beta-catenin and PPAR-gamma levels in bone marrow of myeloproliferative neoplasm: an immunohistochemical and ultrastructural study
Нема приказа
Аутори
Subotički, TijanaMitrović-Ajtić, Olivera
Mićić, Mileva
Kravic-Stevović, Tamara
Đikić, Dragoslava
Diklić, Miloš
Leković, Danijela
Gotić, Mirjana
Čokić, Vladan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In accordance with increased proliferation in myeloproliferative neoplasm (MPN), the goal is to evaluate the immunoexpression of: beta-catenin, PPAR-gamma and Ki67 protein, to compare them with bone marrow ultrastructural characteristics in patients with MPN. Immunoexpression and electron microscopy of bone marrow was analyzed in 30 Ph-negative MPN patients, including per 10 patients with polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF). The quantity of beta-catenin immunoreactive cells was significantly higher in PV then in ET (p lt 0.01) or PMF group of patients (p lt 0.01) and also in ET versus PMF group of patients (p lt 0.01). Erythroid lineage showed absent beta-catenin staining without immunoreactivity in nucleus. In contrast, immunoreactivity for PPAR-gamma was localized mostly in megakaryocytes and the highest number of PPAR-gamma immunopositive cells was detected in PMF group of patients. In addition, the proliferative Ki67 index wa...s significantly increased in the PMF and PV patients compared to patients with ET. Also, the megakaryocytes showed abnormal maturation in PMF group of patients as determined by ultrastructural analysis. These results indicated that PV dominantly expressed beta-catenin and proliferation marker Ki67 in bone marrow, while PMF is linked preferentially to PPAR-gamma immunopositive megakaryocytes characterized by abnormal maturation.
Кључне речи:
Myeloproliferative neoplasm / beta-catenin / PPAR gamma / Ki67Извор:
Ultrastructural Pathology, 2018, 42, 6, 498-507Издавач:
- Taylor & Francis Inc, Philadelphia
Финансирање / пројекти:
- Испитивање патогенезе хематолошких малигнитета (RS-MESTD-Basic Research (BR or ON)-175053)
- Swiss National Science Foundation through Joint research project (SCOPES) [IZ73Z0 152420/1]
DOI: 10.1080/01913123.2018.1558323
ISSN: 0191-3123
PubMed: 30582392
WoS: 000457548500004
Scopus: 2-s2.0-85059053031
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Subotički, Tijana AU - Mitrović-Ajtić, Olivera AU - Mićić, Mileva AU - Kravic-Stevović, Tamara AU - Đikić, Dragoslava AU - Diklić, Miloš AU - Leković, Danijela AU - Gotić, Mirjana AU - Čokić, Vladan PY - 2018 UR - http://rimi.imi.bg.ac.rs/handle/123456789/853 AB - In accordance with increased proliferation in myeloproliferative neoplasm (MPN), the goal is to evaluate the immunoexpression of: beta-catenin, PPAR-gamma and Ki67 protein, to compare them with bone marrow ultrastructural characteristics in patients with MPN. Immunoexpression and electron microscopy of bone marrow was analyzed in 30 Ph-negative MPN patients, including per 10 patients with polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF). The quantity of beta-catenin immunoreactive cells was significantly higher in PV then in ET (p lt 0.01) or PMF group of patients (p lt 0.01) and also in ET versus PMF group of patients (p lt 0.01). Erythroid lineage showed absent beta-catenin staining without immunoreactivity in nucleus. In contrast, immunoreactivity for PPAR-gamma was localized mostly in megakaryocytes and the highest number of PPAR-gamma immunopositive cells was detected in PMF group of patients. In addition, the proliferative Ki67 index was significantly increased in the PMF and PV patients compared to patients with ET. Also, the megakaryocytes showed abnormal maturation in PMF group of patients as determined by ultrastructural analysis. These results indicated that PV dominantly expressed beta-catenin and proliferation marker Ki67 in bone marrow, while PMF is linked preferentially to PPAR-gamma immunopositive megakaryocytes characterized by abnormal maturation. PB - Taylor & Francis Inc, Philadelphia T2 - Ultrastructural Pathology T1 - beta-catenin and PPAR-gamma levels in bone marrow of myeloproliferative neoplasm: an immunohistochemical and ultrastructural study EP - 507 IS - 6 SP - 498 VL - 42 DO - 10.1080/01913123.2018.1558323 ER -
@article{ author = "Subotički, Tijana and Mitrović-Ajtić, Olivera and Mićić, Mileva and Kravic-Stevović, Tamara and Đikić, Dragoslava and Diklić, Miloš and Leković, Danijela and Gotić, Mirjana and Čokić, Vladan", year = "2018", abstract = "In accordance with increased proliferation in myeloproliferative neoplasm (MPN), the goal is to evaluate the immunoexpression of: beta-catenin, PPAR-gamma and Ki67 protein, to compare them with bone marrow ultrastructural characteristics in patients with MPN. Immunoexpression and electron microscopy of bone marrow was analyzed in 30 Ph-negative MPN patients, including per 10 patients with polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF). The quantity of beta-catenin immunoreactive cells was significantly higher in PV then in ET (p lt 0.01) or PMF group of patients (p lt 0.01) and also in ET versus PMF group of patients (p lt 0.01). Erythroid lineage showed absent beta-catenin staining without immunoreactivity in nucleus. In contrast, immunoreactivity for PPAR-gamma was localized mostly in megakaryocytes and the highest number of PPAR-gamma immunopositive cells was detected in PMF group of patients. In addition, the proliferative Ki67 index was significantly increased in the PMF and PV patients compared to patients with ET. Also, the megakaryocytes showed abnormal maturation in PMF group of patients as determined by ultrastructural analysis. These results indicated that PV dominantly expressed beta-catenin and proliferation marker Ki67 in bone marrow, while PMF is linked preferentially to PPAR-gamma immunopositive megakaryocytes characterized by abnormal maturation.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Ultrastructural Pathology", title = "beta-catenin and PPAR-gamma levels in bone marrow of myeloproliferative neoplasm: an immunohistochemical and ultrastructural study", pages = "507-498", number = "6", volume = "42", doi = "10.1080/01913123.2018.1558323" }
Subotički, T., Mitrović-Ajtić, O., Mićić, M., Kravic-Stevović, T., Đikić, D., Diklić, M., Leković, D., Gotić, M.,& Čokić, V.. (2018). beta-catenin and PPAR-gamma levels in bone marrow of myeloproliferative neoplasm: an immunohistochemical and ultrastructural study. in Ultrastructural Pathology Taylor & Francis Inc, Philadelphia., 42(6), 498-507. https://doi.org/10.1080/01913123.2018.1558323
Subotički T, Mitrović-Ajtić O, Mićić M, Kravic-Stevović T, Đikić D, Diklić M, Leković D, Gotić M, Čokić V. beta-catenin and PPAR-gamma levels in bone marrow of myeloproliferative neoplasm: an immunohistochemical and ultrastructural study. in Ultrastructural Pathology. 2018;42(6):498-507. doi:10.1080/01913123.2018.1558323 .
Subotički, Tijana, Mitrović-Ajtić, Olivera, Mićić, Mileva, Kravic-Stevović, Tamara, Đikić, Dragoslava, Diklić, Miloš, Leković, Danijela, Gotić, Mirjana, Čokić, Vladan, "beta-catenin and PPAR-gamma levels in bone marrow of myeloproliferative neoplasm: an immunohistochemical and ultrastructural study" in Ultrastructural Pathology, 42, no. 6 (2018):498-507, https://doi.org/10.1080/01913123.2018.1558323 . .