Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes
Само за регистроване кориснике
2008
Аутори
Nikolić, MilanRistić-Medić, Danijela
Stanić, Dragana
Poštić, Marija M.
Arsić, Aleksandra
Niketić, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background Blood cholesterol levels are affected by diet and in particular by the type of fat intake. We originally showed that a significant but variable amount of cholesterol is firmly bound to haemoglobin (Hb) yielding the Hb-lipid adduct (Hb-Ch) in erythrocytes isolated from normo-lipidemic males. Aim of the study To establish whether dietary lipids affect the level of Hb-Ch in human erythrocytes. Methods Seventy-four healthy free-living adults were separated according to their serum cholesterol levels into two groups: normo-cholesterolemic (LDL cholesterol lt 3.4 mmol/l and total cholesterol lt 5.2 mmol/l) (NC) and hyper-cholesterolemic (LDL cholesterol gt = 3.4 mmol/l) (HC). Habitual dietary information was used to classify subjects in both study groups into sub-groups of low-fat ( lt = 30% total energy as fat) and high-fat consumers ( gt 30% total energy as fat). The NC low-fat consumers were placed on a high-lipid (high-fat and high-cholesterol) diet whereas the HC subjec...ts with high-fat intake were assigned to a low-lipid (low-fat and low-cholesterol) diet. Both types of dietary intervention were allowed to continue for 6 weeks. The main variable under scrutiny was the Hb-Ch concentration. Results In both study groups low-fat intake subjects had low levels of Hb-Ch (approx. 0.35 mmol/l RBC) compared with high-fat intake subjects (approx. 0.60 mmol/l RBC), and serum cholesterol was not correlated with Hb-Ch. The two dietary interventions produced substantial changes in the Hb-Ch level that paralleled variation in the serum cholesterol concentration. A high-lipid diet (35% fat, 15% saturated; 580 mg cholesterol) increased Hb-Ch (by approximately 47%, P lt 0.001) in subjects with low Hb-Ch at onset, whereas a low-lipid diet (28% fat, 9% saturated; 280 mg cholesterol) decreased Hb-Ch (by approximately 40%, P lt 0.001) in subjects with high Hb-Ch at onset. Conclusion High consumption of dietary lipids, including saturated fat and cholesterol, has an important influence on the level of Hb-Ch in human erythrocytes.
Кључне речи:
dietary lipids / cholesterol / saturated fat / haemoglobin / human erythrocytesИзвор:
European Journal of Nutrition, 2008, 47, 3, 123-130Издавач:
- Springer Heidelberg, Heidelberg
Финансирање / пројекти:
- Хемијске и биохемијске консеквенце метал-лиганд интеракција, II. део (RS-142017)
- Развој нових терапијских поступака у превенцији и лечењу болести јетре: Улога и механизам деловања полинезасићених масних киселина (RS-145071)
DOI: 10.1007/s00394-008-0705-z
ISSN: 1436-6207
PubMed: 18414769
WoS: 000255875600002
Scopus: 2-s2.0-43949085354
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Nikolić, Milan AU - Ristić-Medić, Danijela AU - Stanić, Dragana AU - Poštić, Marija M. AU - Arsić, Aleksandra AU - Niketić, Vesna PY - 2008 UR - http://rimi.imi.bg.ac.rs/handle/123456789/189 AB - Background Blood cholesterol levels are affected by diet and in particular by the type of fat intake. We originally showed that a significant but variable amount of cholesterol is firmly bound to haemoglobin (Hb) yielding the Hb-lipid adduct (Hb-Ch) in erythrocytes isolated from normo-lipidemic males. Aim of the study To establish whether dietary lipids affect the level of Hb-Ch in human erythrocytes. Methods Seventy-four healthy free-living adults were separated according to their serum cholesterol levels into two groups: normo-cholesterolemic (LDL cholesterol lt 3.4 mmol/l and total cholesterol lt 5.2 mmol/l) (NC) and hyper-cholesterolemic (LDL cholesterol gt = 3.4 mmol/l) (HC). Habitual dietary information was used to classify subjects in both study groups into sub-groups of low-fat ( lt = 30% total energy as fat) and high-fat consumers ( gt 30% total energy as fat). The NC low-fat consumers were placed on a high-lipid (high-fat and high-cholesterol) diet whereas the HC subjects with high-fat intake were assigned to a low-lipid (low-fat and low-cholesterol) diet. Both types of dietary intervention were allowed to continue for 6 weeks. The main variable under scrutiny was the Hb-Ch concentration. Results In both study groups low-fat intake subjects had low levels of Hb-Ch (approx. 0.35 mmol/l RBC) compared with high-fat intake subjects (approx. 0.60 mmol/l RBC), and serum cholesterol was not correlated with Hb-Ch. The two dietary interventions produced substantial changes in the Hb-Ch level that paralleled variation in the serum cholesterol concentration. A high-lipid diet (35% fat, 15% saturated; 580 mg cholesterol) increased Hb-Ch (by approximately 47%, P lt 0.001) in subjects with low Hb-Ch at onset, whereas a low-lipid diet (28% fat, 9% saturated; 280 mg cholesterol) decreased Hb-Ch (by approximately 40%, P lt 0.001) in subjects with high Hb-Ch at onset. Conclusion High consumption of dietary lipids, including saturated fat and cholesterol, has an important influence on the level of Hb-Ch in human erythrocytes. PB - Springer Heidelberg, Heidelberg T2 - European Journal of Nutrition T1 - Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes EP - 130 IS - 3 SP - 123 VL - 47 DO - 10.1007/s00394-008-0705-z ER -
@article{ author = "Nikolić, Milan and Ristić-Medić, Danijela and Stanić, Dragana and Poštić, Marija M. and Arsić, Aleksandra and Niketić, Vesna", year = "2008", abstract = "Background Blood cholesterol levels are affected by diet and in particular by the type of fat intake. We originally showed that a significant but variable amount of cholesterol is firmly bound to haemoglobin (Hb) yielding the Hb-lipid adduct (Hb-Ch) in erythrocytes isolated from normo-lipidemic males. Aim of the study To establish whether dietary lipids affect the level of Hb-Ch in human erythrocytes. Methods Seventy-four healthy free-living adults were separated according to their serum cholesterol levels into two groups: normo-cholesterolemic (LDL cholesterol lt 3.4 mmol/l and total cholesterol lt 5.2 mmol/l) (NC) and hyper-cholesterolemic (LDL cholesterol gt = 3.4 mmol/l) (HC). Habitual dietary information was used to classify subjects in both study groups into sub-groups of low-fat ( lt = 30% total energy as fat) and high-fat consumers ( gt 30% total energy as fat). The NC low-fat consumers were placed on a high-lipid (high-fat and high-cholesterol) diet whereas the HC subjects with high-fat intake were assigned to a low-lipid (low-fat and low-cholesterol) diet. Both types of dietary intervention were allowed to continue for 6 weeks. The main variable under scrutiny was the Hb-Ch concentration. Results In both study groups low-fat intake subjects had low levels of Hb-Ch (approx. 0.35 mmol/l RBC) compared with high-fat intake subjects (approx. 0.60 mmol/l RBC), and serum cholesterol was not correlated with Hb-Ch. The two dietary interventions produced substantial changes in the Hb-Ch level that paralleled variation in the serum cholesterol concentration. A high-lipid diet (35% fat, 15% saturated; 580 mg cholesterol) increased Hb-Ch (by approximately 47%, P lt 0.001) in subjects with low Hb-Ch at onset, whereas a low-lipid diet (28% fat, 9% saturated; 280 mg cholesterol) decreased Hb-Ch (by approximately 40%, P lt 0.001) in subjects with high Hb-Ch at onset. Conclusion High consumption of dietary lipids, including saturated fat and cholesterol, has an important influence on the level of Hb-Ch in human erythrocytes.", publisher = "Springer Heidelberg, Heidelberg", journal = "European Journal of Nutrition", title = "Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes", pages = "130-123", number = "3", volume = "47", doi = "10.1007/s00394-008-0705-z" }
Nikolić, M., Ristić-Medić, D., Stanić, D., Poštić, M. M., Arsić, A.,& Niketić, V.. (2008). Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes. in European Journal of Nutrition Springer Heidelberg, Heidelberg., 47(3), 123-130. https://doi.org/10.1007/s00394-008-0705-z
Nikolić M, Ristić-Medić D, Stanić D, Poštić MM, Arsić A, Niketić V. Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes. in European Journal of Nutrition. 2008;47(3):123-130. doi:10.1007/s00394-008-0705-z .
Nikolić, Milan, Ristić-Medić, Danijela, Stanić, Dragana, Poštić, Marija M., Arsić, Aleksandra, Niketić, Vesna, "Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes" in European Journal of Nutrition, 47, no. 3 (2008):123-130, https://doi.org/10.1007/s00394-008-0705-z . .