Приказ основних података о документу
Rac1 modulates TGF-beta 1-mediated epithelial cell plasticity and MMP9 production in transformed keratinocytes
dc.creator | Santibanez, Juan F. | |
dc.creator | Krstić, Jelena | |
dc.creator | Fabra, Angels | |
dc.creator | Cano, Amparo | |
dc.creator | Quintanilla, Miguel | |
dc.date.accessioned | 2021-04-20T12:20:57Z | |
dc.date.available | 2021-04-20T12:20:57Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0014-5793 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/291 | |
dc.description.abstract | Transforming growth factor-beta 1 (TGF-beta 1) activates Rac1 GTPase in mouse transformed keratinocytes. Expression of a constitutively active Q61LRac1 mutant induced an epithelial to mesenchymal transition (EMT) linked to stimulation of cell migration and invasion. On the contrary, expression of a dominant-negative N17TRac1 abolished TGF-beta 1-induced cell scattering, migration and invasion. Moreover, Q61LRac1 enhanced metalloproteinase-9 (MMP9) production to levels comparable to those induced by TGF-beta 1, while N17TRac1 was inhibitory. TGF-beta 1-mediated EMT involves the expression of the E-cadherin repressor Snail1, regulated by the Rac1 and mitogen-activated protein kinase (MAPK) pathways. Furthermore, MMP9 production was MAPK-dependent, as the MEK inhibitor PD98059 decreased TGF-beta 1-induced MMP9 expression and secretion in Q61LRac1 expressing cells. We propose that regulation of TGF-beta 1-mediated plasticity of transformed keratinocytes requires the cooperation between the Rac1 and MAPK signalling pathways. | en |
dc.publisher | Elsevier Science Bv, Amsterdam | |
dc.relation | Fund for Science and Technology of Chile (grants FONDECYT #1050476 and # 3000045 to J.F.S.) | |
dc.relation | University of Chile (DID) [I003-99/2] | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/145048/RS// | |
dc.relation | Spanish Ministry of Science and Innovation, Spanish Government [SAF2007-63821] | |
dc.rights | openAccess | |
dc.source | FEBS Letters | |
dc.subject | Keratinocyte | en |
dc.subject | Transforming growth factor-beta 1 | en |
dc.subject | Metalloproteinase-9 | en |
dc.subject | Rac1 | en |
dc.subject | Snail1 | en |
dc.subject | Mitogen-activated protein kinase | en |
dc.subject | Epithelial-mesenchymal transition | en |
dc.subject | Migration | en |
dc.title | Rac1 modulates TGF-beta 1-mediated epithelial cell plasticity and MMP9 production in transformed keratinocytes | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 2310 | |
dc.citation.issue | 11 | |
dc.citation.other | 584(11): 2305-2310 | |
dc.citation.rank | M22 | |
dc.citation.spage | 2305 | |
dc.citation.volume | 584 | |
dc.identifier.doi | 10.1016/j.febslet.2010.03.042 | |
dc.identifier.fulltext | http://rimi.imi.bg.ac.rs/bitstream/id/235/288.pdf | |
dc.identifier.pmid | 20353788 | |
dc.identifier.scopus | 2-s2.0-77952956960 | |
dc.identifier.wos | 000277793200022 | |
dc.type.version | publishedVersion |