Evidence for genetic diversity of Toxoplasma gondii in selected intermediate hosts in Serbia
Само за регистроване кориснике
2014
Аутори
Marković, MarijaIvović, Vladimir
Štajner, Tijana
Đokić, Vitomir
Klun, Ivana
Bobić, Branko
Nikolić, Aleksandra
Đurković-Đaković, Olgica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
To contribute to the insight into the worldwide population structure of Toxoplasma gondii, we genetically characterized a total of eight strains isolated from intermediate hosts including humans, sheep and pigeons in Serbia. Although parasite DNA was detected in 28.2% (60/213) of the human samples from 162 patients serologically suspected of active toxoplasmosis, as well as in 5/7 seropositive pigeons and in 2/12 seropositive sheep examined, multilocus PCR-RFLP genotyping, using SAGI, 5'SAG2, 3'SAG2, GRA6, 5'GRA7 and 3'GRA7 as markers, was successful in only four human isolates (of which one was isolated from both the bronchoalveolar lavage fluid and blood samples of a single patient), one sheep and three pigeons. Of the eight isolates, five were type II (62.5%), one was type III, one was atypical, and one had a type I allele at GRA6 as the single locus genotyped. Although type II, as elsewhere in Europe, predominated, these results may suggest a higher genetic diversity of T. gondii i...n Serbia, reflecting local environmental contamination and also the geographical position of the country in South-East Europe.
Кључне речи:
Toxoplasma gondii / Intermediate hosts / Humans / Sheep / Pigeons / Genotyping / PCR-RFLP / Serbia / South-East EuropeИзвор:
Comparative Immunology Microbiology & Infectious Diseases, 2014, 37, 3, 173-179Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Контрола инфекција апикомплексним патогенима: од нових места деловања лека до предикције (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41019)
DOI: 10.1016/j.cimid.2014.03.001
ISSN: 0147-9571
PubMed: 24698636
WoS: 000338603600005
Scopus: 2-s2.0-84901977474
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Marković, Marija AU - Ivović, Vladimir AU - Štajner, Tijana AU - Đokić, Vitomir AU - Klun, Ivana AU - Bobić, Branko AU - Nikolić, Aleksandra AU - Đurković-Đaković, Olgica PY - 2014 UR - http://rimi.imi.bg.ac.rs/handle/123456789/539 AB - To contribute to the insight into the worldwide population structure of Toxoplasma gondii, we genetically characterized a total of eight strains isolated from intermediate hosts including humans, sheep and pigeons in Serbia. Although parasite DNA was detected in 28.2% (60/213) of the human samples from 162 patients serologically suspected of active toxoplasmosis, as well as in 5/7 seropositive pigeons and in 2/12 seropositive sheep examined, multilocus PCR-RFLP genotyping, using SAGI, 5'SAG2, 3'SAG2, GRA6, 5'GRA7 and 3'GRA7 as markers, was successful in only four human isolates (of which one was isolated from both the bronchoalveolar lavage fluid and blood samples of a single patient), one sheep and three pigeons. Of the eight isolates, five were type II (62.5%), one was type III, one was atypical, and one had a type I allele at GRA6 as the single locus genotyped. Although type II, as elsewhere in Europe, predominated, these results may suggest a higher genetic diversity of T. gondii in Serbia, reflecting local environmental contamination and also the geographical position of the country in South-East Europe. PB - Elsevier Sci Ltd, Oxford T2 - Comparative Immunology Microbiology & Infectious Diseases T1 - Evidence for genetic diversity of Toxoplasma gondii in selected intermediate hosts in Serbia EP - 179 IS - 3 SP - 173 VL - 37 DO - 10.1016/j.cimid.2014.03.001 ER -
@article{ author = "Marković, Marija and Ivović, Vladimir and Štajner, Tijana and Đokić, Vitomir and Klun, Ivana and Bobić, Branko and Nikolić, Aleksandra and Đurković-Đaković, Olgica", year = "2014", abstract = "To contribute to the insight into the worldwide population structure of Toxoplasma gondii, we genetically characterized a total of eight strains isolated from intermediate hosts including humans, sheep and pigeons in Serbia. Although parasite DNA was detected in 28.2% (60/213) of the human samples from 162 patients serologically suspected of active toxoplasmosis, as well as in 5/7 seropositive pigeons and in 2/12 seropositive sheep examined, multilocus PCR-RFLP genotyping, using SAGI, 5'SAG2, 3'SAG2, GRA6, 5'GRA7 and 3'GRA7 as markers, was successful in only four human isolates (of which one was isolated from both the bronchoalveolar lavage fluid and blood samples of a single patient), one sheep and three pigeons. Of the eight isolates, five were type II (62.5%), one was type III, one was atypical, and one had a type I allele at GRA6 as the single locus genotyped. Although type II, as elsewhere in Europe, predominated, these results may suggest a higher genetic diversity of T. gondii in Serbia, reflecting local environmental contamination and also the geographical position of the country in South-East Europe.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Comparative Immunology Microbiology & Infectious Diseases", title = "Evidence for genetic diversity of Toxoplasma gondii in selected intermediate hosts in Serbia", pages = "179-173", number = "3", volume = "37", doi = "10.1016/j.cimid.2014.03.001" }
Marković, M., Ivović, V., Štajner, T., Đokić, V., Klun, I., Bobić, B., Nikolić, A.,& Đurković-Đaković, O.. (2014). Evidence for genetic diversity of Toxoplasma gondii in selected intermediate hosts in Serbia. in Comparative Immunology Microbiology & Infectious Diseases Elsevier Sci Ltd, Oxford., 37(3), 173-179. https://doi.org/10.1016/j.cimid.2014.03.001
Marković M, Ivović V, Štajner T, Đokić V, Klun I, Bobić B, Nikolić A, Đurković-Đaković O. Evidence for genetic diversity of Toxoplasma gondii in selected intermediate hosts in Serbia. in Comparative Immunology Microbiology & Infectious Diseases. 2014;37(3):173-179. doi:10.1016/j.cimid.2014.03.001 .
Marković, Marija, Ivović, Vladimir, Štajner, Tijana, Đokić, Vitomir, Klun, Ivana, Bobić, Branko, Nikolić, Aleksandra, Đurković-Đaković, Olgica, "Evidence for genetic diversity of Toxoplasma gondii in selected intermediate hosts in Serbia" in Comparative Immunology Microbiology & Infectious Diseases, 37, no. 3 (2014):173-179, https://doi.org/10.1016/j.cimid.2014.03.001 . .