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Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA
dc.creator | Budeč, Mirela | |
dc.creator | Marković, Dragana | |
dc.creator | Vignjević, Sanja | |
dc.creator | Mitrović, Olivera | |
dc.creator | Đikić, Dragoslava | |
dc.creator | Koko, Vesna | |
dc.creator | Čokić, Vladan | |
dc.date.accessioned | 2021-04-20T12:36:16Z | |
dc.date.available | 2021-04-20T12:36:16Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 0735-0414 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/523 | |
dc.description.abstract | Aims: We showed previously that the acute effect of ethanol on intestinal immunoglobulin A (IgA) expression might be mediated by endogenous nitric oxide (NO). To extend these findings, this study was designed to investigate a possible role of neuronal NO synthase (nNOS) in the observed phenomenon, using 7-nitroindazole (7-NI), a selective inhibitor of its activity. Methods: Adult male Wistar rats were treated with: (a) ethanol (4 g/kg, intraperitoneally, i.p.), (b) 7-NI (25 mg/kg, i.p.) followed by ethanol (4 g/kg, i.p.) 30 min later and (c) 7-NI (25 mg/kg, i.p.) followed by saline 30 min later. Untreated rats were used as controls. The concentrations of serum and intestinal IgA were measured by enzyme-linked immunosorbent assay, while the expression of nNOS was determined using western blot and immunohistochemistry. Results: Acute ethanol treatment significantly increased the concentration of IgA in the ileal extracts, whereas it decreased its serum level. Inhibition of nNOS activity by 7-NI abolished this action of alcohol on IgA. Additionally, western blot analysis revealed that the acute alcohol administration induced an increase in the expression of intestinal nNOS. Furthermore, nNOS-immunoreactive cells, observed within the lamina propria of small intestine, were numerous in ethanol-treated rats. Conclusion: Taken together, these results extended our previous findings suggesting that nNOS mediates the acute effect of ethanol on IgA and supported an immunomodulatory role of this enzyme isoform. | en |
dc.publisher | Oxford Univ Press, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175053/RS// | |
dc.rights | openAccess | |
dc.source | Alcohol & Alcoholism | |
dc.title | Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 58 | |
dc.citation.issue | 1 | |
dc.citation.other | 48(1): 53-58 | |
dc.citation.rank | M21 | |
dc.citation.spage | 53 | |
dc.citation.volume | 48 | |
dc.identifier.doi | 10.1093/alcalc/ags110 | |
dc.identifier.fulltext | http://rimi.imi.bg.ac.rs/bitstream/id/397/520.pdf | |
dc.identifier.pmid | 23059423 | |
dc.identifier.scopus | 2-s2.0-84871591877 | |
dc.identifier.wos | 000312652300007 | |
dc.type.version | publishedVersion |