BMAL1 Regulates Glucokinase Expression Through E-Box Elements In Vitro
Само за регистроване кориснике
2023
Аутори
Llanos, PaulaOrdenes, Patricio
Rhoads, David B.
Santibanez, Juan F.
García-Robles, María
Millán, Carola
Поглавље у монографији (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The organization of a circadian system includes an endogenous pacemaker system, input pathways for environmental synchronizing (entraining) stimuli, and output pathways through which the clock regulates physiological and behavioral processes, for example, the glucose-sensing mechanism in the liver. The liver is the central regulator of metabolism and one of our peripherals clocks. In mammals, central to this pacemaker are the transcription factors Circadian Locomotor Output Cycles Kaput (CLOCK) and BMAL1 (Brain and Muscle ARNT-Like 1). BMAL1 dimerizes with CLOCK, and this heterodimer then binds to the E-box promoter elements (CACGTG) present in clock and clock-controlled genes (CCGs). However, we are just beginning to understand how output pathways and regulatory mechanisms of CCGs are involved in rhythmic physiological processes. Glucokinase (GCK) is a fundamental enzyme in glucose homeostasis, catalyzing the high Km phosphorylation of glucose and allowing its storage. Moreover, gck i...s a dependent circadian gene. This study aims to determine the contribution of clock genes to hepatic gck expression and to define the specific role of E-box sequences on the circadian regulation of hepatic gck. Results showed that gck expression follows a circadian rhythm in rat hepatocytes in vitro. Accordingly, bmal1 expression induces the glucokinase circadian rhythmic expression in hepatocytes and the analysis of human and rat gck promoters, indicating the presence of E-box regions. Moreover, the basal activity of gck promoter was increased by clock/bmal1 co-transfection but inhibited by Period1/Period2 (per1/per2) co-transfection. Thus, the data suggest that the clock proteins tightly regulate the transcriptional activity of the gck promoter.
Кључне речи:
Hepatocytes / Glucokinase / BMAL1 / E-boxИзвор:
Advances in Molecular Pathology, 2023, 1408, 235-249Издавач:
- Springer Nature Switzerland
Финансирање / пројекти:
- Agencia Nacional de Investigacion y Desarrollo-ANID, Chile: Fondecyt inicio N 11121518
- Agencia Nacional de Investigacion y Desarrollo-ANID, Chile: Fondecyt Regular N 1221508
- The grant ICM-ANID P09-022-F from the CENTRO INTERDISCIPLINARIO DE NEUROCIENCIAS DE VALPARAÍSO
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200015 (Универзитет у Београду, Институт за медицинска истраживања) (RS-MESTD-inst-2020-200015)
DOI: 10.1007/978-3-031-26163-3_13
ISBN: 978-3-031-26162-6 (Print)
PubMed: 37093431
[ Google Scholar ]Институција/група
Institut za medicinska istraživanjaTY - CHAP AU - Llanos, Paula AU - Ordenes, Patricio AU - Rhoads, David B. AU - Santibanez, Juan F. AU - García-Robles, María AU - Millán, Carola PY - 2023 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1395 AB - The organization of a circadian system includes an endogenous pacemaker system, input pathways for environmental synchronizing (entraining) stimuli, and output pathways through which the clock regulates physiological and behavioral processes, for example, the glucose-sensing mechanism in the liver. The liver is the central regulator of metabolism and one of our peripherals clocks. In mammals, central to this pacemaker are the transcription factors Circadian Locomotor Output Cycles Kaput (CLOCK) and BMAL1 (Brain and Muscle ARNT-Like 1). BMAL1 dimerizes with CLOCK, and this heterodimer then binds to the E-box promoter elements (CACGTG) present in clock and clock-controlled genes (CCGs). However, we are just beginning to understand how output pathways and regulatory mechanisms of CCGs are involved in rhythmic physiological processes. Glucokinase (GCK) is a fundamental enzyme in glucose homeostasis, catalyzing the high Km phosphorylation of glucose and allowing its storage. Moreover, gck is a dependent circadian gene. This study aims to determine the contribution of clock genes to hepatic gck expression and to define the specific role of E-box sequences on the circadian regulation of hepatic gck. Results showed that gck expression follows a circadian rhythm in rat hepatocytes in vitro. Accordingly, bmal1 expression induces the glucokinase circadian rhythmic expression in hepatocytes and the analysis of human and rat gck promoters, indicating the presence of E-box regions. Moreover, the basal activity of gck promoter was increased by clock/bmal1 co-transfection but inhibited by Period1/Period2 (per1/per2) co-transfection. Thus, the data suggest that the clock proteins tightly regulate the transcriptional activity of the gck promoter. PB - Springer Nature Switzerland T2 - Advances in Molecular Pathology T2 - Advances in Molecular Pathology T1 - BMAL1 Regulates Glucokinase Expression Through E-Box Elements In Vitro EP - 249 SP - 235 VL - 1408 DO - 10.1007/978-3-031-26163-3_13 ER -
@inbook{ author = "Llanos, Paula and Ordenes, Patricio and Rhoads, David B. and Santibanez, Juan F. and García-Robles, María and Millán, Carola", year = "2023", abstract = "The organization of a circadian system includes an endogenous pacemaker system, input pathways for environmental synchronizing (entraining) stimuli, and output pathways through which the clock regulates physiological and behavioral processes, for example, the glucose-sensing mechanism in the liver. The liver is the central regulator of metabolism and one of our peripherals clocks. In mammals, central to this pacemaker are the transcription factors Circadian Locomotor Output Cycles Kaput (CLOCK) and BMAL1 (Brain and Muscle ARNT-Like 1). BMAL1 dimerizes with CLOCK, and this heterodimer then binds to the E-box promoter elements (CACGTG) present in clock and clock-controlled genes (CCGs). However, we are just beginning to understand how output pathways and regulatory mechanisms of CCGs are involved in rhythmic physiological processes. Glucokinase (GCK) is a fundamental enzyme in glucose homeostasis, catalyzing the high Km phosphorylation of glucose and allowing its storage. Moreover, gck is a dependent circadian gene. This study aims to determine the contribution of clock genes to hepatic gck expression and to define the specific role of E-box sequences on the circadian regulation of hepatic gck. Results showed that gck expression follows a circadian rhythm in rat hepatocytes in vitro. Accordingly, bmal1 expression induces the glucokinase circadian rhythmic expression in hepatocytes and the analysis of human and rat gck promoters, indicating the presence of E-box regions. Moreover, the basal activity of gck promoter was increased by clock/bmal1 co-transfection but inhibited by Period1/Period2 (per1/per2) co-transfection. Thus, the data suggest that the clock proteins tightly regulate the transcriptional activity of the gck promoter.", publisher = "Springer Nature Switzerland", journal = "Advances in Molecular Pathology, Advances in Molecular Pathology", booktitle = "BMAL1 Regulates Glucokinase Expression Through E-Box Elements In Vitro", pages = "249-235", volume = "1408", doi = "10.1007/978-3-031-26163-3_13" }
Llanos, P., Ordenes, P., Rhoads, D. B., Santibanez, J. F., García-Robles, M.,& Millán, C.. (2023). BMAL1 Regulates Glucokinase Expression Through E-Box Elements In Vitro. in Advances in Molecular Pathology Springer Nature Switzerland., 1408, 235-249. https://doi.org/10.1007/978-3-031-26163-3_13
Llanos P, Ordenes P, Rhoads DB, Santibanez JF, García-Robles M, Millán C. BMAL1 Regulates Glucokinase Expression Through E-Box Elements In Vitro. in Advances in Molecular Pathology. 2023;1408:235-249. doi:10.1007/978-3-031-26163-3_13 .
Llanos, Paula, Ordenes, Patricio, Rhoads, David B., Santibanez, Juan F., García-Robles, María, Millán, Carola, "BMAL1 Regulates Glucokinase Expression Through E-Box Elements In Vitro" in Advances in Molecular Pathology, 1408 (2023):235-249, https://doi.org/10.1007/978-3-031-26163-3_13 . .