The Polymorphisms in GSTO Genes (GSTO1 rs4925, GSTO2 rs156697, and GSTO2 rs2297235) Affect the Risk for Testicular Germ Cell Tumor Development: A Pilot Study
Authors
Petrović, MilošSimić, Tatjana
Đukić, Tatjana
Radić, Tanja
Savić-Radojević, Ana
Zeković, Milica
Durutović, Otaš
Janičić, Aleksandar
Milojević, Bogomir
Kajmaković, Boris
Živković, Marko
Bojanić, Nebojša
Bumbaširević, Uroš
Ćorić, Vesna
Article (Published version)
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Members of the omega class of glutathione transferases (GSTs), GSTO1, and GSTO2, catalyze a range of reduction reactions as a part of the antioxidant defense system. Polymorphisms of genes encoding antioxidant proteins and the resultant altered redox profile have already been associated with the increased risk for testicular germ cell cancer (GCT) development. The aim of this pilot study was to assess the individual, combined, haplotype, and cumulative effect of GSTO1rs4925, GSTO2rs156697, and GSTO2rs2297235 polymorphisms with the risk for testicular GCT development, in 88 patients and 96 matched controls, through logistic regression models. We found that carriers of the GSTO1*C/A*C/C genotype exhibited an increased risk for testicular GCT development. Significant association with increased risk of testicular GCT was observed in carriers of GSTO2rs2297235*A/G*G/G genotype, and in carriers of combined GSTO2rs156697*A/G*G/G and GSTO2rs2297235*A/G*G/G genotypes. Haplotype H7 (GSTO1rs4925*...C/GSTO2rs2297235*G/GSTO2rs156697*G) exhibited higher risk of testicular GCT, however, without significant association (p > 0.05). Finally, 51% of testicular GCT patients were the carriers of all three risk-associated genotypes, with 2.5-fold increased cumulative risk. In conclusion, the results of this pilot study suggest that GSTO polymorphisms might affect the protective antioxidant activity of GSTO isoenzymes, therefore predisposing susceptible individuals toward higher risk for testicular GCT development.
Keywords:
testicular GCT / glutathione transferase / GSTO / gene polymorphism / oxidative stressSource:
Life, 2023, 13, 6, 1269-Publisher:
- Multidisciplinary Digital Publishing Institute (MDPI)
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200110 (University of Belgrade, Faculty of Medicine) (RS-MESTD-inst-2020-200110)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200015 (University of Belgrade, Institute for Medical Research) (RS-MESTD-inst-2020-200015)
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Institut za medicinska istraživanjaTY - JOUR AU - Petrović, Miloš AU - Simić, Tatjana AU - Đukić, Tatjana AU - Radić, Tanja AU - Savić-Radojević, Ana AU - Zeković, Milica AU - Durutović, Otaš AU - Janičić, Aleksandar AU - Milojević, Bogomir AU - Kajmaković, Boris AU - Živković, Marko AU - Bojanić, Nebojša AU - Bumbaširević, Uroš AU - Ćorić, Vesna PY - 2023 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1304 AB - Members of the omega class of glutathione transferases (GSTs), GSTO1, and GSTO2, catalyze a range of reduction reactions as a part of the antioxidant defense system. Polymorphisms of genes encoding antioxidant proteins and the resultant altered redox profile have already been associated with the increased risk for testicular germ cell cancer (GCT) development. The aim of this pilot study was to assess the individual, combined, haplotype, and cumulative effect of GSTO1rs4925, GSTO2rs156697, and GSTO2rs2297235 polymorphisms with the risk for testicular GCT development, in 88 patients and 96 matched controls, through logistic regression models. We found that carriers of the GSTO1*C/A*C/C genotype exhibited an increased risk for testicular GCT development. Significant association with increased risk of testicular GCT was observed in carriers of GSTO2rs2297235*A/G*G/G genotype, and in carriers of combined GSTO2rs156697*A/G*G/G and GSTO2rs2297235*A/G*G/G genotypes. Haplotype H7 (GSTO1rs4925*C/GSTO2rs2297235*G/GSTO2rs156697*G) exhibited higher risk of testicular GCT, however, without significant association (p > 0.05). Finally, 51% of testicular GCT patients were the carriers of all three risk-associated genotypes, with 2.5-fold increased cumulative risk. In conclusion, the results of this pilot study suggest that GSTO polymorphisms might affect the protective antioxidant activity of GSTO isoenzymes, therefore predisposing susceptible individuals toward higher risk for testicular GCT development. PB - Multidisciplinary Digital Publishing Institute (MDPI) T2 - Life T1 - The Polymorphisms in GSTO Genes (GSTO1 rs4925, GSTO2 rs156697, and GSTO2 rs2297235) Affect the Risk for Testicular Germ Cell Tumor Development: A Pilot Study IS - 6 SP - 1269 VL - 13 DO - 10.3390/life13061269 ER -
@article{ author = "Petrović, Miloš and Simić, Tatjana and Đukić, Tatjana and Radić, Tanja and Savić-Radojević, Ana and Zeković, Milica and Durutović, Otaš and Janičić, Aleksandar and Milojević, Bogomir and Kajmaković, Boris and Živković, Marko and Bojanić, Nebojša and Bumbaširević, Uroš and Ćorić, Vesna", year = "2023", abstract = "Members of the omega class of glutathione transferases (GSTs), GSTO1, and GSTO2, catalyze a range of reduction reactions as a part of the antioxidant defense system. Polymorphisms of genes encoding antioxidant proteins and the resultant altered redox profile have already been associated with the increased risk for testicular germ cell cancer (GCT) development. The aim of this pilot study was to assess the individual, combined, haplotype, and cumulative effect of GSTO1rs4925, GSTO2rs156697, and GSTO2rs2297235 polymorphisms with the risk for testicular GCT development, in 88 patients and 96 matched controls, through logistic regression models. We found that carriers of the GSTO1*C/A*C/C genotype exhibited an increased risk for testicular GCT development. Significant association with increased risk of testicular GCT was observed in carriers of GSTO2rs2297235*A/G*G/G genotype, and in carriers of combined GSTO2rs156697*A/G*G/G and GSTO2rs2297235*A/G*G/G genotypes. Haplotype H7 (GSTO1rs4925*C/GSTO2rs2297235*G/GSTO2rs156697*G) exhibited higher risk of testicular GCT, however, without significant association (p > 0.05). Finally, 51% of testicular GCT patients were the carriers of all three risk-associated genotypes, with 2.5-fold increased cumulative risk. In conclusion, the results of this pilot study suggest that GSTO polymorphisms might affect the protective antioxidant activity of GSTO isoenzymes, therefore predisposing susceptible individuals toward higher risk for testicular GCT development.", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", journal = "Life", title = "The Polymorphisms in GSTO Genes (GSTO1 rs4925, GSTO2 rs156697, and GSTO2 rs2297235) Affect the Risk for Testicular Germ Cell Tumor Development: A Pilot Study", number = "6", pages = "1269", volume = "13", doi = "10.3390/life13061269" }
Petrović, M., Simić, T., Đukić, T., Radić, T., Savić-Radojević, A., Zeković, M., Durutović, O., Janičić, A., Milojević, B., Kajmaković, B., Živković, M., Bojanić, N., Bumbaširević, U.,& Ćorić, V.. (2023). The Polymorphisms in GSTO Genes (GSTO1 rs4925, GSTO2 rs156697, and GSTO2 rs2297235) Affect the Risk for Testicular Germ Cell Tumor Development: A Pilot Study. in Life Multidisciplinary Digital Publishing Institute (MDPI)., 13(6), 1269. https://doi.org/10.3390/life13061269
Petrović M, Simić T, Đukić T, Radić T, Savić-Radojević A, Zeković M, Durutović O, Janičić A, Milojević B, Kajmaković B, Živković M, Bojanić N, Bumbaširević U, Ćorić V. The Polymorphisms in GSTO Genes (GSTO1 rs4925, GSTO2 rs156697, and GSTO2 rs2297235) Affect the Risk for Testicular Germ Cell Tumor Development: A Pilot Study. in Life. 2023;13(6):1269. doi:10.3390/life13061269 .
Petrović, Miloš, Simić, Tatjana, Đukić, Tatjana, Radić, Tanja, Savić-Radojević, Ana, Zeković, Milica, Durutović, Otaš, Janičić, Aleksandar, Milojević, Bogomir, Kajmaković, Boris, Živković, Marko, Bojanić, Nebojša, Bumbaširević, Uroš, Ćorić, Vesna, "The Polymorphisms in GSTO Genes (GSTO1 rs4925, GSTO2 rs156697, and GSTO2 rs2297235) Affect the Risk for Testicular Germ Cell Tumor Development: A Pilot Study" in Life, 13, no. 6 (2023):1269, https://doi.org/10.3390/life13061269 . .