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Mechanisms involved in the binding of thymocytes to rat thymic dendritic cells
dc.creator | Colić, M | |
dc.creator | Ilić, Vesna | |
dc.creator | Pavlović, MD | |
dc.creator | Tamatani, T. | |
dc.creator | Miyasaka, M | |
dc.date.accessioned | 2021-04-20T12:03:15Z | |
dc.date.available | 2021-04-20T12:03:15Z | |
dc.date.issued | 1996 | |
dc.identifier.issn | 1044-6672 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/28 | |
dc.description.abstract | The effects of monoclonal antibodies (mAbs) to cell-surface molecules, divalent cations, and various cell-signaling and metabolic inhibitors on the binding of thymocytes to rat thymic dendritic cells (TDC) were studied using a rosette assay. It was found that TDC/thymocyte adhesion was stronger and faster at 37 degrees C than at 4 degrees C. Flow cytometric analysis demonstrated that bound thymocytes were predominantly CD4(+)CD8(+) and CD4(+)CD8(-), but in comparison to the phenotype of whole thymocytes, they were enriched in the mature TCR alpha beta(hi) subset. The binding of thymocytes to TDC at 37 degrees C was almost completely dependent on Ca2+ and Mg2+ and partly on an intact cytoskeleton and calmodulin-dependent protein kinase. The adhesion was independent of new protein synthesis and the activities of protein kinases A and C, tyrosine kinases, as well as phosphotyrosine protein phosphatases. The TDC/thymocyte adhesion at 37 degrees C was partly blocked by anti-LFA-l (WT.1), anti-CD18 (WT.3), and anti-ICAM-1 (1A29) mAb. MAbs to class II MHC (OX-3 and OX-6), CD4 (W3/25), CD8 (OX-5), and alpha beta TCR (R73) stimulated the adhesion via an LFA-1-dependent pathway, whereas an anti-CD45 mAb (G3C5) stimulated the rosette formation independently of LFA-1. MAbs to CD2 (OX-34), CD11b (ED7), CD11b/c (OX-42), and class I MHC (OX-18) were without significant effects on the adhesion process. | en |
dc.publisher | Harwood Acad Publ Gmbh, Taylor & Francis Group, Philadelphia | |
dc.rights | openAccess | |
dc.source | Developmental Immunology | |
dc.subject | thymic dendritic cells | en |
dc.subject | thymocytes | en |
dc.subject | adhesion molecules | en |
dc.subject | rosettes | en |
dc.subject | signaling | en |
dc.title | Mechanisms involved in the binding of thymocytes to rat thymic dendritic cells | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 51 | |
dc.citation.issue | 1 | |
dc.citation.other | 5(1): 37-51 | |
dc.citation.spage | 37 | |
dc.citation.volume | 5 | |
dc.identifier.doi | 10.1155/1996/18312 | |
dc.identifier.fulltext | http://rimi.imi.bg.ac.rs/bitstream/id/211/25.pdf | |
dc.identifier.pmid | 8828010 | |
dc.identifier.scopus | 2-s2.0-0029813642 | |
dc.identifier.wos | A1996UY57300004 | |
dc.type.version | publishedVersion |