In vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levels
Само за регистроване кориснике
2013
Аутори
Krstić, JelenaJauković, Aleksandra
Mojsilović, Slavko
Okić Đorđević, Ivana
Trivanović, Drenka
Ilić, Vesna
Santibanez, Juan F.
Bugarski, Diana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study has been to elucidate how different oxygen levels impact the effects of Interleukin-17 (IL-17) on angiogenic properties of endothelial cells. Two endothelial cell lines, mouse MS-1 and human EA.hy 926, were grown in 20% and 3% O-2 and their angiogenic abilities analyzed after IL-17 treatment: proliferation, apoptosis, migration and tubulogenesis. Expression of endothelial nitric oxide synthase (eNOS) and cyclooxygenase-2 (Cox-2) was also measured. Considering EA.hy 926 cell line, hypoxia alone reduced proliferation, survival and migration, but not their ability to form tubules. When cultured at 20% O-2, IL-17 stimulated proliferation, migration and tubulogenesis, whereas a hypoxic environment did not affect their migration and proliferation, but increased their survival and tubulogenic properties. Expression of eNOS and Cox-2 increased by both IL-17 and hypoxia, as well as with their combination. With the MS-1 cell line hypoxia did not affect proliferation, surviv...al, migration and tubule formation. At 20% O-2, IL-17 did not alter their proliferation,but inhibited migration and stimulated tubule formation. At 3% O-2, only the stimulating effect of IL-17 on tubulogenesis was evident. The constitutive expression of eNOS was unaffected by oxygen concentrations or IL-17 supplementation, whereas both IL-17 and hypoxia upregulated Cox-2 expression. Thus the effects of IL-17 on the angiogenic properties of endothelial cells depend on both the cell line used and the oxygen concentration.
Кључне речи:
angiogenesis / endothelial cells / EA / hy 926 / hypoxia / IL-17 / MS-1Извор:
Cell Biology International, 2013, 37, 11, 1162-1170Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-MESTD-Basic Research (BR or ON)-175062)
DOI: 10.1002/cbin.10144
ISSN: 1065-6995
PubMed: 23765637
WoS: 000325489500004
Scopus: 2-s2.0-84885868031
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Krstić, Jelena AU - Jauković, Aleksandra AU - Mojsilović, Slavko AU - Okić Đorđević, Ivana AU - Trivanović, Drenka AU - Ilić, Vesna AU - Santibanez, Juan F. AU - Bugarski, Diana PY - 2013 UR - http://rimi.imi.bg.ac.rs/handle/123456789/484 AB - The aim of this study has been to elucidate how different oxygen levels impact the effects of Interleukin-17 (IL-17) on angiogenic properties of endothelial cells. Two endothelial cell lines, mouse MS-1 and human EA.hy 926, were grown in 20% and 3% O-2 and their angiogenic abilities analyzed after IL-17 treatment: proliferation, apoptosis, migration and tubulogenesis. Expression of endothelial nitric oxide synthase (eNOS) and cyclooxygenase-2 (Cox-2) was also measured. Considering EA.hy 926 cell line, hypoxia alone reduced proliferation, survival and migration, but not their ability to form tubules. When cultured at 20% O-2, IL-17 stimulated proliferation, migration and tubulogenesis, whereas a hypoxic environment did not affect their migration and proliferation, but increased their survival and tubulogenic properties. Expression of eNOS and Cox-2 increased by both IL-17 and hypoxia, as well as with their combination. With the MS-1 cell line hypoxia did not affect proliferation, survival, migration and tubule formation. At 20% O-2, IL-17 did not alter their proliferation,but inhibited migration and stimulated tubule formation. At 3% O-2, only the stimulating effect of IL-17 on tubulogenesis was evident. The constitutive expression of eNOS was unaffected by oxygen concentrations or IL-17 supplementation, whereas both IL-17 and hypoxia upregulated Cox-2 expression. Thus the effects of IL-17 on the angiogenic properties of endothelial cells depend on both the cell line used and the oxygen concentration. PB - Wiley, Hoboken T2 - Cell Biology International T1 - In vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levels EP - 1170 IS - 11 SP - 1162 VL - 37 DO - 10.1002/cbin.10144 ER -
@article{ author = "Krstić, Jelena and Jauković, Aleksandra and Mojsilović, Slavko and Okić Đorđević, Ivana and Trivanović, Drenka and Ilić, Vesna and Santibanez, Juan F. and Bugarski, Diana", year = "2013", abstract = "The aim of this study has been to elucidate how different oxygen levels impact the effects of Interleukin-17 (IL-17) on angiogenic properties of endothelial cells. Two endothelial cell lines, mouse MS-1 and human EA.hy 926, were grown in 20% and 3% O-2 and their angiogenic abilities analyzed after IL-17 treatment: proliferation, apoptosis, migration and tubulogenesis. Expression of endothelial nitric oxide synthase (eNOS) and cyclooxygenase-2 (Cox-2) was also measured. Considering EA.hy 926 cell line, hypoxia alone reduced proliferation, survival and migration, but not their ability to form tubules. When cultured at 20% O-2, IL-17 stimulated proliferation, migration and tubulogenesis, whereas a hypoxic environment did not affect their migration and proliferation, but increased their survival and tubulogenic properties. Expression of eNOS and Cox-2 increased by both IL-17 and hypoxia, as well as with their combination. With the MS-1 cell line hypoxia did not affect proliferation, survival, migration and tubule formation. At 20% O-2, IL-17 did not alter their proliferation,but inhibited migration and stimulated tubule formation. At 3% O-2, only the stimulating effect of IL-17 on tubulogenesis was evident. The constitutive expression of eNOS was unaffected by oxygen concentrations or IL-17 supplementation, whereas both IL-17 and hypoxia upregulated Cox-2 expression. Thus the effects of IL-17 on the angiogenic properties of endothelial cells depend on both the cell line used and the oxygen concentration.", publisher = "Wiley, Hoboken", journal = "Cell Biology International", title = "In vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levels", pages = "1170-1162", number = "11", volume = "37", doi = "10.1002/cbin.10144" }
Krstić, J., Jauković, A., Mojsilović, S., Okić Đorđević, I., Trivanović, D., Ilić, V., Santibanez, J. F.,& Bugarski, D.. (2013). In vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levels. in Cell Biology International Wiley, Hoboken., 37(11), 1162-1170. https://doi.org/10.1002/cbin.10144
Krstić J, Jauković A, Mojsilović S, Okić Đorđević I, Trivanović D, Ilić V, Santibanez JF, Bugarski D. In vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levels. in Cell Biology International. 2013;37(11):1162-1170. doi:10.1002/cbin.10144 .
Krstić, Jelena, Jauković, Aleksandra, Mojsilović, Slavko, Okić Đorđević, Ivana, Trivanović, Drenka, Ilić, Vesna, Santibanez, Juan F., Bugarski, Diana, "In vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levels" in Cell Biology International, 37, no. 11 (2013):1162-1170, https://doi.org/10.1002/cbin.10144 . .