17 beta-estradiol modulates mitochondrial ca(2+) flux in rat caudate nucleus and brain stem
Само за регистроване кориснике
2012
Аутори
Petrović, SnježanaMilošević, Maja
Drakulić, Dunja
Grković, Ivana
Stanojlovio, M.
Mitrović, N.
Horvat, Anica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to examine the rapid non-genomic effect of 17 beta-estradiol (E2) on Ca2+ transport in mitochondria isolated from the nerve terminals (synaptosomes) of caudate nuclei (NC) and brain stems (BS) of ovariectomised female rats. In physiological conditions no effect of E2 on Ca2+ influx into synaptosomal mitochondria through ruthenium red (RR)-sensitive uniporter was observed. However, in the presence of uncoupling agent carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone (FCCP) (1 mu mol/l), pre-treatment with 0.5 nmol/l E2 protected mitochondrial membrane potential and consequently increased Ca2+ influx (2.3-fold in NC and 3.1-fold in BS). At the same time, 0.5 nmol/l E2 by increasing the affinity of mitochondrial Na+/Ca2+ exchanger for Na+ inhibited mitochondrial Ca2+ efflux in NC and BS by about 40%. Also, the specific binding of physiological E2 concentrations (0.1-10 nmol/l) to isolated synaptosomal mitochondria was detected. Using membrane impermeable E2 bou...nd to bovine serum albumin and selective inhibitor of mitochondrial Na+/Ca2+ exchanger, we obtained that E2's action on mitochondrial Ca2+ efflux at least partially is due to the direct effects on the mitochondrial membrane and/or Na+/Ca2+ exchanger located in inner mitochondrial membrane. Our results implicate E2 as a modulator of Ca2+ concentration in mitochondrial matrix, and ultimately in the cytosol. Given the vital role of Ca2+ in regulation of total nerve cells activity, especially energy metabolism, neurotransmission and directing the cells toward survival or cell death, the effects on mitochondrial Ca2+ transport could be one of the important modes of E2 neuromodulatory action independent of the genome.
Кључне речи:
synaptosomal mitochondria / Ca2+ transport / estradiol / rat brain / caudate nucleus / brain stemИзвор:
Neuroscience, 2012, 220, 32-40Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
Финансирање / пројекти:
DOI: 10.1016/j.neuroscience.2012.06.040
ISSN: 0306-4522
PubMed: 22735576
WoS: 000307802100005
Scopus: 2-s2.0-84864780476
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Petrović, Snježana AU - Milošević, Maja AU - Drakulić, Dunja AU - Grković, Ivana AU - Stanojlovio, M. AU - Mitrović, N. AU - Horvat, Anica PY - 2012 UR - http://rimi.imi.bg.ac.rs/handle/123456789/400 AB - The aim of this study was to examine the rapid non-genomic effect of 17 beta-estradiol (E2) on Ca2+ transport in mitochondria isolated from the nerve terminals (synaptosomes) of caudate nuclei (NC) and brain stems (BS) of ovariectomised female rats. In physiological conditions no effect of E2 on Ca2+ influx into synaptosomal mitochondria through ruthenium red (RR)-sensitive uniporter was observed. However, in the presence of uncoupling agent carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone (FCCP) (1 mu mol/l), pre-treatment with 0.5 nmol/l E2 protected mitochondrial membrane potential and consequently increased Ca2+ influx (2.3-fold in NC and 3.1-fold in BS). At the same time, 0.5 nmol/l E2 by increasing the affinity of mitochondrial Na+/Ca2+ exchanger for Na+ inhibited mitochondrial Ca2+ efflux in NC and BS by about 40%. Also, the specific binding of physiological E2 concentrations (0.1-10 nmol/l) to isolated synaptosomal mitochondria was detected. Using membrane impermeable E2 bound to bovine serum albumin and selective inhibitor of mitochondrial Na+/Ca2+ exchanger, we obtained that E2's action on mitochondrial Ca2+ efflux at least partially is due to the direct effects on the mitochondrial membrane and/or Na+/Ca2+ exchanger located in inner mitochondrial membrane. Our results implicate E2 as a modulator of Ca2+ concentration in mitochondrial matrix, and ultimately in the cytosol. Given the vital role of Ca2+ in regulation of total nerve cells activity, especially energy metabolism, neurotransmission and directing the cells toward survival or cell death, the effects on mitochondrial Ca2+ transport could be one of the important modes of E2 neuromodulatory action independent of the genome. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Neuroscience T1 - 17 beta-estradiol modulates mitochondrial ca(2+) flux in rat caudate nucleus and brain stem EP - 40 SP - 32 VL - 220 DO - 10.1016/j.neuroscience.2012.06.040 ER -
@article{ author = "Petrović, Snježana and Milošević, Maja and Drakulić, Dunja and Grković, Ivana and Stanojlovio, M. and Mitrović, N. and Horvat, Anica", year = "2012", abstract = "The aim of this study was to examine the rapid non-genomic effect of 17 beta-estradiol (E2) on Ca2+ transport in mitochondria isolated from the nerve terminals (synaptosomes) of caudate nuclei (NC) and brain stems (BS) of ovariectomised female rats. In physiological conditions no effect of E2 on Ca2+ influx into synaptosomal mitochondria through ruthenium red (RR)-sensitive uniporter was observed. However, in the presence of uncoupling agent carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone (FCCP) (1 mu mol/l), pre-treatment with 0.5 nmol/l E2 protected mitochondrial membrane potential and consequently increased Ca2+ influx (2.3-fold in NC and 3.1-fold in BS). At the same time, 0.5 nmol/l E2 by increasing the affinity of mitochondrial Na+/Ca2+ exchanger for Na+ inhibited mitochondrial Ca2+ efflux in NC and BS by about 40%. Also, the specific binding of physiological E2 concentrations (0.1-10 nmol/l) to isolated synaptosomal mitochondria was detected. Using membrane impermeable E2 bound to bovine serum albumin and selective inhibitor of mitochondrial Na+/Ca2+ exchanger, we obtained that E2's action on mitochondrial Ca2+ efflux at least partially is due to the direct effects on the mitochondrial membrane and/or Na+/Ca2+ exchanger located in inner mitochondrial membrane. Our results implicate E2 as a modulator of Ca2+ concentration in mitochondrial matrix, and ultimately in the cytosol. Given the vital role of Ca2+ in regulation of total nerve cells activity, especially energy metabolism, neurotransmission and directing the cells toward survival or cell death, the effects on mitochondrial Ca2+ transport could be one of the important modes of E2 neuromodulatory action independent of the genome.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Neuroscience", title = "17 beta-estradiol modulates mitochondrial ca(2+) flux in rat caudate nucleus and brain stem", pages = "40-32", volume = "220", doi = "10.1016/j.neuroscience.2012.06.040" }
Petrović, S., Milošević, M., Drakulić, D., Grković, I., Stanojlovio, M., Mitrović, N.,& Horvat, A.. (2012). 17 beta-estradiol modulates mitochondrial ca(2+) flux in rat caudate nucleus and brain stem. in Neuroscience Pergamon-Elsevier Science Ltd, Oxford., 220, 32-40. https://doi.org/10.1016/j.neuroscience.2012.06.040
Petrović S, Milošević M, Drakulić D, Grković I, Stanojlovio M, Mitrović N, Horvat A. 17 beta-estradiol modulates mitochondrial ca(2+) flux in rat caudate nucleus and brain stem. in Neuroscience. 2012;220:32-40. doi:10.1016/j.neuroscience.2012.06.040 .
Petrović, Snježana, Milošević, Maja, Drakulić, Dunja, Grković, Ivana, Stanojlovio, M., Mitrović, N., Horvat, Anica, "17 beta-estradiol modulates mitochondrial ca(2+) flux in rat caudate nucleus and brain stem" in Neuroscience, 220 (2012):32-40, https://doi.org/10.1016/j.neuroscience.2012.06.040 . .