Syphacia obvelata modifies mitogen-activated protein kinases and nitric oxide synthases expression in murine bone marrow cells
Само за регистроване кориснике
2010
Аутори
Ilić, VesnaKrstić, Aleksandra
Katić-Radivojević, Sofija
Jovčić, Gordana
Milenković, Pavle B.
Bugarski, Diana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Syphacia obvelata is a rodent nematode parasite with high prevalence in laboratory mice. In our previous work we have demonstrated that this gut-dwelling helminth induces significant hematopoietic changes, characterized by increased myelopoiesis and erythropoiesis in infected animals, and accompanied with altered reactivity of bone marrow hematopoietic progenitors to interleukin (IL)-17. In this study we extended these investigations by demonstrating that naturally acquired S. obvelata infection induces significant alterations in murine bone marrow cells manifested at the molecular level. Namely, S. obvelata infection induced Sustained phosphorylation of the members of three major groups of distinctly regulated mitogen-activated protein kinases (MAPKs), the p38, the c-Jun amino-terminal kinase (JNK) and the extracellular signal-regulated kinase (ERK), as well as enhanced expression of mRNA for the inducible nitric oxide synthase (iNOS) in the bone marrow cells of infected animals. Furt...hermore, the infection interfered with the IL-17-mediated effects in bone marrow cells, since in normal mice IL-17 significantly enhanced phosphorylation of p38 MAPK and upregulated the expression of NOS and the constitutive, endothelial (e)NOS mRNA, while in S. obvelata-infected animals IL-17 did not influence the MAPKs activation, but markedly down-regulated the expression of both NOS isoforms. The data obtained demonstrating that S. obvelata is able to manipulate signal transduction pathways in the hosts' bone marrow cells, pointed to the multiple layers of immuno modulatory ability of this pinworm parasite and highlighted the importance of working under pinworm-free conditions when using experimental murine models for immunohematopoietic investigations.
Кључне речи:
IL-17 / Bone marrow / Signal transduction / MAPK / NOS / PinwormИзвор:
Parasitology International, 2010, 59, 1, 82-88Издавач:
- Elsevier Ireland Ltd, Clare
Финансирање / пројекти:
- Ћелијски и молекуларни механизми регилације хематопоезе (RS-MESTD-MPN2006-2010-145048)
DOI: 10.1016/j.parint.2009.10.011
ISSN: 1383-5769
PubMed: 19903537
WoS: 000275777600014
Scopus: 2-s2.0-76349086449
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Ilić, Vesna AU - Krstić, Aleksandra AU - Katić-Radivojević, Sofija AU - Jovčić, Gordana AU - Milenković, Pavle B. AU - Bugarski, Diana PY - 2010 UR - http://rimi.imi.bg.ac.rs/handle/123456789/321 AB - Syphacia obvelata is a rodent nematode parasite with high prevalence in laboratory mice. In our previous work we have demonstrated that this gut-dwelling helminth induces significant hematopoietic changes, characterized by increased myelopoiesis and erythropoiesis in infected animals, and accompanied with altered reactivity of bone marrow hematopoietic progenitors to interleukin (IL)-17. In this study we extended these investigations by demonstrating that naturally acquired S. obvelata infection induces significant alterations in murine bone marrow cells manifested at the molecular level. Namely, S. obvelata infection induced Sustained phosphorylation of the members of three major groups of distinctly regulated mitogen-activated protein kinases (MAPKs), the p38, the c-Jun amino-terminal kinase (JNK) and the extracellular signal-regulated kinase (ERK), as well as enhanced expression of mRNA for the inducible nitric oxide synthase (iNOS) in the bone marrow cells of infected animals. Furthermore, the infection interfered with the IL-17-mediated effects in bone marrow cells, since in normal mice IL-17 significantly enhanced phosphorylation of p38 MAPK and upregulated the expression of NOS and the constitutive, endothelial (e)NOS mRNA, while in S. obvelata-infected animals IL-17 did not influence the MAPKs activation, but markedly down-regulated the expression of both NOS isoforms. The data obtained demonstrating that S. obvelata is able to manipulate signal transduction pathways in the hosts' bone marrow cells, pointed to the multiple layers of immuno modulatory ability of this pinworm parasite and highlighted the importance of working under pinworm-free conditions when using experimental murine models for immunohematopoietic investigations. PB - Elsevier Ireland Ltd, Clare T2 - Parasitology International T1 - Syphacia obvelata modifies mitogen-activated protein kinases and nitric oxide synthases expression in murine bone marrow cells EP - 88 IS - 1 SP - 82 VL - 59 DO - 10.1016/j.parint.2009.10.011 ER -
@article{ author = "Ilić, Vesna and Krstić, Aleksandra and Katić-Radivojević, Sofija and Jovčić, Gordana and Milenković, Pavle B. and Bugarski, Diana", year = "2010", abstract = "Syphacia obvelata is a rodent nematode parasite with high prevalence in laboratory mice. In our previous work we have demonstrated that this gut-dwelling helminth induces significant hematopoietic changes, characterized by increased myelopoiesis and erythropoiesis in infected animals, and accompanied with altered reactivity of bone marrow hematopoietic progenitors to interleukin (IL)-17. In this study we extended these investigations by demonstrating that naturally acquired S. obvelata infection induces significant alterations in murine bone marrow cells manifested at the molecular level. Namely, S. obvelata infection induced Sustained phosphorylation of the members of three major groups of distinctly regulated mitogen-activated protein kinases (MAPKs), the p38, the c-Jun amino-terminal kinase (JNK) and the extracellular signal-regulated kinase (ERK), as well as enhanced expression of mRNA for the inducible nitric oxide synthase (iNOS) in the bone marrow cells of infected animals. Furthermore, the infection interfered with the IL-17-mediated effects in bone marrow cells, since in normal mice IL-17 significantly enhanced phosphorylation of p38 MAPK and upregulated the expression of NOS and the constitutive, endothelial (e)NOS mRNA, while in S. obvelata-infected animals IL-17 did not influence the MAPKs activation, but markedly down-regulated the expression of both NOS isoforms. The data obtained demonstrating that S. obvelata is able to manipulate signal transduction pathways in the hosts' bone marrow cells, pointed to the multiple layers of immuno modulatory ability of this pinworm parasite and highlighted the importance of working under pinworm-free conditions when using experimental murine models for immunohematopoietic investigations.", publisher = "Elsevier Ireland Ltd, Clare", journal = "Parasitology International", title = "Syphacia obvelata modifies mitogen-activated protein kinases and nitric oxide synthases expression in murine bone marrow cells", pages = "88-82", number = "1", volume = "59", doi = "10.1016/j.parint.2009.10.011" }
Ilić, V., Krstić, A., Katić-Radivojević, S., Jovčić, G., Milenković, P. B.,& Bugarski, D.. (2010). Syphacia obvelata modifies mitogen-activated protein kinases and nitric oxide synthases expression in murine bone marrow cells. in Parasitology International Elsevier Ireland Ltd, Clare., 59(1), 82-88. https://doi.org/10.1016/j.parint.2009.10.011
Ilić V, Krstić A, Katić-Radivojević S, Jovčić G, Milenković PB, Bugarski D. Syphacia obvelata modifies mitogen-activated protein kinases and nitric oxide synthases expression in murine bone marrow cells. in Parasitology International. 2010;59(1):82-88. doi:10.1016/j.parint.2009.10.011 .
Ilić, Vesna, Krstić, Aleksandra, Katić-Radivojević, Sofija, Jovčić, Gordana, Milenković, Pavle B., Bugarski, Diana, "Syphacia obvelata modifies mitogen-activated protein kinases and nitric oxide synthases expression in murine bone marrow cells" in Parasitology International, 59, no. 1 (2010):82-88, https://doi.org/10.1016/j.parint.2009.10.011 . .