Similar Developmental Patterns of Ghrelin- and Glucagon-Expressing Cells in the Human Pancreas
Само за регистроване кориснике
2012
Аутори
Vignjević, SanjaTodorović, Vera
Damjanović, Svetozar
Budeč, Mirela
Mitrović, Olivera
Đikić, Dragoslava
Drndarević, Neda
Mićić, Mileva
Miskovic-Krivokapić, Jelena
Đuricić, Slavisa
Nikolić, Ivan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The pancreas appears to be a major source of ghrelin during fetal development, but the ontogeny of ghrelin cells in the human pancreas and their developmental relationship with alpha- and beta-cells remain largely unknown. In the present study, we examined the dynamics of ghrelin cell growth, colocalization of ghrelin with major pancreatic hormones and defined the similarities and differences among developmental patterns of ghrelin-, glucagon- and insulin-expressing cells in the human pancreas. To this end, paraffin-embedded pancreatic tissue sections from human embryos and fetuses were assessed by immunohistochemistry. Ghrelin-positive cells were first detected in the pancreas of 11-week-old fetuses. With advancing gestational age, both ghrelin- and glucagon-expressing cells were increasingly observed at the periphery of the developing islets, whereas insulin-containing cells were typically found in the islet core. Double immunohistochemistry showed that ghrelin-expressing cells were ...clearly separate from insulin-, somatostatin- and pancreatic polypeptide-containing cells. In contrast, cells coexpressing ghrelin and glucagon were sporadically detected during both the early and late fetal periods. Furthermore, morphometric analysis revealed a similar trend in the volume density of ghrelin- and glucagon-positive cells, and a contrasting pattern in beta-cell density at specific time points during the development of the human pancreas. This study demonstrates that the developmental pattern of ghrelin cells, although clearly distinct, is quite similar to that of glucagon-expressing cells. The obtained findings indicate a close lineage relationship between these cell populations, a functional relationship between their secretory products and an auto/paracrine mode of ghrelin-glucagon interaction in pancreatic development. Copyright (C) 2012 S. Karger AG, Basel
Кључне речи:
Development / Ghrelin / Glucagon / Insulin / PancreasИзвор:
Cells Tissues Organs, 2012, 196, 4, 362-373Издавач:
- Karger, Basel
Финансирање / пројекти:
- Испитивање патогенезе хематолошких малигнитета (RS-MESTD-Basic Research (BR or ON)-175053)
- Антиоксидативна заштита и потенцијали за диференцијацију и регенерацију мезенхималних матичних ћелија из различитих ткива током процеса старења (RS-MESTD-Basic Research (BR or ON)-175061)
DOI: 10.1159/000335469
ISSN: 1422-6405
PubMed: 22538872
WoS: 000309035500006
Scopus: 2-s2.0-84866925470
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Vignjević, Sanja AU - Todorović, Vera AU - Damjanović, Svetozar AU - Budeč, Mirela AU - Mitrović, Olivera AU - Đikić, Dragoslava AU - Drndarević, Neda AU - Mićić, Mileva AU - Miskovic-Krivokapić, Jelena AU - Đuricić, Slavisa AU - Nikolić, Ivan PY - 2012 UR - http://rimi.imi.bg.ac.rs/handle/123456789/419 AB - The pancreas appears to be a major source of ghrelin during fetal development, but the ontogeny of ghrelin cells in the human pancreas and their developmental relationship with alpha- and beta-cells remain largely unknown. In the present study, we examined the dynamics of ghrelin cell growth, colocalization of ghrelin with major pancreatic hormones and defined the similarities and differences among developmental patterns of ghrelin-, glucagon- and insulin-expressing cells in the human pancreas. To this end, paraffin-embedded pancreatic tissue sections from human embryos and fetuses were assessed by immunohistochemistry. Ghrelin-positive cells were first detected in the pancreas of 11-week-old fetuses. With advancing gestational age, both ghrelin- and glucagon-expressing cells were increasingly observed at the periphery of the developing islets, whereas insulin-containing cells were typically found in the islet core. Double immunohistochemistry showed that ghrelin-expressing cells were clearly separate from insulin-, somatostatin- and pancreatic polypeptide-containing cells. In contrast, cells coexpressing ghrelin and glucagon were sporadically detected during both the early and late fetal periods. Furthermore, morphometric analysis revealed a similar trend in the volume density of ghrelin- and glucagon-positive cells, and a contrasting pattern in beta-cell density at specific time points during the development of the human pancreas. This study demonstrates that the developmental pattern of ghrelin cells, although clearly distinct, is quite similar to that of glucagon-expressing cells. The obtained findings indicate a close lineage relationship between these cell populations, a functional relationship between their secretory products and an auto/paracrine mode of ghrelin-glucagon interaction in pancreatic development. Copyright (C) 2012 S. Karger AG, Basel PB - Karger, Basel T2 - Cells Tissues Organs T1 - Similar Developmental Patterns of Ghrelin- and Glucagon-Expressing Cells in the Human Pancreas EP - 373 IS - 4 SP - 362 VL - 196 DO - 10.1159/000335469 ER -
@article{ author = "Vignjević, Sanja and Todorović, Vera and Damjanović, Svetozar and Budeč, Mirela and Mitrović, Olivera and Đikić, Dragoslava and Drndarević, Neda and Mićić, Mileva and Miskovic-Krivokapić, Jelena and Đuricić, Slavisa and Nikolić, Ivan", year = "2012", abstract = "The pancreas appears to be a major source of ghrelin during fetal development, but the ontogeny of ghrelin cells in the human pancreas and their developmental relationship with alpha- and beta-cells remain largely unknown. In the present study, we examined the dynamics of ghrelin cell growth, colocalization of ghrelin with major pancreatic hormones and defined the similarities and differences among developmental patterns of ghrelin-, glucagon- and insulin-expressing cells in the human pancreas. To this end, paraffin-embedded pancreatic tissue sections from human embryos and fetuses were assessed by immunohistochemistry. Ghrelin-positive cells were first detected in the pancreas of 11-week-old fetuses. With advancing gestational age, both ghrelin- and glucagon-expressing cells were increasingly observed at the periphery of the developing islets, whereas insulin-containing cells were typically found in the islet core. Double immunohistochemistry showed that ghrelin-expressing cells were clearly separate from insulin-, somatostatin- and pancreatic polypeptide-containing cells. In contrast, cells coexpressing ghrelin and glucagon were sporadically detected during both the early and late fetal periods. Furthermore, morphometric analysis revealed a similar trend in the volume density of ghrelin- and glucagon-positive cells, and a contrasting pattern in beta-cell density at specific time points during the development of the human pancreas. This study demonstrates that the developmental pattern of ghrelin cells, although clearly distinct, is quite similar to that of glucagon-expressing cells. The obtained findings indicate a close lineage relationship between these cell populations, a functional relationship between their secretory products and an auto/paracrine mode of ghrelin-glucagon interaction in pancreatic development. Copyright (C) 2012 S. Karger AG, Basel", publisher = "Karger, Basel", journal = "Cells Tissues Organs", title = "Similar Developmental Patterns of Ghrelin- and Glucagon-Expressing Cells in the Human Pancreas", pages = "373-362", number = "4", volume = "196", doi = "10.1159/000335469" }
Vignjević, S., Todorović, V., Damjanović, S., Budeč, M., Mitrović, O., Đikić, D., Drndarević, N., Mićić, M., Miskovic-Krivokapić, J., Đuricić, S.,& Nikolić, I.. (2012). Similar Developmental Patterns of Ghrelin- and Glucagon-Expressing Cells in the Human Pancreas. in Cells Tissues Organs Karger, Basel., 196(4), 362-373. https://doi.org/10.1159/000335469
Vignjević S, Todorović V, Damjanović S, Budeč M, Mitrović O, Đikić D, Drndarević N, Mićić M, Miskovic-Krivokapić J, Đuricić S, Nikolić I. Similar Developmental Patterns of Ghrelin- and Glucagon-Expressing Cells in the Human Pancreas. in Cells Tissues Organs. 2012;196(4):362-373. doi:10.1159/000335469 .
Vignjević, Sanja, Todorović, Vera, Damjanović, Svetozar, Budeč, Mirela, Mitrović, Olivera, Đikić, Dragoslava, Drndarević, Neda, Mićić, Mileva, Miskovic-Krivokapić, Jelena, Đuricić, Slavisa, Nikolić, Ivan, "Similar Developmental Patterns of Ghrelin- and Glucagon-Expressing Cells in the Human Pancreas" in Cells Tissues Organs, 196, no. 4 (2012):362-373, https://doi.org/10.1159/000335469 . .