Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology
Authors
Vajić, Una JovanaŽivković, Jelena
Ivanov, Milan
Jovović, Đurđica
Šavikin, Katarina
Bugarski, Branko
Mihailović-Stanojević, Nevena
Article (Published version)
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The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolicextracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75and 100%) and extraction time (30, 60and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69% aqueous methanol and 67 min..., and 65% aqueous methanol and 83 min for maximal FRAPantioxidantcapacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.
Keywords:
Urtica dioica L. / flavonoids / antioxidant capacity / phenolic compounds / response surface methodologySource:
Macedonian Journal of Chemistry and Chemical Engineering, 2022, 41, 1, 119-128Publisher:
- Society of Chemists and Technologists of Macedonia and Ss. Cyril and Methodius University in Skopje
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200003 (Institute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade) (RS-200003)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200015 (University of Belgrade, Institute for Medical Research) (RS-200015)
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Institution/Community
Institut za medicinska istraživanjaTY - JOUR AU - Vajić, Una Jovana AU - Živković, Jelena AU - Ivanov, Milan AU - Jovović, Đurđica AU - Šavikin, Katarina AU - Bugarski, Branko AU - Mihailović-Stanojević, Nevena PY - 2022 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1269 AB - The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolicextracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75and 100%) and extraction time (30, 60and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69% aqueous methanol and 67 min, and 65% aqueous methanol and 83 min for maximal FRAPantioxidantcapacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent. PB - Society of Chemists and Technologists of Macedonia and Ss. Cyril and Methodius University in Skopje T2 - Macedonian Journal of Chemistry and Chemical Engineering T1 - Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology EP - 128 IS - 1 SP - 119 VL - 41 DO - 10.20450/mjcce.2022.2238 ER -
@article{ author = "Vajić, Una Jovana and Živković, Jelena and Ivanov, Milan and Jovović, Đurđica and Šavikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena", year = "2022", abstract = "The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolicextracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75and 100%) and extraction time (30, 60and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69% aqueous methanol and 67 min, and 65% aqueous methanol and 83 min for maximal FRAPantioxidantcapacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.", publisher = "Society of Chemists and Technologists of Macedonia and Ss. Cyril and Methodius University in Skopje", journal = "Macedonian Journal of Chemistry and Chemical Engineering", title = "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology", pages = "128-119", number = "1", volume = "41", doi = "10.20450/mjcce.2022.2238" }
Vajić, U. J., Živković, J., Ivanov, M., Jovović, Đ., Šavikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2022). Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering Society of Chemists and Technologists of Macedonia and Ss. Cyril and Methodius University in Skopje., 41(1), 119-128. https://doi.org/10.20450/mjcce.2022.2238
Vajić UJ, Živković J, Ivanov M, Jovović Đ, Šavikin K, Bugarski B, Mihailović-Stanojević N. Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering. 2022;41(1):119-128. doi:10.20450/mjcce.2022.2238 .
Vajić, Una Jovana, Živković, Jelena, Ivanov, Milan, Jovović, Đurđica, Šavikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology" in Macedonian Journal of Chemistry and Chemical Engineering, 41, no. 1 (2022):119-128, https://doi.org/10.20450/mjcce.2022.2238 . .