Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA
2013
Autori
Budeč, MirelaMarković, Dragana
Vignjević, Sanja
Mitrović, Olivera
Đikić, Dragoslava
Koko, Vesna
Čokić, Vladan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Aims: We showed previously that the acute effect of ethanol on intestinal immunoglobulin A (IgA) expression might be mediated by endogenous nitric oxide (NO). To extend these findings, this study was designed to investigate a possible role of neuronal NO synthase (nNOS) in the observed phenomenon, using 7-nitroindazole (7-NI), a selective inhibitor of its activity. Methods: Adult male Wistar rats were treated with: (a) ethanol (4 g/kg, intraperitoneally, i.p.), (b) 7-NI (25 mg/kg, i.p.) followed by ethanol (4 g/kg, i.p.) 30 min later and (c) 7-NI (25 mg/kg, i.p.) followed by saline 30 min later. Untreated rats were used as controls. The concentrations of serum and intestinal IgA were measured by enzyme-linked immunosorbent assay, while the expression of nNOS was determined using western blot and immunohistochemistry. Results: Acute ethanol treatment significantly increased the concentration of IgA in the ileal extracts, whereas it decreased its serum level. Inhibition of nNOS activity ...by 7-NI abolished this action of alcohol on IgA. Additionally, western blot analysis revealed that the acute alcohol administration induced an increase in the expression of intestinal nNOS. Furthermore, nNOS-immunoreactive cells, observed within the lamina propria of small intestine, were numerous in ethanol-treated rats. Conclusion: Taken together, these results extended our previous findings suggesting that nNOS mediates the acute effect of ethanol on IgA and supported an immunomodulatory role of this enzyme isoform.
Izvor:
Alcohol & Alcoholism, 2013, 48, 1, 53-58Izdavač:
- Oxford Univ Press, Oxford
Finansiranje / projekti:
- Ispitivanje patogeneze hematoloških maligniteta (RS-MESTD-Basic Research (BR or ON)-175053)
DOI: 10.1093/alcalc/ags110
ISSN: 0735-0414
PubMed: 23059423
WoS: 000312652300007
Scopus: 2-s2.0-84871591877
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Budeč, Mirela AU - Marković, Dragana AU - Vignjević, Sanja AU - Mitrović, Olivera AU - Đikić, Dragoslava AU - Koko, Vesna AU - Čokić, Vladan PY - 2013 UR - http://rimi.imi.bg.ac.rs/handle/123456789/523 AB - Aims: We showed previously that the acute effect of ethanol on intestinal immunoglobulin A (IgA) expression might be mediated by endogenous nitric oxide (NO). To extend these findings, this study was designed to investigate a possible role of neuronal NO synthase (nNOS) in the observed phenomenon, using 7-nitroindazole (7-NI), a selective inhibitor of its activity. Methods: Adult male Wistar rats were treated with: (a) ethanol (4 g/kg, intraperitoneally, i.p.), (b) 7-NI (25 mg/kg, i.p.) followed by ethanol (4 g/kg, i.p.) 30 min later and (c) 7-NI (25 mg/kg, i.p.) followed by saline 30 min later. Untreated rats were used as controls. The concentrations of serum and intestinal IgA were measured by enzyme-linked immunosorbent assay, while the expression of nNOS was determined using western blot and immunohistochemistry. Results: Acute ethanol treatment significantly increased the concentration of IgA in the ileal extracts, whereas it decreased its serum level. Inhibition of nNOS activity by 7-NI abolished this action of alcohol on IgA. Additionally, western blot analysis revealed that the acute alcohol administration induced an increase in the expression of intestinal nNOS. Furthermore, nNOS-immunoreactive cells, observed within the lamina propria of small intestine, were numerous in ethanol-treated rats. Conclusion: Taken together, these results extended our previous findings suggesting that nNOS mediates the acute effect of ethanol on IgA and supported an immunomodulatory role of this enzyme isoform. PB - Oxford Univ Press, Oxford T2 - Alcohol & Alcoholism T1 - Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA EP - 58 IS - 1 SP - 53 VL - 48 DO - 10.1093/alcalc/ags110 ER -
@article{ author = "Budeč, Mirela and Marković, Dragana and Vignjević, Sanja and Mitrović, Olivera and Đikić, Dragoslava and Koko, Vesna and Čokić, Vladan", year = "2013", abstract = "Aims: We showed previously that the acute effect of ethanol on intestinal immunoglobulin A (IgA) expression might be mediated by endogenous nitric oxide (NO). To extend these findings, this study was designed to investigate a possible role of neuronal NO synthase (nNOS) in the observed phenomenon, using 7-nitroindazole (7-NI), a selective inhibitor of its activity. Methods: Adult male Wistar rats were treated with: (a) ethanol (4 g/kg, intraperitoneally, i.p.), (b) 7-NI (25 mg/kg, i.p.) followed by ethanol (4 g/kg, i.p.) 30 min later and (c) 7-NI (25 mg/kg, i.p.) followed by saline 30 min later. Untreated rats were used as controls. The concentrations of serum and intestinal IgA were measured by enzyme-linked immunosorbent assay, while the expression of nNOS was determined using western blot and immunohistochemistry. Results: Acute ethanol treatment significantly increased the concentration of IgA in the ileal extracts, whereas it decreased its serum level. Inhibition of nNOS activity by 7-NI abolished this action of alcohol on IgA. Additionally, western blot analysis revealed that the acute alcohol administration induced an increase in the expression of intestinal nNOS. Furthermore, nNOS-immunoreactive cells, observed within the lamina propria of small intestine, were numerous in ethanol-treated rats. Conclusion: Taken together, these results extended our previous findings suggesting that nNOS mediates the acute effect of ethanol on IgA and supported an immunomodulatory role of this enzyme isoform.", publisher = "Oxford Univ Press, Oxford", journal = "Alcohol & Alcoholism", title = "Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA", pages = "58-53", number = "1", volume = "48", doi = "10.1093/alcalc/ags110" }
Budeč, M., Marković, D., Vignjević, S., Mitrović, O., Đikić, D., Koko, V.,& Čokić, V.. (2013). Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA. in Alcohol & Alcoholism Oxford Univ Press, Oxford., 48(1), 53-58. https://doi.org/10.1093/alcalc/ags110
Budeč M, Marković D, Vignjević S, Mitrović O, Đikić D, Koko V, Čokić V. Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA. in Alcohol & Alcoholism. 2013;48(1):53-58. doi:10.1093/alcalc/ags110 .
Budeč, Mirela, Marković, Dragana, Vignjević, Sanja, Mitrović, Olivera, Đikić, Dragoslava, Koko, Vesna, Čokić, Vladan, "Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA" in Alcohol & Alcoholism, 48, no. 1 (2013):53-58, https://doi.org/10.1093/alcalc/ags110 . .