Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange
Authors
Uzelac, AleksandraKlun, Ivana
Ćirković, Vladimir
Bauman, Neda
Bobić, Branko
Štajner, Tijana
Srbljanović, Jelena
Lijeskić, Olivera
Đurković-Đaković, Olgica
Article (Published version)
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In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of lineage III genotypes varies geographically and lineage I are rare, yet present in several regions of the continent. Data on the T. gondii population structure in southeastern Europe (SEE) are scarce, yet necessary to appreciate the diversity of the species in Europe. To help fill this gap, we genotyped 67 strains from nine species of intermediate hosts in Serbia by MnPCR-RFLP, determined the population structure, and identified the genotypes using ToxoDB. A neighbor-joining tree was also constructed from the isolates genotyped on nine loci. While 42% of the total genotype population consisted of ToxoDB#1 and ToxoDB#2, variant genotypes of both lineages comprised 46% of the population in wildlife and 28% in domestic animals and humans. One genotype of Africa 4 lineage was detected in a human sample. Interestingly, the findings include one lineage III variant and on...e II/III recombinant isolate with intercontinental distribution, which appear to be moderately related to South American genotypes. Based on these findings, SEE is a region of underappreciated T. gondii genetic diversity and possible strain exchange between Europe and Africa.
Keywords:
Toxoplasma gondii / Africa / Europe / genotypes / population / structureSource:
Microorganisms, 2021, 9, 12, 2526-Publisher:
- MDPI
Funding / projects:
- Control of infections by Apicomplexan pathogens: from novel drug targets to prediction (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41019)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200015 (University of Belgrade, Institute for Medical Research) (RS-MESTD-inst-2020-200015)
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Institut za medicinska istraživanjaTY - JOUR AU - Uzelac, Aleksandra AU - Klun, Ivana AU - Ćirković, Vladimir AU - Bauman, Neda AU - Bobić, Branko AU - Štajner, Tijana AU - Srbljanović, Jelena AU - Lijeskić, Olivera AU - Đurković-Đaković, Olgica PY - 2021 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1190 AB - In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of lineage III genotypes varies geographically and lineage I are rare, yet present in several regions of the continent. Data on the T. gondii population structure in southeastern Europe (SEE) are scarce, yet necessary to appreciate the diversity of the species in Europe. To help fill this gap, we genotyped 67 strains from nine species of intermediate hosts in Serbia by MnPCR-RFLP, determined the population structure, and identified the genotypes using ToxoDB. A neighbor-joining tree was also constructed from the isolates genotyped on nine loci. While 42% of the total genotype population consisted of ToxoDB#1 and ToxoDB#2, variant genotypes of both lineages comprised 46% of the population in wildlife and 28% in domestic animals and humans. One genotype of Africa 4 lineage was detected in a human sample. Interestingly, the findings include one lineage III variant and one II/III recombinant isolate with intercontinental distribution, which appear to be moderately related to South American genotypes. Based on these findings, SEE is a region of underappreciated T. gondii genetic diversity and possible strain exchange between Europe and Africa. PB - MDPI T2 - Microorganisms T1 - Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange IS - 12 SP - 2526 VL - 9 DO - 10.3390/microorganisms9122526 ER -
@article{ author = "Uzelac, Aleksandra and Klun, Ivana and Ćirković, Vladimir and Bauman, Neda and Bobić, Branko and Štajner, Tijana and Srbljanović, Jelena and Lijeskić, Olivera and Đurković-Đaković, Olgica", year = "2021", abstract = "In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of lineage III genotypes varies geographically and lineage I are rare, yet present in several regions of the continent. Data on the T. gondii population structure in southeastern Europe (SEE) are scarce, yet necessary to appreciate the diversity of the species in Europe. To help fill this gap, we genotyped 67 strains from nine species of intermediate hosts in Serbia by MnPCR-RFLP, determined the population structure, and identified the genotypes using ToxoDB. A neighbor-joining tree was also constructed from the isolates genotyped on nine loci. While 42% of the total genotype population consisted of ToxoDB#1 and ToxoDB#2, variant genotypes of both lineages comprised 46% of the population in wildlife and 28% in domestic animals and humans. One genotype of Africa 4 lineage was detected in a human sample. Interestingly, the findings include one lineage III variant and one II/III recombinant isolate with intercontinental distribution, which appear to be moderately related to South American genotypes. Based on these findings, SEE is a region of underappreciated T. gondii genetic diversity and possible strain exchange between Europe and Africa.", publisher = "MDPI", journal = "Microorganisms", title = "Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange", number = "12", pages = "2526", volume = "9", doi = "10.3390/microorganisms9122526" }
Uzelac, A., Klun, I., Ćirković, V., Bauman, N., Bobić, B., Štajner, T., Srbljanović, J., Lijeskić, O.,& Đurković-Đaković, O.. (2021). Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange. in Microorganisms MDPI., 9(12), 2526. https://doi.org/10.3390/microorganisms9122526
Uzelac A, Klun I, Ćirković V, Bauman N, Bobić B, Štajner T, Srbljanović J, Lijeskić O, Đurković-Đaković O. Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange. in Microorganisms. 2021;9(12):2526. doi:10.3390/microorganisms9122526 .
Uzelac, Aleksandra, Klun, Ivana, Ćirković, Vladimir, Bauman, Neda, Bobić, Branko, Štajner, Tijana, Srbljanović, Jelena, Lijeskić, Olivera, Đurković-Đaković, Olgica, "Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange" in Microorganisms, 9, no. 12 (2021):2526, https://doi.org/10.3390/microorganisms9122526 . .