Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors
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Krstić, AleksandraKocić, Jelena
Ilić, Vesna
Mojsilović, S.
Okić Đorđević, Ivana
Trivanović, Drenka
Santibanez, Juan F.
Jovčić, Gordana
Bugarski, Diana
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Interleukin-17 is Th17 cell cytokine implicated in regulation of hematopoiesis and inflammation. Besides promoting granulopoiesis, we have previously shown that IL-17 also affects erythropoiesis stimulating the development of early erythroid progenitors, BFU-E, but suppressing, at least partly via p38 MAPK, the growth of late stage erythroid progenitors, CFU-E. The aim of the present study was to investigate the involvement of other MAPKs, JNK and ERK1/2, as well as GATA transcription factors, in IL-17-mediated effects on murine bone marrow erythroid progenitors. Data obtained by use of specific MAPKs inhibitors indicated that MEK1/2-ERK1/2 MAPK signaling mediates IL-17-induced CFU-E inhibition, as well as that JNK and/or MEK1/2-ERK1/2 MAPKs activation underlies IL-17-induced stimulation of BFU-E growth. Furthermore, Western blot analyses demonstrated no effect on early hematopoiesis transcription factor, GATA-2, and enhanced expression level of erythroid-specific factor GATA-1 in muri...ne bone marrow cells after IL-17 stimulation, which in light of previous reports that GATA-1 overexpression inhibits erythroid differentiation, could be related to IL-17-mediated inhibition of CFU-E growth. Although, no contribution for p38, JNK and ERK MAPKs in IL-17-induced GATA-1 expression was shown, data obtained using specific inhibitors pointed to the role of JNK and MEK1/2-ERK1/2 in GATA-1 downregulation. Overall, obtained data gave an insight into the mechanisms by which IL-17 exerts its effects on erythropoiesis, implying the involvement of JNK and ERK MAPKs, as well as GATA-1, in IL-17-regulated growth of erythroid progentors.
Keywords:
IL-17 / erythropoiesis / MAPK / GATASource:
Journal of Biological Regulators & Homeostatic Agents, 2012, 26, 4, 641-652Publisher:
- Biolife Sas, Silva Marina (Te)
Funding / projects:
- Regenerative and modulatory potential of adult stem cells (RS-MESTD-Basic Research (BR or ON)-175062)
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Institut za medicinska istraživanjaTY - JOUR AU - Krstić, Aleksandra AU - Kocić, Jelena AU - Ilić, Vesna AU - Mojsilović, S. AU - Okić Đorđević, Ivana AU - Trivanović, Drenka AU - Santibanez, Juan F. AU - Jovčić, Gordana AU - Bugarski, Diana PY - 2012 UR - http://rimi.imi.bg.ac.rs/handle/123456789/403 AB - Interleukin-17 is Th17 cell cytokine implicated in regulation of hematopoiesis and inflammation. Besides promoting granulopoiesis, we have previously shown that IL-17 also affects erythropoiesis stimulating the development of early erythroid progenitors, BFU-E, but suppressing, at least partly via p38 MAPK, the growth of late stage erythroid progenitors, CFU-E. The aim of the present study was to investigate the involvement of other MAPKs, JNK and ERK1/2, as well as GATA transcription factors, in IL-17-mediated effects on murine bone marrow erythroid progenitors. Data obtained by use of specific MAPKs inhibitors indicated that MEK1/2-ERK1/2 MAPK signaling mediates IL-17-induced CFU-E inhibition, as well as that JNK and/or MEK1/2-ERK1/2 MAPKs activation underlies IL-17-induced stimulation of BFU-E growth. Furthermore, Western blot analyses demonstrated no effect on early hematopoiesis transcription factor, GATA-2, and enhanced expression level of erythroid-specific factor GATA-1 in murine bone marrow cells after IL-17 stimulation, which in light of previous reports that GATA-1 overexpression inhibits erythroid differentiation, could be related to IL-17-mediated inhibition of CFU-E growth. Although, no contribution for p38, JNK and ERK MAPKs in IL-17-induced GATA-1 expression was shown, data obtained using specific inhibitors pointed to the role of JNK and MEK1/2-ERK1/2 in GATA-1 downregulation. Overall, obtained data gave an insight into the mechanisms by which IL-17 exerts its effects on erythropoiesis, implying the involvement of JNK and ERK MAPKs, as well as GATA-1, in IL-17-regulated growth of erythroid progentors. PB - Biolife Sas, Silva Marina (Te) T2 - Journal of Biological Regulators & Homeostatic Agents T1 - Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors EP - 652 IS - 4 SP - 641 VL - 26 UR - https://hdl.handle.net/21.15107/rcub_rimi_403 ER -
@article{ author = "Krstić, Aleksandra and Kocić, Jelena and Ilić, Vesna and Mojsilović, S. and Okić Đorđević, Ivana and Trivanović, Drenka and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana", year = "2012", abstract = "Interleukin-17 is Th17 cell cytokine implicated in regulation of hematopoiesis and inflammation. Besides promoting granulopoiesis, we have previously shown that IL-17 also affects erythropoiesis stimulating the development of early erythroid progenitors, BFU-E, but suppressing, at least partly via p38 MAPK, the growth of late stage erythroid progenitors, CFU-E. The aim of the present study was to investigate the involvement of other MAPKs, JNK and ERK1/2, as well as GATA transcription factors, in IL-17-mediated effects on murine bone marrow erythroid progenitors. Data obtained by use of specific MAPKs inhibitors indicated that MEK1/2-ERK1/2 MAPK signaling mediates IL-17-induced CFU-E inhibition, as well as that JNK and/or MEK1/2-ERK1/2 MAPKs activation underlies IL-17-induced stimulation of BFU-E growth. Furthermore, Western blot analyses demonstrated no effect on early hematopoiesis transcription factor, GATA-2, and enhanced expression level of erythroid-specific factor GATA-1 in murine bone marrow cells after IL-17 stimulation, which in light of previous reports that GATA-1 overexpression inhibits erythroid differentiation, could be related to IL-17-mediated inhibition of CFU-E growth. Although, no contribution for p38, JNK and ERK MAPKs in IL-17-induced GATA-1 expression was shown, data obtained using specific inhibitors pointed to the role of JNK and MEK1/2-ERK1/2 in GATA-1 downregulation. Overall, obtained data gave an insight into the mechanisms by which IL-17 exerts its effects on erythropoiesis, implying the involvement of JNK and ERK MAPKs, as well as GATA-1, in IL-17-regulated growth of erythroid progentors.", publisher = "Biolife Sas, Silva Marina (Te)", journal = "Journal of Biological Regulators & Homeostatic Agents", title = "Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors", pages = "652-641", number = "4", volume = "26", url = "https://hdl.handle.net/21.15107/rcub_rimi_403" }
Krstić, A., Kocić, J., Ilić, V., Mojsilović, S., Okić Đorđević, I., Trivanović, D., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2012). Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors. in Journal of Biological Regulators & Homeostatic Agents Biolife Sas, Silva Marina (Te)., 26(4), 641-652. https://hdl.handle.net/21.15107/rcub_rimi_403
Krstić A, Kocić J, Ilić V, Mojsilović S, Okić Đorđević I, Trivanović D, Santibanez JF, Jovčić G, Bugarski D. Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors. in Journal of Biological Regulators & Homeostatic Agents. 2012;26(4):641-652. https://hdl.handle.net/21.15107/rcub_rimi_403 .
Krstić, Aleksandra, Kocić, Jelena, Ilić, Vesna, Mojsilović, S., Okić Đorđević, Ivana, Trivanović, Drenka, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors" in Journal of Biological Regulators & Homeostatic Agents, 26, no. 4 (2012):641-652, https://hdl.handle.net/21.15107/rcub_rimi_403 .