Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis
Само за регистроване кориснике
2012
Аутори
Kovačević-Filipović, MilicaIlić, Vesna
Vujcić, Zoran
Dojnov, Biljana
Stevanov-Pavlović, Marija
Mijacević, Zora
Božić, Tatjana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows... in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content.
Кључне речи:
Serum amyloid A / Isoforms / Subclinical mastitis / Staphylococcus aureusИзвор:
Veterinary Immunology & Immunopathology, 2012, 145, 1-2, 120-128Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Антиоксидативна заштита и потенцијали за диференцијацију и регенерацију мезенхималних матичних ћелија из различитих ткива током процеса старења (RS-175061)
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-175062)
- Производња, изоловање и карактеризација ензима и малих молекула и њихова примена у растворном и имобилизованом облику у биотехнологији хране, биогоривима и заштитити животне средине (RS-172048)
- Унапређење и развој хигијенских и технолошких поступака у производњи намирница животињског порекла у циљу добијања квалитетних и безбедних производа конкурентних на светском тржишту (RS-46009)
DOI: 10.1016/j.vetimm.2011.10.015
ISSN: 0165-2427
PubMed: 22100192
WoS: 000301159200013
Scopus: 2-s2.0-84856555615
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Kovačević-Filipović, Milica AU - Ilić, Vesna AU - Vujcić, Zoran AU - Dojnov, Biljana AU - Stevanov-Pavlović, Marija AU - Mijacević, Zora AU - Božić, Tatjana PY - 2012 UR - http://rimi.imi.bg.ac.rs/handle/123456789/447 AB - Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content. PB - Elsevier, Amsterdam T2 - Veterinary Immunology & Immunopathology T1 - Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis EP - 128 IS - 1-2 SP - 120 VL - 145 DO - 10.1016/j.vetimm.2011.10.015 ER -
@article{ author = "Kovačević-Filipović, Milica and Ilić, Vesna and Vujcić, Zoran and Dojnov, Biljana and Stevanov-Pavlović, Marija and Mijacević, Zora and Božić, Tatjana", year = "2012", abstract = "Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content.", publisher = "Elsevier, Amsterdam", journal = "Veterinary Immunology & Immunopathology", title = "Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis", pages = "128-120", number = "1-2", volume = "145", doi = "10.1016/j.vetimm.2011.10.015" }
Kovačević-Filipović, M., Ilić, V., Vujcić, Z., Dojnov, B., Stevanov-Pavlović, M., Mijacević, Z.,& Božić, T.. (2012). Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis. in Veterinary Immunology & Immunopathology Elsevier, Amsterdam., 145(1-2), 120-128. https://doi.org/10.1016/j.vetimm.2011.10.015
Kovačević-Filipović M, Ilić V, Vujcić Z, Dojnov B, Stevanov-Pavlović M, Mijacević Z, Božić T. Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis. in Veterinary Immunology & Immunopathology. 2012;145(1-2):120-128. doi:10.1016/j.vetimm.2011.10.015 .
Kovačević-Filipović, Milica, Ilić, Vesna, Vujcić, Zoran, Dojnov, Biljana, Stevanov-Pavlović, Marija, Mijacević, Zora, Božić, Tatjana, "Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis" in Veterinary Immunology & Immunopathology, 145, no. 1-2 (2012):120-128, https://doi.org/10.1016/j.vetimm.2011.10.015 . .