SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes
Само за регистроване кориснике
2012
Аутори
Kocić, JelenaVillar, Victor
Krstić, Aleksandra
Santibanez, Juan F.
Kocić, Jelena
Villar, Victor
Krstić, Aleksandra
Santibanez, Juan F.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Transforming growth factor-beta (TGF-β1) is a potent inductor of matrix metalloproteinase-9 (MMP-9) in transformed cells. Recently, Ski-interacting protein (SKIP) has been described as a regulator of TGF-β1 signal transduction, but its role in the induction of cell malignance by TGF-β1 has not been fully elucidated so far. In the present study, we analyzed the role of SKIP on TGF-β1-induced MMP-9 production. Mouse transformed keratinocytes (PDV) were stably transfected with SKIP antisense construct. We observed that SKIP depletion provoked an enhancement in the expression of MMP-9 in response to TGF-β1 treatment. The downregulation of SKIP produced an enhancement in TGF-β1-activated ERK1,2 MAP kinase as well as increased transactivation of downstream Elk1 transcription factor. The increased MMP-9 production in response to TGF-β1 was dependent of MAPK activation as PD98059, an MEK inhibitor, reduced MMP-9 expression in SKIP antisense transfected cells. Thus, we propose SKIP as a regulat...ory protein in TGF-β1-induced MMP-9 expression acting by controlling ERK1,2 signaling in transformed cells.
Кључне речи:
keratinocytes / SKIP downregulation / TGF-β1 / MMP-9Извор:
Scientifica, 2012, 2012Финансирање / пројекти:
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-MESTD-Basic Research (BR or ON)-175062)
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Kocić, Jelena AU - Villar, Victor AU - Krstić, Aleksandra AU - Santibanez, Juan F. AU - Kocić, Jelena AU - Villar, Victor AU - Krstić, Aleksandra AU - Santibanez, Juan F. PY - 2012 UR - http://rimi.imi.bg.ac.rs/handle/123456789/427 AB - Transforming growth factor-beta (TGF-β1) is a potent inductor of matrix metalloproteinase-9 (MMP-9) in transformed cells. Recently, Ski-interacting protein (SKIP) has been described as a regulator of TGF-β1 signal transduction, but its role in the induction of cell malignance by TGF-β1 has not been fully elucidated so far. In the present study, we analyzed the role of SKIP on TGF-β1-induced MMP-9 production. Mouse transformed keratinocytes (PDV) were stably transfected with SKIP antisense construct. We observed that SKIP depletion provoked an enhancement in the expression of MMP-9 in response to TGF-β1 treatment. The downregulation of SKIP produced an enhancement in TGF-β1-activated ERK1,2 MAP kinase as well as increased transactivation of downstream Elk1 transcription factor. The increased MMP-9 production in response to TGF-β1 was dependent of MAPK activation as PD98059, an MEK inhibitor, reduced MMP-9 expression in SKIP antisense transfected cells. Thus, we propose SKIP as a regulatory protein in TGF-β1-induced MMP-9 expression acting by controlling ERK1,2 signaling in transformed cells. T2 - Scientifica T1 - SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes VL - 2012 DO - 10.6064/2012/861647 ER -
@article{ author = "Kocić, Jelena and Villar, Victor and Krstić, Aleksandra and Santibanez, Juan F. and Kocić, Jelena and Villar, Victor and Krstić, Aleksandra and Santibanez, Juan F.", year = "2012", abstract = "Transforming growth factor-beta (TGF-β1) is a potent inductor of matrix metalloproteinase-9 (MMP-9) in transformed cells. Recently, Ski-interacting protein (SKIP) has been described as a regulator of TGF-β1 signal transduction, but its role in the induction of cell malignance by TGF-β1 has not been fully elucidated so far. In the present study, we analyzed the role of SKIP on TGF-β1-induced MMP-9 production. Mouse transformed keratinocytes (PDV) were stably transfected with SKIP antisense construct. We observed that SKIP depletion provoked an enhancement in the expression of MMP-9 in response to TGF-β1 treatment. The downregulation of SKIP produced an enhancement in TGF-β1-activated ERK1,2 MAP kinase as well as increased transactivation of downstream Elk1 transcription factor. The increased MMP-9 production in response to TGF-β1 was dependent of MAPK activation as PD98059, an MEK inhibitor, reduced MMP-9 expression in SKIP antisense transfected cells. Thus, we propose SKIP as a regulatory protein in TGF-β1-induced MMP-9 expression acting by controlling ERK1,2 signaling in transformed cells.", journal = "Scientifica", title = "SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes", volume = "2012", doi = "10.6064/2012/861647" }
Kocić, J., Villar, V., Krstić, A., Santibanez, J. F., Kocić, J., Villar, V., Krstić, A.,& Santibanez, J. F.. (2012). SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes. in Scientifica, 2012. https://doi.org/10.6064/2012/861647
Kocić J, Villar V, Krstić A, Santibanez JF, Kocić J, Villar V, Krstić A, Santibanez JF. SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes. in Scientifica. 2012;2012. doi:10.6064/2012/861647 .
Kocić, Jelena, Villar, Victor, Krstić, Aleksandra, Santibanez, Juan F., Kocić, Jelena, Villar, Victor, Krstić, Aleksandra, Santibanez, Juan F., "SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes" in Scientifica, 2012 (2012), https://doi.org/10.6064/2012/861647 . .