Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae
2014
Аутори
Ćirković, IvanaPavlović, Ljiljana
Konstantinović, Neda M.
Kostić, Katarina
Jovanović, Snežana
Đukić, Slobodanka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction Beta-lactam antibiotics are the most commonly used antibacterial drugs. However, many bacteria have developed resistance to these antibiotics, and the most common form of resistance. is the production of beta-lactamase enzymes. Many members of the Enterobacteriaceae family produce different types of these enzymes. Objective The aim of this study was to perform phenotypic detection of production and identification of beta-lactamase type in Enterobacteriaceae isolated from different clinical specimens from patients hospitalized in the Clinical Center of Serbia. Methods The strains of Enterobacteriaceae were collected between November 2011 and January 2012 in the laboratory of the Clinical Center of Serbia. The isolates were identified according to the standard microbiology procedures and confirmed by the Vitek2 automated system. Antimicrobial susceptibility testing was performed by the disk diffusion method, and the phenotypic detection of production and identification of be...ta-lactamases was performed according to previously described methodologies. Results In this study, a total of 172 Enterobacteriaceae strains were isolated. Further testing was performed on 54/145 (37.2%) strains showing decreased susceptibility to beta-lactam antibiotics: 13/85 (15.3%) Escherichia coli, 31/46 (67.4%) Klebsiella pneumoniae and 10/14(71.4%) Proteus mirabilis. Among them, 40/145 (27.6%) strains produced extended spectrum beta-lactamases (ESBLs), 9/145 (6.2%) - AmpC, 1/145 (0.7%) - K1 beta-lactamase and 4/145 (2.8%) - carbapenemases. Carbapenemases were predominantly detected in K. pneumoniae (75%). Conclusion Enterobacteriaceae produce different types of beta-lactamases, and the most common type in our study was ESBLs. Production of carbapenemases detected in Enterobacteriaceae is also an associated problem.
Кључне речи:
beta-lactamases / enterobacteria / resistanceИзвор:
Srpski arhiv za celokupno lekarstvo, 2014, 142, 7-8, 457-463Издавач:
- Srpsko lekarsko društvo, Beograd
Финансирање / пројекти:
- Бактерије резистентне на антибиотике у Србији: фенотипска и генотипска карактеризација (RS-MESTD-Basic Research (BR or ON)-175039)
DOI: 10.2298/SARH1408457C
ISSN: 0370-8179
PubMed: 25233691
WoS: 000341069800009
Scopus: 2-s2.0-84922622956
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Ćirković, Ivana AU - Pavlović, Ljiljana AU - Konstantinović, Neda M. AU - Kostić, Katarina AU - Jovanović, Snežana AU - Đukić, Slobodanka PY - 2014 UR - http://rimi.imi.bg.ac.rs/handle/123456789/546 AB - Introduction Beta-lactam antibiotics are the most commonly used antibacterial drugs. However, many bacteria have developed resistance to these antibiotics, and the most common form of resistance. is the production of beta-lactamase enzymes. Many members of the Enterobacteriaceae family produce different types of these enzymes. Objective The aim of this study was to perform phenotypic detection of production and identification of beta-lactamase type in Enterobacteriaceae isolated from different clinical specimens from patients hospitalized in the Clinical Center of Serbia. Methods The strains of Enterobacteriaceae were collected between November 2011 and January 2012 in the laboratory of the Clinical Center of Serbia. The isolates were identified according to the standard microbiology procedures and confirmed by the Vitek2 automated system. Antimicrobial susceptibility testing was performed by the disk diffusion method, and the phenotypic detection of production and identification of beta-lactamases was performed according to previously described methodologies. Results In this study, a total of 172 Enterobacteriaceae strains were isolated. Further testing was performed on 54/145 (37.2%) strains showing decreased susceptibility to beta-lactam antibiotics: 13/85 (15.3%) Escherichia coli, 31/46 (67.4%) Klebsiella pneumoniae and 10/14(71.4%) Proteus mirabilis. Among them, 40/145 (27.6%) strains produced extended spectrum beta-lactamases (ESBLs), 9/145 (6.2%) - AmpC, 1/145 (0.7%) - K1 beta-lactamase and 4/145 (2.8%) - carbapenemases. Carbapenemases were predominantly detected in K. pneumoniae (75%). Conclusion Enterobacteriaceae produce different types of beta-lactamases, and the most common type in our study was ESBLs. Production of carbapenemases detected in Enterobacteriaceae is also an associated problem. PB - Srpsko lekarsko društvo, Beograd T2 - Srpski arhiv za celokupno lekarstvo T1 - Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae EP - 463 IS - 7-8 SP - 457 VL - 142 DO - 10.2298/SARH1408457C ER -
@article{ author = "Ćirković, Ivana and Pavlović, Ljiljana and Konstantinović, Neda M. and Kostić, Katarina and Jovanović, Snežana and Đukić, Slobodanka", year = "2014", abstract = "Introduction Beta-lactam antibiotics are the most commonly used antibacterial drugs. However, many bacteria have developed resistance to these antibiotics, and the most common form of resistance. is the production of beta-lactamase enzymes. Many members of the Enterobacteriaceae family produce different types of these enzymes. Objective The aim of this study was to perform phenotypic detection of production and identification of beta-lactamase type in Enterobacteriaceae isolated from different clinical specimens from patients hospitalized in the Clinical Center of Serbia. Methods The strains of Enterobacteriaceae were collected between November 2011 and January 2012 in the laboratory of the Clinical Center of Serbia. The isolates were identified according to the standard microbiology procedures and confirmed by the Vitek2 automated system. Antimicrobial susceptibility testing was performed by the disk diffusion method, and the phenotypic detection of production and identification of beta-lactamases was performed according to previously described methodologies. Results In this study, a total of 172 Enterobacteriaceae strains were isolated. Further testing was performed on 54/145 (37.2%) strains showing decreased susceptibility to beta-lactam antibiotics: 13/85 (15.3%) Escherichia coli, 31/46 (67.4%) Klebsiella pneumoniae and 10/14(71.4%) Proteus mirabilis. Among them, 40/145 (27.6%) strains produced extended spectrum beta-lactamases (ESBLs), 9/145 (6.2%) - AmpC, 1/145 (0.7%) - K1 beta-lactamase and 4/145 (2.8%) - carbapenemases. Carbapenemases were predominantly detected in K. pneumoniae (75%). Conclusion Enterobacteriaceae produce different types of beta-lactamases, and the most common type in our study was ESBLs. Production of carbapenemases detected in Enterobacteriaceae is also an associated problem.", publisher = "Srpsko lekarsko društvo, Beograd", journal = "Srpski arhiv za celokupno lekarstvo", title = "Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae", pages = "463-457", number = "7-8", volume = "142", doi = "10.2298/SARH1408457C" }
Ćirković, I., Pavlović, L., Konstantinović, N. M., Kostić, K., Jovanović, S.,& Đukić, S.. (2014). Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae. in Srpski arhiv za celokupno lekarstvo Srpsko lekarsko društvo, Beograd., 142(7-8), 457-463. https://doi.org/10.2298/SARH1408457C
Ćirković I, Pavlović L, Konstantinović NM, Kostić K, Jovanović S, Đukić S. Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae. in Srpski arhiv za celokupno lekarstvo. 2014;142(7-8):457-463. doi:10.2298/SARH1408457C .
Ćirković, Ivana, Pavlović, Ljiljana, Konstantinović, Neda M., Kostić, Katarina, Jovanović, Snežana, Đukić, Slobodanka, "Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae" in Srpski arhiv za celokupno lekarstvo, 142, no. 7-8 (2014):457-463, https://doi.org/10.2298/SARH1408457C . .