SKIP is required for TGF-beta 1-induced epithelial mesenchymal transition and migration in transformed keratinocytes
Апстракт
Transforming growth factor-beta 1 (TGF-beta 1) potently induces the epithelial-mesenchymal transition (EMT) during tumoral progression. Although Sky-interacting protein (SKIP) regulates TGF-beta 1-induced Smad activation, its role in the induction of cell malignance remains uncertain. We found that TGF-beta 1 increases SKIP expression in PDV cells. In cells stably transfected with SKIP antisense, AS-S, Smad3 activation decreased, along with an inhibition of TGF-beta 1-induced EMT, and the cells were sensitized to the TGF-beta 1-dependent inhibition of proliferation. Also, AS-S cells showed a weaker migration and invasion response. Moreover, TGF-beta 1-induced urokinase-type plasminogen activator expression was inhibited, concomitantly with a TGF-beta 1-independent increment of the plasminogen-activator inhibitor-1 expression. Thus, these results suggest that SKIP is required for EMT and invasiveness induced by TGF-beta 1 in transformed cells.
Кључне речи:
Transforming growth factor-beta 1 / Sky-interacting protein / Epithelial-mesenchymal transition / Urokinase type plasminogen activator / Plasminogen activator inhibitor type-1 / MigrationИзвор:
FEBS Letters, 2010, 584, 22, 4586-4592Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Chile Comision Nacional de Investigacion Cientifica y Tecnologica FONDECYT [1050476]
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-MESTD-Basic Research (BR or ON)-175062)
DOI: 10.1016/j.febslet.2010.10.020
ISSN: 1873-3468
PubMed: 20965173
WoS: 000284148000016
Scopus: 2-s2.0-78249232622
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Villar, Victor AU - Kocić, Jelena AU - Bugarski, Diana AU - Jovčić, Gordana AU - Santibanez, Juan F. PY - 2010 UR - http://rimi.imi.bg.ac.rs/handle/123456789/287 AB - Transforming growth factor-beta 1 (TGF-beta 1) potently induces the epithelial-mesenchymal transition (EMT) during tumoral progression. Although Sky-interacting protein (SKIP) regulates TGF-beta 1-induced Smad activation, its role in the induction of cell malignance remains uncertain. We found that TGF-beta 1 increases SKIP expression in PDV cells. In cells stably transfected with SKIP antisense, AS-S, Smad3 activation decreased, along with an inhibition of TGF-beta 1-induced EMT, and the cells were sensitized to the TGF-beta 1-dependent inhibition of proliferation. Also, AS-S cells showed a weaker migration and invasion response. Moreover, TGF-beta 1-induced urokinase-type plasminogen activator expression was inhibited, concomitantly with a TGF-beta 1-independent increment of the plasminogen-activator inhibitor-1 expression. Thus, these results suggest that SKIP is required for EMT and invasiveness induced by TGF-beta 1 in transformed cells. PB - Wiley, Hoboken T2 - FEBS Letters T1 - SKIP is required for TGF-beta 1-induced epithelial mesenchymal transition and migration in transformed keratinocytes EP - 4592 IS - 22 SP - 4586 VL - 584 DO - 10.1016/j.febslet.2010.10.020 ER -
@article{ author = "Villar, Victor and Kocić, Jelena and Bugarski, Diana and Jovčić, Gordana and Santibanez, Juan F.", year = "2010", abstract = "Transforming growth factor-beta 1 (TGF-beta 1) potently induces the epithelial-mesenchymal transition (EMT) during tumoral progression. Although Sky-interacting protein (SKIP) regulates TGF-beta 1-induced Smad activation, its role in the induction of cell malignance remains uncertain. We found that TGF-beta 1 increases SKIP expression in PDV cells. In cells stably transfected with SKIP antisense, AS-S, Smad3 activation decreased, along with an inhibition of TGF-beta 1-induced EMT, and the cells were sensitized to the TGF-beta 1-dependent inhibition of proliferation. Also, AS-S cells showed a weaker migration and invasion response. Moreover, TGF-beta 1-induced urokinase-type plasminogen activator expression was inhibited, concomitantly with a TGF-beta 1-independent increment of the plasminogen-activator inhibitor-1 expression. Thus, these results suggest that SKIP is required for EMT and invasiveness induced by TGF-beta 1 in transformed cells.", publisher = "Wiley, Hoboken", journal = "FEBS Letters", title = "SKIP is required for TGF-beta 1-induced epithelial mesenchymal transition and migration in transformed keratinocytes", pages = "4592-4586", number = "22", volume = "584", doi = "10.1016/j.febslet.2010.10.020" }
Villar, V., Kocić, J., Bugarski, D., Jovčić, G.,& Santibanez, J. F.. (2010). SKIP is required for TGF-beta 1-induced epithelial mesenchymal transition and migration in transformed keratinocytes. in FEBS Letters Wiley, Hoboken., 584(22), 4586-4592. https://doi.org/10.1016/j.febslet.2010.10.020
Villar V, Kocić J, Bugarski D, Jovčić G, Santibanez JF. SKIP is required for TGF-beta 1-induced epithelial mesenchymal transition and migration in transformed keratinocytes. in FEBS Letters. 2010;584(22):4586-4592. doi:10.1016/j.febslet.2010.10.020 .
Villar, Victor, Kocić, Jelena, Bugarski, Diana, Jovčić, Gordana, Santibanez, Juan F., "SKIP is required for TGF-beta 1-induced epithelial mesenchymal transition and migration in transformed keratinocytes" in FEBS Letters, 584, no. 22 (2010):4586-4592, https://doi.org/10.1016/j.febslet.2010.10.020 . .