dc.creator | Obradović, Hristina | |
dc.creator | Krstić, Jelena | |
dc.creator | Trivanović, Drenka | |
dc.creator | Mojsilović, Slavko | |
dc.creator | Okić, Ivana | |
dc.creator | Kukolj, Tamara | |
dc.creator | Ilić, Vesna | |
dc.creator | Jauković, Aleksandra | |
dc.creator | Terzić, Milan | |
dc.creator | Bugarski, Diana | |
dc.date.accessioned | 2021-04-20T13:04:36Z | |
dc.date.available | 2021-04-20T13:04:36Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0143-4004 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/969 | |
dc.description.abstract | Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use. | en |
dc.publisher | W B Saunders Co Ltd, London | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS// | |
dc.rights | restrictedAccess | |
dc.source | Placenta | |
dc.subject | Mesenchymal stem cells (MSC) | en |
dc.subject | Wharton's jelly | en |
dc.subject | Hypoxia | en |
dc.subject | Stemness | en |
dc.subject | Migration | en |
dc.title | Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 34 | |
dc.citation.other | 82: 25-34 | |
dc.citation.rank | M21 | |
dc.citation.spage | 25 | |
dc.citation.volume | 82 | |
dc.identifier.doi | 10.1016/j.placenta.2019.05.005 | |
dc.identifier.pmid | 31174623 | |
dc.identifier.scopus | 2-s2.0-85065744920 | |
dc.identifier.wos | 000470100100005 | |
dc.type.version | publishedVersion | |