Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants
Само за регистроване кориснике
2017
Аутори
Jakovetić-Tanasković, SonjaJokić, Bojan
Grbavcić, Sanja
Drvenica, Ivana
Prlainović, Nevena
Luković, Nevena
Knežević-Jugović, Zorica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Lipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10 mM acetic buffer pH 5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved.
Кључне речи:
Lipase B from Candida antarctica / Kaolin / Immobilization / MetakaolinИзвор:
Applied Clay Science, 2017, 135, 103-111Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1016/j.clay.2016.09.011
ISSN: 0169-1317
WoS: 000390621800012
Scopus: 2-s2.0-84994716248
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Jakovetić-Tanasković, Sonja AU - Jokić, Bojan AU - Grbavcić, Sanja AU - Drvenica, Ivana AU - Prlainović, Nevena AU - Luković, Nevena AU - Knežević-Jugović, Zorica PY - 2017 UR - http://rimi.imi.bg.ac.rs/handle/123456789/801 AB - Lipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10 mM acetic buffer pH 5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved. PB - Elsevier Science Bv, Amsterdam T2 - Applied Clay Science T1 - Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants EP - 111 SP - 103 VL - 135 DO - 10.1016/j.clay.2016.09.011 ER -
@article{ author = "Jakovetić-Tanasković, Sonja and Jokić, Bojan and Grbavcić, Sanja and Drvenica, Ivana and Prlainović, Nevena and Luković, Nevena and Knežević-Jugović, Zorica", year = "2017", abstract = "Lipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10 mM acetic buffer pH 5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Applied Clay Science", title = "Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants", pages = "111-103", volume = "135", doi = "10.1016/j.clay.2016.09.011" }
Jakovetić-Tanasković, S., Jokić, B., Grbavcić, S., Drvenica, I., Prlainović, N., Luković, N.,& Knežević-Jugović, Z.. (2017). Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants. in Applied Clay Science Elsevier Science Bv, Amsterdam., 135, 103-111. https://doi.org/10.1016/j.clay.2016.09.011
Jakovetić-Tanasković S, Jokić B, Grbavcić S, Drvenica I, Prlainović N, Luković N, Knežević-Jugović Z. Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants. in Applied Clay Science. 2017;135:103-111. doi:10.1016/j.clay.2016.09.011 .
Jakovetić-Tanasković, Sonja, Jokić, Bojan, Grbavcić, Sanja, Drvenica, Ivana, Prlainović, Nevena, Luković, Nevena, Knežević-Jugović, Zorica, "Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants" in Applied Clay Science, 135 (2017):103-111, https://doi.org/10.1016/j.clay.2016.09.011 . .