Liver phospholipid fatty acid composition in response to chronic high-fat diets
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2024
Authors
Popović, TamaraNenadović, Aleksandra
Stanković, Anica
Debeljak Martačić, Jasmina
Ranković, Slavica
Kovačević, Sanjin
Nešović Ostojić, Jelena
Ilić, Anđelija
Milašin, Jelena
De Luka, Silvio
Trbovich, Alexander M.
Article (Published version)
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Show full item recordAbstract
Liver phospholipid fatty acid composition depends on the dietary lipid intake and the efficiency of hepatic enzymatic activity. Our study aimed to simultaneously investigate the liver phospholipid fatty acid composition in response to chronic linseed, palm, or sunflower oil diets. We used adult female C57/BL6 mice and randomly divided them into control and three groups treated with 25 % dietary oils. Prior to treatment, we analyzed the fatty acid profiles in dietary oils and hepatocytes and, after 100 days, the fatty acid composition in the liver using gas-liquid chromatography. Linseed oil treatment elevated alpha-linolenic, eicosapentaenoic, and docosapentaenoic acids and reduced arachidonic and docosatetraenoic acids, consequently lowering the n-6/n-3 ratio. Palm oil treatment increased linoleic acid and decreased docosahexaenoic acid, contributing to an elevated n-6/n-3 ratio. Sunflower oil treatment elevated total monounsaturated fatty acids by increasing palmitoleic, oleic, and v...accenic acids. The estimated activity of Δ9 desaturase was significantly elevated in the sunflower oil group, while Δ5 desaturase was the highest, and Δ6 desaturase was the lowest after the linseed oil diet. Our findings demonstrate that chronic consumption of linseed, palm, or sunflower oil alters the distribution of liver phospholipid fatty acids differently. Sunflower oil diet elevated total monounsaturated fatty acids, proposing potential benefits for liver tissue health. Considering these outcomes, a substantial recommendation emerges to elevate linseed oil intake, recognized as the principal ALA source, thereby aiding in reducing the n-6/n-3 ratio. Moreover, modifying dietary habits to incorporate specific vegetable oils in daily consumption could substantially enhance overall health.
Keywords:
Chronic high-fat diet / Liver / Linseed oil / Palm oil / Phospholipid fatty acids / Sunflower oilSource:
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2024, 1869, 7, 159526-Publisher:
- Elsevier
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200110 (University of Belgrade, Faculty of Medicine) (RS-MESTD-inst-2020-200110)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200015 (University of Belgrade, Institute for Medical Research) (RS-MESTD-inst-2020-200015)
DOI: 10.1016/j.bbalip.2024.159526
ISSN: 1388-1981
PubMed: 38871115
WoS: 001257875000001
Scopus: 2-s2.0-85196015576
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Institut za medicinska istraživanjaTY - JOUR AU - Popović, Tamara AU - Nenadović, Aleksandra AU - Stanković, Anica AU - Debeljak Martačić, Jasmina AU - Ranković, Slavica AU - Kovačević, Sanjin AU - Nešović Ostojić, Jelena AU - Ilić, Anđelija AU - Milašin, Jelena AU - De Luka, Silvio AU - Trbovich, Alexander M. PY - 2024 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1502 AB - Liver phospholipid fatty acid composition depends on the dietary lipid intake and the efficiency of hepatic enzymatic activity. Our study aimed to simultaneously investigate the liver phospholipid fatty acid composition in response to chronic linseed, palm, or sunflower oil diets. We used adult female C57/BL6 mice and randomly divided them into control and three groups treated with 25 % dietary oils. Prior to treatment, we analyzed the fatty acid profiles in dietary oils and hepatocytes and, after 100 days, the fatty acid composition in the liver using gas-liquid chromatography. Linseed oil treatment elevated alpha-linolenic, eicosapentaenoic, and docosapentaenoic acids and reduced arachidonic and docosatetraenoic acids, consequently lowering the n-6/n-3 ratio. Palm oil treatment increased linoleic acid and decreased docosahexaenoic acid, contributing to an elevated n-6/n-3 ratio. Sunflower oil treatment elevated total monounsaturated fatty acids by increasing palmitoleic, oleic, and vaccenic acids. The estimated activity of Δ9 desaturase was significantly elevated in the sunflower oil group, while Δ5 desaturase was the highest, and Δ6 desaturase was the lowest after the linseed oil diet. Our findings demonstrate that chronic consumption of linseed, palm, or sunflower oil alters the distribution of liver phospholipid fatty acids differently. Sunflower oil diet elevated total monounsaturated fatty acids, proposing potential benefits for liver tissue health. Considering these outcomes, a substantial recommendation emerges to elevate linseed oil intake, recognized as the principal ALA source, thereby aiding in reducing the n-6/n-3 ratio. Moreover, modifying dietary habits to incorporate specific vegetable oils in daily consumption could substantially enhance overall health. PB - Elsevier T2 - Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids T1 - Liver phospholipid fatty acid composition in response to chronic high-fat diets IS - 7 SP - 159526 VL - 1869 DO - 10.1016/j.bbalip.2024.159526 ER -
@article{ author = "Popović, Tamara and Nenadović, Aleksandra and Stanković, Anica and Debeljak Martačić, Jasmina and Ranković, Slavica and Kovačević, Sanjin and Nešović Ostojić, Jelena and Ilić, Anđelija and Milašin, Jelena and De Luka, Silvio and Trbovich, Alexander M.", year = "2024", abstract = "Liver phospholipid fatty acid composition depends on the dietary lipid intake and the efficiency of hepatic enzymatic activity. Our study aimed to simultaneously investigate the liver phospholipid fatty acid composition in response to chronic linseed, palm, or sunflower oil diets. We used adult female C57/BL6 mice and randomly divided them into control and three groups treated with 25 % dietary oils. Prior to treatment, we analyzed the fatty acid profiles in dietary oils and hepatocytes and, after 100 days, the fatty acid composition in the liver using gas-liquid chromatography. Linseed oil treatment elevated alpha-linolenic, eicosapentaenoic, and docosapentaenoic acids and reduced arachidonic and docosatetraenoic acids, consequently lowering the n-6/n-3 ratio. Palm oil treatment increased linoleic acid and decreased docosahexaenoic acid, contributing to an elevated n-6/n-3 ratio. Sunflower oil treatment elevated total monounsaturated fatty acids by increasing palmitoleic, oleic, and vaccenic acids. The estimated activity of Δ9 desaturase was significantly elevated in the sunflower oil group, while Δ5 desaturase was the highest, and Δ6 desaturase was the lowest after the linseed oil diet. Our findings demonstrate that chronic consumption of linseed, palm, or sunflower oil alters the distribution of liver phospholipid fatty acids differently. Sunflower oil diet elevated total monounsaturated fatty acids, proposing potential benefits for liver tissue health. Considering these outcomes, a substantial recommendation emerges to elevate linseed oil intake, recognized as the principal ALA source, thereby aiding in reducing the n-6/n-3 ratio. Moreover, modifying dietary habits to incorporate specific vegetable oils in daily consumption could substantially enhance overall health.", publisher = "Elsevier", journal = "Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids", title = "Liver phospholipid fatty acid composition in response to chronic high-fat diets", number = "7", pages = "159526", volume = "1869", doi = "10.1016/j.bbalip.2024.159526" }
Popović, T., Nenadović, A., Stanković, A., Debeljak Martačić, J., Ranković, S., Kovačević, S., Nešović Ostojić, J., Ilić, A., Milašin, J., De Luka, S.,& Trbovich, A. M.. (2024). Liver phospholipid fatty acid composition in response to chronic high-fat diets. in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Elsevier., 1869(7), 159526. https://doi.org/10.1016/j.bbalip.2024.159526
Popović T, Nenadović A, Stanković A, Debeljak Martačić J, Ranković S, Kovačević S, Nešović Ostojić J, Ilić A, Milašin J, De Luka S, Trbovich AM. Liver phospholipid fatty acid composition in response to chronic high-fat diets. in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 2024;1869(7):159526. doi:10.1016/j.bbalip.2024.159526 .
Popović, Tamara, Nenadović, Aleksandra, Stanković, Anica, Debeljak Martačić, Jasmina, Ranković, Slavica, Kovačević, Sanjin, Nešović Ostojić, Jelena, Ilić, Anđelija, Milašin, Jelena, De Luka, Silvio, Trbovich, Alexander M., "Liver phospholipid fatty acid composition in response to chronic high-fat diets" in Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1869, no. 7 (2024):159526, https://doi.org/10.1016/j.bbalip.2024.159526 . .