Quantification of oxidative stress markers in the blood sera following subacute administration of different oximes in rats
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2024
Authors
Jaćević, VesnaGrujić-Milanović, Jelica
Milovanović, Zoran
Nežić, Lana
Amidžić, Ljiljana
Vojinović, Nataša
Marković, Bojan
Dobričić, Vladimir
Milosavljević, Petar
Nepovimova, Eugenie
Kuča, Kamil
Article (Published version)
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Oxidative stress status, as a disruption of redox homeostasis, in the blood sera of Wistar rats caused by repeated application of selected acetylcholinesterase reactivators - asoxime, obidoxime, K027, K048, K074, and K075 were evaluated. Throughout this study, each oxime in a dose of 0.1 of LD50/kg im was given 2x/week for 4 weeks. Then, seven days after the last oximes' application, markers of lipid peroxidation (malondialdehyde, MDA), and protein oxidation (advanced oxidation protein products, AOPP), as well as the activity of antioxidant enzymes (catalase, CAT, superoxide dismutase, SOD, reduced glutathione, GSH, and oxidized glutathione, GSSG), were determined. Oxidative stress parameters, MDA and AOPP were significantly highest in the K048-, K074- and K075-treated groups (p < 0.001). The activity of CAT was significantly elevated in the obidoxime-treated group (p < 0.05), while treatment with K027, K048, and K074 induced high elevation in SOD levels (p < 0.01, p < 0.001). Interest...ingly, the activity of GSH in each oxime-treated group was significantly elevated. Unlike, treatment with obidoxime caused elevation in GSSG levels (p < 0.01). As a continuation of our previously published data, these results assure that applied oximes following subacute treatment ameliorated the oxidative status and further adverse systemic toxic effects in rats.
Keywords:
Oximes / Subacute toxicity / Blood sera / Oxidative stress / RatsSource:
Chemico-Biological Interactions, 2024, 399, 111138-Publisher:
- Elsevier
Funding / projects:
- Medical Faculty of the Military Medical Academy, University of Defence in Belgrade, Serbia (MFVMA01/ 23–25)
- the Excellence project PrF UHK 2216/2023–2024
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200015 (University of Belgrade, Institute for Medical Research) (RS-MESTD-inst-2020-200015)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200161 (University of Belgrade, Faculty of Pharmacy) (RS-MESTD-inst-2020-200161)
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Institut za medicinska istraživanjaTY - JOUR AU - Jaćević, Vesna AU - Grujić-Milanović, Jelica AU - Milovanović, Zoran AU - Nežić, Lana AU - Amidžić, Ljiljana AU - Vojinović, Nataša AU - Marković, Bojan AU - Dobričić, Vladimir AU - Milosavljević, Petar AU - Nepovimova, Eugenie AU - Kuča, Kamil PY - 2024 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1507 AB - Oxidative stress status, as a disruption of redox homeostasis, in the blood sera of Wistar rats caused by repeated application of selected acetylcholinesterase reactivators - asoxime, obidoxime, K027, K048, K074, and K075 were evaluated. Throughout this study, each oxime in a dose of 0.1 of LD50/kg im was given 2x/week for 4 weeks. Then, seven days after the last oximes' application, markers of lipid peroxidation (malondialdehyde, MDA), and protein oxidation (advanced oxidation protein products, AOPP), as well as the activity of antioxidant enzymes (catalase, CAT, superoxide dismutase, SOD, reduced glutathione, GSH, and oxidized glutathione, GSSG), were determined. Oxidative stress parameters, MDA and AOPP were significantly highest in the K048-, K074- and K075-treated groups (p < 0.001). The activity of CAT was significantly elevated in the obidoxime-treated group (p < 0.05), while treatment with K027, K048, and K074 induced high elevation in SOD levels (p < 0.01, p < 0.001). Interestingly, the activity of GSH in each oxime-treated group was significantly elevated. Unlike, treatment with obidoxime caused elevation in GSSG levels (p < 0.01). As a continuation of our previously published data, these results assure that applied oximes following subacute treatment ameliorated the oxidative status and further adverse systemic toxic effects in rats. PB - Elsevier T2 - Chemico-Biological Interactions T1 - Quantification of oxidative stress markers in the blood sera following subacute administration of different oximes in rats SP - 111138 VL - 399 DO - 10.1016/j.cbi.2024.111138 ER -
@article{ author = "Jaćević, Vesna and Grujić-Milanović, Jelica and Milovanović, Zoran and Nežić, Lana and Amidžić, Ljiljana and Vojinović, Nataša and Marković, Bojan and Dobričić, Vladimir and Milosavljević, Petar and Nepovimova, Eugenie and Kuča, Kamil", year = "2024", abstract = "Oxidative stress status, as a disruption of redox homeostasis, in the blood sera of Wistar rats caused by repeated application of selected acetylcholinesterase reactivators - asoxime, obidoxime, K027, K048, K074, and K075 were evaluated. Throughout this study, each oxime in a dose of 0.1 of LD50/kg im was given 2x/week for 4 weeks. Then, seven days after the last oximes' application, markers of lipid peroxidation (malondialdehyde, MDA), and protein oxidation (advanced oxidation protein products, AOPP), as well as the activity of antioxidant enzymes (catalase, CAT, superoxide dismutase, SOD, reduced glutathione, GSH, and oxidized glutathione, GSSG), were determined. Oxidative stress parameters, MDA and AOPP were significantly highest in the K048-, K074- and K075-treated groups (p < 0.001). The activity of CAT was significantly elevated in the obidoxime-treated group (p < 0.05), while treatment with K027, K048, and K074 induced high elevation in SOD levels (p < 0.01, p < 0.001). Interestingly, the activity of GSH in each oxime-treated group was significantly elevated. Unlike, treatment with obidoxime caused elevation in GSSG levels (p < 0.01). As a continuation of our previously published data, these results assure that applied oximes following subacute treatment ameliorated the oxidative status and further adverse systemic toxic effects in rats.", publisher = "Elsevier", journal = "Chemico-Biological Interactions", title = "Quantification of oxidative stress markers in the blood sera following subacute administration of different oximes in rats", pages = "111138", volume = "399", doi = "10.1016/j.cbi.2024.111138" }
Jaćević, V., Grujić-Milanović, J., Milovanović, Z., Nežić, L., Amidžić, L., Vojinović, N., Marković, B., Dobričić, V., Milosavljević, P., Nepovimova, E.,& Kuča, K.. (2024). Quantification of oxidative stress markers in the blood sera following subacute administration of different oximes in rats. in Chemico-Biological Interactions Elsevier., 399, 111138. https://doi.org/10.1016/j.cbi.2024.111138
Jaćević V, Grujić-Milanović J, Milovanović Z, Nežić L, Amidžić L, Vojinović N, Marković B, Dobričić V, Milosavljević P, Nepovimova E, Kuča K. Quantification of oxidative stress markers in the blood sera following subacute administration of different oximes in rats. in Chemico-Biological Interactions. 2024;399:111138. doi:10.1016/j.cbi.2024.111138 .
Jaćević, Vesna, Grujić-Milanović, Jelica, Milovanović, Zoran, Nežić, Lana, Amidžić, Ljiljana, Vojinović, Nataša, Marković, Bojan, Dobričić, Vladimir, Milosavljević, Petar, Nepovimova, Eugenie, Kuča, Kamil, "Quantification of oxidative stress markers in the blood sera following subacute administration of different oximes in rats" in Chemico-Biological Interactions, 399 (2024):111138, https://doi.org/10.1016/j.cbi.2024.111138 . .