Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects
Autori
Paraš, SmiljanaTrišić, Dijana
Mitrović-Ajtić, Olivera
Antonijević, Đorđe
Čolović, Božana
Drobne, Damjana
Jokanović, Vukoman
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The effects of a new material based on hydroxyapatite and calcium silicates, named ALBO-MPCA, were investigated on the liver, kidney and spleen. The material was administrated orally for 120 days in an in vivo model in Wistar rats, and untreated animals served as a control. Hematological and biochemical blood parameters were analyzed. Qualitative histological analysis of tissues, change in mitotic activity of cells, and histological characteristics was conducted, as well as quantitative stereological analysis of parenchymal cells, blood sinusoids, and connective tissues. Additionally, the protein expressions of Ki67 and CD68 markers were evaluated. Histological analysis revealed no pathological changes after the tested period. It showed the preservation of the architecture of blood sinusoids and epithelial cells and the presence of mitosis. Additionally, the significantly increased number of the Ki67 in the presence of ALBO-MPCA confirmed the proliferative effect of the material notice...d by stereological analysis, while immunoreactive CD68 positive cells did not differ between groups. The study showed non-toxicity of the tested material based on the effects on the hematological, biochemical, and observed histological parameters; in addition, it showed evidence of its biocompatibility. These results could be the basis for further steps toward the application of tested materials in endodontics.
Ključne reči:
biocompatibility / calcium silicates / dental cement / hydroxyapatite / subchronic toxicityIzvor:
International Journal of Molecular Science, 2021, 22, 11, 5468-Izdavač:
- MDPI
Finansiranje / projekti:
- Hemijsko i strukturno dizajniranje nanomaterijala za primenu u medicini i inženjerstvu tkiva (RS-MESTD-Basic Research (BR or ON)-172026)
- Funkcionalni, funkcionalizovani i usavršeni nano materijali (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45005)
- Javna Agencija za Raziskovalno Dejavnost RS J1-9162,P1-0207
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Paraš, Smiljana AU - Trišić, Dijana AU - Mitrović-Ajtić, Olivera AU - Antonijević, Đorđe AU - Čolović, Božana AU - Drobne, Damjana AU - Jokanović, Vukoman PY - 2021 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1134 AB - The effects of a new material based on hydroxyapatite and calcium silicates, named ALBO-MPCA, were investigated on the liver, kidney and spleen. The material was administrated orally for 120 days in an in vivo model in Wistar rats, and untreated animals served as a control. Hematological and biochemical blood parameters were analyzed. Qualitative histological analysis of tissues, change in mitotic activity of cells, and histological characteristics was conducted, as well as quantitative stereological analysis of parenchymal cells, blood sinusoids, and connective tissues. Additionally, the protein expressions of Ki67 and CD68 markers were evaluated. Histological analysis revealed no pathological changes after the tested period. It showed the preservation of the architecture of blood sinusoids and epithelial cells and the presence of mitosis. Additionally, the significantly increased number of the Ki67 in the presence of ALBO-MPCA confirmed the proliferative effect of the material noticed by stereological analysis, while immunoreactive CD68 positive cells did not differ between groups. The study showed non-toxicity of the tested material based on the effects on the hematological, biochemical, and observed histological parameters; in addition, it showed evidence of its biocompatibility. These results could be the basis for further steps toward the application of tested materials in endodontics. PB - MDPI T2 - International Journal of Molecular Science T1 - Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects IS - 11 SP - 5468 VL - 22 DO - 10.3390/ijms22115468 ER -
@article{ author = "Paraš, Smiljana and Trišić, Dijana and Mitrović-Ajtić, Olivera and Antonijević, Đorđe and Čolović, Božana and Drobne, Damjana and Jokanović, Vukoman", year = "2021", abstract = "The effects of a new material based on hydroxyapatite and calcium silicates, named ALBO-MPCA, were investigated on the liver, kidney and spleen. The material was administrated orally for 120 days in an in vivo model in Wistar rats, and untreated animals served as a control. Hematological and biochemical blood parameters were analyzed. Qualitative histological analysis of tissues, change in mitotic activity of cells, and histological characteristics was conducted, as well as quantitative stereological analysis of parenchymal cells, blood sinusoids, and connective tissues. Additionally, the protein expressions of Ki67 and CD68 markers were evaluated. Histological analysis revealed no pathological changes after the tested period. It showed the preservation of the architecture of blood sinusoids and epithelial cells and the presence of mitosis. Additionally, the significantly increased number of the Ki67 in the presence of ALBO-MPCA confirmed the proliferative effect of the material noticed by stereological analysis, while immunoreactive CD68 positive cells did not differ between groups. The study showed non-toxicity of the tested material based on the effects on the hematological, biochemical, and observed histological parameters; in addition, it showed evidence of its biocompatibility. These results could be the basis for further steps toward the application of tested materials in endodontics.", publisher = "MDPI", journal = "International Journal of Molecular Science", title = "Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects", number = "11", pages = "5468", volume = "22", doi = "10.3390/ijms22115468" }
Paraš, S., Trišić, D., Mitrović-Ajtić, O., Antonijević, Đ., Čolović, B., Drobne, D.,& Jokanović, V.. (2021). Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects. in International Journal of Molecular Science MDPI., 22(11), 5468. https://doi.org/10.3390/ijms22115468
Paraš S, Trišić D, Mitrović-Ajtić O, Antonijević Đ, Čolović B, Drobne D, Jokanović V. Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects. in International Journal of Molecular Science. 2021;22(11):5468. doi:10.3390/ijms22115468 .
Paraš, Smiljana, Trišić, Dijana, Mitrović-Ajtić, Olivera, Antonijević, Đorđe, Čolović, Božana, Drobne, Damjana, Jokanović, Vukoman, "Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects" in International Journal of Molecular Science, 22, no. 11 (2021):5468, https://doi.org/10.3390/ijms22115468 . .