110. https://doi.org/10.1515/acve-2015-0008
Obrenović S, Ristanović E, Cekanac R, Radulović Ž, Ilić V. SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA. in Acta Veterinaria-Beograd. 2015;65(1):99-110. doi:10.1515/acve-2015-0008 .
Obrenović, Sonja, Ristanović, Elizabeta, Cekanac, Radovan, Radulović, Željko, Ilić, Vesna, "SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA" in Acta Veterinaria-Beograd, 65, no. 1 (2015):99-110, https://doi.org/10.1515/acve-2015-0008 . .