Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging
2014
Authors
Popović, Tamara B.Borozan, Sunčica
Takić, Marija
Kojadinović, Milica
Ranković, Slavica G.
Ranić, Marija M.
De Luka, Silvio R.
Article (Published version)
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Fish oil affects oxidative stress parameters and changes in phospholipids fatty acids profiles in plasma, erythrocytes and tissues. We examined the effects of fish oil supplementation in young and old male Wistar rats (3 and 22 months old) on plasma phospholipids fatty acids profiles and blood oxidative stress parameters. Twenty young and twenty aged Wistar rats were randomly divided into four groups (ten animals each): two control groups and two supplemented groups treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid (EPA) and 30 mg docosahexanoic acid (DHA). Fish oil supplementation changed the percentage of long chain fatty acids (FAs): the elevated percentage of eicosatrienoic acid (ETA, 20:3), eicosapentanoic (EPA, 20:5), docosapentanoic acid (DPA, 22:5), n-3 fatty acids and decreased arachidonic acid (AA, 20:4). However, there were no age-related changes in total SH groups, the percentages of palmitic (16:0), palmitoleic (16:1), oleic (18:1 (n-9)) and ...linoleic acid (18:2) in plasma phospholipids and MUFA and they were neither reversed nor prevented by fish oil supplementation. Results showed that fish oil supplementation increased SOD and CAT activities in erythrocytes, and PON activity in the blood plasma of both young and aged rats. Furthermore, fish oil supplementation decreased lipid peroxidation (MDA) and nitrite levels in both young and aged rats implying better antioxidant protection and a lower level of oxidative pressure after fish oil supplementation. Our results suggest that fish oil supplementation is beneficial regarding better antioxidant protection in both young and aged rats, while applied treatment differs in plasma phospholipids FAs composition.
Keywords:
fish oil / aged rats / oxidative stress / fatty acidsSource:
Croatica Chemica Acta, 2014, 87, 3, 207-212Publisher:
- Croatian Chemical Soc, Zagreb
Funding / projects:
- Biological effects, nutritional intake and status of folate and polysaturate fatty acid (PUFA): improvement of nutrition in Serbia (RS-41030)
- Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell (RS-173034)
DOI: 10.5562/cca2405
ISSN: 0011-1643
WoS: 000348414000003
Scopus: 2-s2.0-84919709714
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Institut za medicinska istraživanjaTY - JOUR AU - Popović, Tamara B. AU - Borozan, Sunčica AU - Takić, Marija AU - Kojadinović, Milica AU - Ranković, Slavica G. AU - Ranić, Marija M. AU - De Luka, Silvio R. PY - 2014 UR - http://rimi.imi.bg.ac.rs/handle/123456789/538 AB - Fish oil affects oxidative stress parameters and changes in phospholipids fatty acids profiles in plasma, erythrocytes and tissues. We examined the effects of fish oil supplementation in young and old male Wistar rats (3 and 22 months old) on plasma phospholipids fatty acids profiles and blood oxidative stress parameters. Twenty young and twenty aged Wistar rats were randomly divided into four groups (ten animals each): two control groups and two supplemented groups treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid (EPA) and 30 mg docosahexanoic acid (DHA). Fish oil supplementation changed the percentage of long chain fatty acids (FAs): the elevated percentage of eicosatrienoic acid (ETA, 20:3), eicosapentanoic (EPA, 20:5), docosapentanoic acid (DPA, 22:5), n-3 fatty acids and decreased arachidonic acid (AA, 20:4). However, there were no age-related changes in total SH groups, the percentages of palmitic (16:0), palmitoleic (16:1), oleic (18:1 (n-9)) and linoleic acid (18:2) in plasma phospholipids and MUFA and they were neither reversed nor prevented by fish oil supplementation. Results showed that fish oil supplementation increased SOD and CAT activities in erythrocytes, and PON activity in the blood plasma of both young and aged rats. Furthermore, fish oil supplementation decreased lipid peroxidation (MDA) and nitrite levels in both young and aged rats implying better antioxidant protection and a lower level of oxidative pressure after fish oil supplementation. Our results suggest that fish oil supplementation is beneficial regarding better antioxidant protection in both young and aged rats, while applied treatment differs in plasma phospholipids FAs composition. PB - Croatian Chemical Soc, Zagreb T2 - Croatica Chemica Acta T1 - Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging EP - 212 IS - 3 SP - 207 VL - 87 DO - 10.5562/cca2405 ER -
@article{ author = "Popović, Tamara B. and Borozan, Sunčica and Takić, Marija and Kojadinović, Milica and Ranković, Slavica G. and Ranić, Marija M. and De Luka, Silvio R.", year = "2014", abstract = "Fish oil affects oxidative stress parameters and changes in phospholipids fatty acids profiles in plasma, erythrocytes and tissues. We examined the effects of fish oil supplementation in young and old male Wistar rats (3 and 22 months old) on plasma phospholipids fatty acids profiles and blood oxidative stress parameters. Twenty young and twenty aged Wistar rats were randomly divided into four groups (ten animals each): two control groups and two supplemented groups treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid (EPA) and 30 mg docosahexanoic acid (DHA). Fish oil supplementation changed the percentage of long chain fatty acids (FAs): the elevated percentage of eicosatrienoic acid (ETA, 20:3), eicosapentanoic (EPA, 20:5), docosapentanoic acid (DPA, 22:5), n-3 fatty acids and decreased arachidonic acid (AA, 20:4). However, there were no age-related changes in total SH groups, the percentages of palmitic (16:0), palmitoleic (16:1), oleic (18:1 (n-9)) and linoleic acid (18:2) in plasma phospholipids and MUFA and they were neither reversed nor prevented by fish oil supplementation. Results showed that fish oil supplementation increased SOD and CAT activities in erythrocytes, and PON activity in the blood plasma of both young and aged rats. Furthermore, fish oil supplementation decreased lipid peroxidation (MDA) and nitrite levels in both young and aged rats implying better antioxidant protection and a lower level of oxidative pressure after fish oil supplementation. Our results suggest that fish oil supplementation is beneficial regarding better antioxidant protection in both young and aged rats, while applied treatment differs in plasma phospholipids FAs composition.", publisher = "Croatian Chemical Soc, Zagreb", journal = "Croatica Chemica Acta", title = "Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging", pages = "212-207", number = "3", volume = "87", doi = "10.5562/cca2405" }
Popović, T. B., Borozan, S., Takić, M., Kojadinović, M., Ranković, S. G., Ranić, M. M.,& De Luka, S. R.. (2014). Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging. in Croatica Chemica Acta Croatian Chemical Soc, Zagreb., 87(3), 207-212. https://doi.org/10.5562/cca2405
Popović TB, Borozan S, Takić M, Kojadinović M, Ranković SG, Ranić MM, De Luka SR. Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging. in Croatica Chemica Acta. 2014;87(3):207-212. doi:10.5562/cca2405 .
Popović, Tamara B., Borozan, Sunčica, Takić, Marija, Kojadinović, Milica, Ranković, Slavica G., Ranić, Marija M., De Luka, Silvio R., "Fatty Acid Composition and Oxidative Stress Parameters in Plasma after Fish Oil Supplementation in Aging" in Croatica Chemica Acta, 87, no. 3 (2014):207-212, https://doi.org/10.5562/cca2405 . .