Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Francisella tularensis and their co-infections in host-seeking Ixodes ricinus ticks collected in Serbia
Само за регистроване кориснике
2008
Аутори
Milutinović, MarijaMasuzawa, Toshiyuki
Tomanović, Snežana
Radulović, Željko
Fukui, Takashi
Okamoto, Yoshihiro
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
To evaluate the prevalence rate of tick-borne bacterial pathogens, unfed adult Ixodes ricinus ticks were collected from vegetation in 2001, 2003, and 2004 at 18 localities throughout Serbia. A total of 287 ticks were examined by PCR technique for the presence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and Francisella tularensis. The highest prevalence rate was that for B. burgdorferi sensu lato (42.5%), followed by A. phagocytophilum (13.9%) and F. tularensis (3.8%). The presence of five B. burgdorferi sensu lato genospecies, namely, B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. lusitaniae, and B. valaisiana was identified by restriction fragment length polymorphism (RFLP) analysis. The most frequent B. burgdorferi sensu lato genospecies was B. lusitaniae, followed by B. burgdorferi sensu stricto. Co-infection by B. burgdorferi sensu stricto and B. lusitaniae was frequently observed. Co-infection by B. burgdorferi sensu lato and A. phagocytophilum and co-...infection by B. burgdorferi sensu lato and F. tularensis appeared in 24 ticks. Sequencing of p44/msp2 paralogs of Serbian A. phagocytophilum showed that they were unique and distinct from those of A. phagocytophilum in US and UK. This is the first report of B. garinii, B. lusitaniae, B. valaisiana, as well as A. phagocytophilum and F. tularensis infected ticks in Serbia. These findings indicate a public health threat in Serbia of tick-borne diseases caused by B. burgdorferi sensu lato, A. phagocytophilum and F. tularensis.
Кључне речи:
Anaplasma phagocytophilum / Borrelia burgdorferi / Francisella tularensis / Ixodes ricinusИзвор:
Experimental & Applied Acarology, 2008, 45, 3-4, 171-183Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Chiba Institute of Science (CIS)
- Инфекције интерцелуларним микроорганизмима растућег значаја: трансмисија, однос патоген-домаћин, молекуларна епидемиологија и клинички значај (RS-145002)
DOI: 10.1007/s10493-008-9166-6
ISSN: 0168-8162
PubMed: 18551370
WoS: 000258899700006
Scopus: 2-s2.0-51349166142
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Milutinović, Marija AU - Masuzawa, Toshiyuki AU - Tomanović, Snežana AU - Radulović, Željko AU - Fukui, Takashi AU - Okamoto, Yoshihiro PY - 2008 UR - http://rimi.imi.bg.ac.rs/handle/123456789/195 AB - To evaluate the prevalence rate of tick-borne bacterial pathogens, unfed adult Ixodes ricinus ticks were collected from vegetation in 2001, 2003, and 2004 at 18 localities throughout Serbia. A total of 287 ticks were examined by PCR technique for the presence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and Francisella tularensis. The highest prevalence rate was that for B. burgdorferi sensu lato (42.5%), followed by A. phagocytophilum (13.9%) and F. tularensis (3.8%). The presence of five B. burgdorferi sensu lato genospecies, namely, B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. lusitaniae, and B. valaisiana was identified by restriction fragment length polymorphism (RFLP) analysis. The most frequent B. burgdorferi sensu lato genospecies was B. lusitaniae, followed by B. burgdorferi sensu stricto. Co-infection by B. burgdorferi sensu stricto and B. lusitaniae was frequently observed. Co-infection by B. burgdorferi sensu lato and A. phagocytophilum and co-infection by B. burgdorferi sensu lato and F. tularensis appeared in 24 ticks. Sequencing of p44/msp2 paralogs of Serbian A. phagocytophilum showed that they were unique and distinct from those of A. phagocytophilum in US and UK. This is the first report of B. garinii, B. lusitaniae, B. valaisiana, as well as A. phagocytophilum and F. tularensis infected ticks in Serbia. These findings indicate a public health threat in Serbia of tick-borne diseases caused by B. burgdorferi sensu lato, A. phagocytophilum and F. tularensis. PB - Springer, Dordrecht T2 - Experimental & Applied Acarology T1 - Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Francisella tularensis and their co-infections in host-seeking Ixodes ricinus ticks collected in Serbia EP - 183 IS - 3-4 SP - 171 VL - 45 DO - 10.1007/s10493-008-9166-6 ER -
@article{ author = "Milutinović, Marija and Masuzawa, Toshiyuki and Tomanović, Snežana and Radulović, Željko and Fukui, Takashi and Okamoto, Yoshihiro", year = "2008", abstract = "To evaluate the prevalence rate of tick-borne bacterial pathogens, unfed adult Ixodes ricinus ticks were collected from vegetation in 2001, 2003, and 2004 at 18 localities throughout Serbia. A total of 287 ticks were examined by PCR technique for the presence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and Francisella tularensis. The highest prevalence rate was that for B. burgdorferi sensu lato (42.5%), followed by A. phagocytophilum (13.9%) and F. tularensis (3.8%). The presence of five B. burgdorferi sensu lato genospecies, namely, B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. lusitaniae, and B. valaisiana was identified by restriction fragment length polymorphism (RFLP) analysis. The most frequent B. burgdorferi sensu lato genospecies was B. lusitaniae, followed by B. burgdorferi sensu stricto. Co-infection by B. burgdorferi sensu stricto and B. lusitaniae was frequently observed. Co-infection by B. burgdorferi sensu lato and A. phagocytophilum and co-infection by B. burgdorferi sensu lato and F. tularensis appeared in 24 ticks. Sequencing of p44/msp2 paralogs of Serbian A. phagocytophilum showed that they were unique and distinct from those of A. phagocytophilum in US and UK. This is the first report of B. garinii, B. lusitaniae, B. valaisiana, as well as A. phagocytophilum and F. tularensis infected ticks in Serbia. These findings indicate a public health threat in Serbia of tick-borne diseases caused by B. burgdorferi sensu lato, A. phagocytophilum and F. tularensis.", publisher = "Springer, Dordrecht", journal = "Experimental & Applied Acarology", title = "Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Francisella tularensis and their co-infections in host-seeking Ixodes ricinus ticks collected in Serbia", pages = "183-171", number = "3-4", volume = "45", doi = "10.1007/s10493-008-9166-6" }
Milutinović, M., Masuzawa, T., Tomanović, S., Radulović, Ž., Fukui, T.,& Okamoto, Y.. (2008). Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Francisella tularensis and their co-infections in host-seeking Ixodes ricinus ticks collected in Serbia. in Experimental & Applied Acarology Springer, Dordrecht., 45(3-4), 171-183. https://doi.org/10.1007/s10493-008-9166-6
Milutinović M, Masuzawa T, Tomanović S, Radulović Ž, Fukui T, Okamoto Y. Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Francisella tularensis and their co-infections in host-seeking Ixodes ricinus ticks collected in Serbia. in Experimental & Applied Acarology. 2008;45(3-4):171-183. doi:10.1007/s10493-008-9166-6 .
Milutinović, Marija, Masuzawa, Toshiyuki, Tomanović, Snežana, Radulović, Željko, Fukui, Takashi, Okamoto, Yoshihiro, "Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Francisella tularensis and their co-infections in host-seeking Ixodes ricinus ticks collected in Serbia" in Experimental & Applied Acarology, 45, no. 3-4 (2008):171-183, https://doi.org/10.1007/s10493-008-9166-6 . .