Mesenchymal stem cells isolated from human periodontal ligament
2014
Autori
Miletić, MajaMojsilović, S.
Okić Đorđević, Ivana
Kukolj, Tamara
Jauković, Aleksandra
Santibanez, Juan F.
Jovčić, Gordana
Bugarski, Diana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells ...should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.
Ključne reči:
Periodontal ligament / mesenchymal stem cells / characterization / proliferation / differentiation / tissue regenerationIzvor:
Archives of Biological Sciences, 2014, 66, 1, 261-271Izdavač:
- Srpsko biološko društvo, Beograd, i dr.
Finansiranje / projekti:
- Regenerativni i modulatorni potencijal adultnih matičnih ćelija (RS-175062)
- Primene niskotemperaturnih plazmi u biomedicini, zaštiti čovekove okoline i nanotehnologijama (RS-41011)
DOI: 10.2298/ABS1401261M
ISSN: 0354-4664
WoS: 000330552400035
Scopus: 2-s2.0-84892417888
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Miletić, Maja AU - Mojsilović, S. AU - Okić Đorđević, Ivana AU - Kukolj, Tamara AU - Jauković, Aleksandra AU - Santibanez, Juan F. AU - Jovčić, Gordana AU - Bugarski, Diana PY - 2014 UR - http://rimi.imi.bg.ac.rs/handle/123456789/599 AB - Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Mesenchymal stem cells isolated from human periodontal ligament EP - 271 IS - 1 SP - 261 VL - 66 DO - 10.2298/ABS1401261M ER -
@article{ author = "Miletić, Maja and Mojsilović, S. and Okić Đorđević, Ivana and Kukolj, Tamara and Jauković, Aleksandra and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana", year = "2014", abstract = "Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Mesenchymal stem cells isolated from human periodontal ligament", pages = "271-261", number = "1", volume = "66", doi = "10.2298/ABS1401261M" }
Miletić, M., Mojsilović, S., Okić Đorđević, I., Kukolj, T., Jauković, A., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2014). Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 66(1), 261-271. https://doi.org/10.2298/ABS1401261M
Miletić M, Mojsilović S, Okić Đorđević I, Kukolj T, Jauković A, Santibanez JF, Jovčić G, Bugarski D. Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences. 2014;66(1):261-271. doi:10.2298/ABS1401261M .
Miletić, Maja, Mojsilović, S., Okić Đorđević, Ivana, Kukolj, Tamara, Jauković, Aleksandra, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Mesenchymal stem cells isolated from human periodontal ligament" in Archives of Biological Sciences, 66, no. 1 (2014):261-271, https://doi.org/10.2298/ABS1401261M . .