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SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes
dc.creator | Kocić, Jelena | |
dc.creator | Villar, Victor | |
dc.creator | Krstić, Aleksandra | |
dc.creator | Santibanez, Juan F. | |
dc.creator | Kocić, Jelena | |
dc.creator | Villar, Victor | |
dc.creator | Krstić, Aleksandra | |
dc.creator | Santibanez, Juan F. | |
dc.date.accessioned | 2021-04-20T12:30:00Z | |
dc.date.available | 2021-04-20T12:30:00Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 2090-908X | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/427 | |
dc.description.abstract | Transforming growth factor-beta (TGF-β1) is a potent inductor of matrix metalloproteinase-9 (MMP-9) in transformed cells. Recently, Ski-interacting protein (SKIP) has been described as a regulator of TGF-β1 signal transduction, but its role in the induction of cell malignance by TGF-β1 has not been fully elucidated so far. In the present study, we analyzed the role of SKIP on TGF-β1-induced MMP-9 production. Mouse transformed keratinocytes (PDV) were stably transfected with SKIP antisense construct. We observed that SKIP depletion provoked an enhancement in the expression of MMP-9 in response to TGF-β1 treatment. The downregulation of SKIP produced an enhancement in TGF-β1-activated ERK1,2 MAP kinase as well as increased transactivation of downstream Elk1 transcription factor. The increased MMP-9 production in response to TGF-β1 was dependent of MAPK activation as PD98059, an MEK inhibitor, reduced MMP-9 expression in SKIP antisense transfected cells. Thus, we propose SKIP as a regulatory protein in TGF-β1-induced MMP-9 expression acting by controlling ERK1,2 signaling in transformed cells. | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS// | |
dc.rights | restrictedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Scientifica | |
dc.subject | keratinocytes | |
dc.subject | SKIP downregulation | |
dc.subject | TGF-β1 | |
dc.subject | MMP-9 | |
dc.title | SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes | |
dc.type | article | |
dc.rights.license | BY | |
dc.citation.other | 2012: - | |
dc.citation.volume | 2012 | |
dc.identifier.doi | 10.6064/2012/861647 | |
dc.type.version | publishedVersion |