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Controlled-rate versus uncontrolled-rate freezing as predictors for platelet cryopreservation efficacy
dc.creator | Balint, Bela | |
dc.creator | Paunović, Dunja | |
dc.creator | Vucetić, D | |
dc.creator | Vojvodić, Danilo | |
dc.creator | Petakov, Marijana | |
dc.creator | Trkuljić, M | |
dc.creator | Stojanović, N. | |
dc.date.accessioned | 2021-04-20T12:10:05Z | |
dc.date.available | 2021-04-20T12:10:05Z | |
dc.date.issued | 2006 | |
dc.identifier.issn | 0041-1132 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/129 | |
dc.description.abstract | BACKGROUND: Cryobiologic variables responsible for cell injuries and freezing techniques applicable in medical cryopractice should be revised and/or reengineered for minimizing cryoinjuries and maximizing cell recovery. In this study, the efficacy of different cryopreservation protocols based on platelet (PLT) recovery was evaluated. STUDY DESIGN AND METHODS: PLTs (n = 33) were prepared from whole-blood units. Cell count and viability, PLT morphologic score (PMS), and hypotonic shock response were determined. PLT surface antigens were measured by flow cytometry. Controlled-rate (with compensated fusion heat) and uncontrolled-rate freezing methods combined with 6 percent dimethyl sulfoxide were used. RESULTS: PLT recovery was superior in the controlled-rate setting (91.0 +/- 5.5 vs. 86.0 +/- 6.5; p lt 0.05). PMS was significantly better in controlled-rate freezing (p lt 0.01). GPIb/CD42b expression was reduced in both freezing groups versus control. GP140/CD62p expression was significantly (p lt 0.05) lower in the controlled-rate group and in both frozen groups was significantly higher than in the control groups. CONCLUSION: The use of strictly equalized (1 degrees C/min) controlled-rate freezing, combined with an intensified cooling rate (2 degrees C/min) during the liquid-to-solid-phase transition period, allows advanced quantitative and qualitative PLT recovery, even though the minor intergroup differences for some variables were observed. | en |
dc.publisher | Wiley, Hoboken | |
dc.rights | restrictedAccess | |
dc.source | Transfusion | |
dc.title | Controlled-rate versus uncontrolled-rate freezing as predictors for platelet cryopreservation efficacy | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 235 | |
dc.citation.issue | 2 | |
dc.citation.other | 46(2): 230-235 | |
dc.citation.rank | M22 | |
dc.citation.spage | 230 | |
dc.citation.volume | 46 | |
dc.identifier.doi | 10.1111/j.1537-2995.2006.00706.x | |
dc.identifier.pmid | 16441600 | |
dc.identifier.scopus | 2-s2.0-33644864194 | |
dc.identifier.wos | 000234856000013 | |
dc.type.version | publishedVersion |