Human amniotic membrane inhibits migration and invasion of muscle-invasive bladder cancer urothelial cells by downregulating the FAK/PI3K/Akt/mTOR signalling pathway
Аутори
Janev, AleksandarŽeleznik Ramuta, Taja
Dragin Jerman, Urška
Obradović, Hristina
Kamenšek, Urška
Čemažar, Maja
Erdani Kreft, Mateja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Bladder cancer is the 10th most commonly diagnosed cancer with the highest lifetime treatment costs. The human amniotic membrane (hAM) is the innermost foetal membrane that possesses a wide range of biological properties, including anti-inflammatory, antimicrobial and anticancer properties. Despite the growing number of studies, the mechanisms associated with the anticancer effects of human amniotic membrane (hAM) are poorly understood. Here, we reported that hAM preparations (homogenate and extract) inhibited the expression of the epithelial–mesenchymal transition markers N-cadherin and MMP-2 in bladder cancer urothelial cells in a dose-dependent manner, while increasing the secretion of TIMP-2. Moreover, hAM homogenate exerted its antimigratory effect by downregulating the expression of FAK and proteins involved in actin cytoskeleton reorganisation, such as cortactin and small RhoGTPases. In muscle-invasive cancer urothelial cells, hAM homogenate downregulated the PI3K/Akt/mTOR signa...lling pathway, the key cascade involved in promoting bladder cancer. By using normal, non-invasive papilloma and muscle-invasive cancer urothelial models, new perspectives on the anticancer effects of hAM have emerged. The results identify new sites for therapeutic intervention and are prompt encouragement for ongoing anticancer drug development studies.
Извор:
Scientific Reports, 2023, 13, 1, 19227-Издавач:
- Springer Nature
Финансирање / пројекти:
- Slovenian Research Agency (Young-Researcher funding, project J7-2594, J2-3043 and research core funding No. P3-0108, P3-0003) and MRIC UL IP-0510 Infrastructure program
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Janev, Aleksandar AU - Železnik Ramuta, Taja AU - Dragin Jerman, Urška AU - Obradović, Hristina AU - Kamenšek, Urška AU - Čemažar, Maja AU - Erdani Kreft, Mateja PY - 2023 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1381 AB - Bladder cancer is the 10th most commonly diagnosed cancer with the highest lifetime treatment costs. The human amniotic membrane (hAM) is the innermost foetal membrane that possesses a wide range of biological properties, including anti-inflammatory, antimicrobial and anticancer properties. Despite the growing number of studies, the mechanisms associated with the anticancer effects of human amniotic membrane (hAM) are poorly understood. Here, we reported that hAM preparations (homogenate and extract) inhibited the expression of the epithelial–mesenchymal transition markers N-cadherin and MMP-2 in bladder cancer urothelial cells in a dose-dependent manner, while increasing the secretion of TIMP-2. Moreover, hAM homogenate exerted its antimigratory effect by downregulating the expression of FAK and proteins involved in actin cytoskeleton reorganisation, such as cortactin and small RhoGTPases. In muscle-invasive cancer urothelial cells, hAM homogenate downregulated the PI3K/Akt/mTOR signalling pathway, the key cascade involved in promoting bladder cancer. By using normal, non-invasive papilloma and muscle-invasive cancer urothelial models, new perspectives on the anticancer effects of hAM have emerged. The results identify new sites for therapeutic intervention and are prompt encouragement for ongoing anticancer drug development studies. PB - Springer Nature T2 - Scientific Reports T2 - Scientific Reports T1 - Human amniotic membrane inhibits migration and invasion of muscle-invasive bladder cancer urothelial cells by downregulating the FAK/PI3K/Akt/mTOR signalling pathway IS - 1 SP - 19227 VL - 13 DO - 10.1038/s41598-023-46091-2 ER -
@article{ author = "Janev, Aleksandar and Železnik Ramuta, Taja and Dragin Jerman, Urška and Obradović, Hristina and Kamenšek, Urška and Čemažar, Maja and Erdani Kreft, Mateja", year = "2023", abstract = "Bladder cancer is the 10th most commonly diagnosed cancer with the highest lifetime treatment costs. The human amniotic membrane (hAM) is the innermost foetal membrane that possesses a wide range of biological properties, including anti-inflammatory, antimicrobial and anticancer properties. Despite the growing number of studies, the mechanisms associated with the anticancer effects of human amniotic membrane (hAM) are poorly understood. Here, we reported that hAM preparations (homogenate and extract) inhibited the expression of the epithelial–mesenchymal transition markers N-cadherin and MMP-2 in bladder cancer urothelial cells in a dose-dependent manner, while increasing the secretion of TIMP-2. Moreover, hAM homogenate exerted its antimigratory effect by downregulating the expression of FAK and proteins involved in actin cytoskeleton reorganisation, such as cortactin and small RhoGTPases. In muscle-invasive cancer urothelial cells, hAM homogenate downregulated the PI3K/Akt/mTOR signalling pathway, the key cascade involved in promoting bladder cancer. By using normal, non-invasive papilloma and muscle-invasive cancer urothelial models, new perspectives on the anticancer effects of hAM have emerged. The results identify new sites for therapeutic intervention and are prompt encouragement for ongoing anticancer drug development studies.", publisher = "Springer Nature", journal = "Scientific Reports, Scientific Reports", title = "Human amniotic membrane inhibits migration and invasion of muscle-invasive bladder cancer urothelial cells by downregulating the FAK/PI3K/Akt/mTOR signalling pathway", number = "1", pages = "19227", volume = "13", doi = "10.1038/s41598-023-46091-2" }
Janev, A., Železnik Ramuta, T., Dragin Jerman, U., Obradović, H., Kamenšek, U., Čemažar, M.,& Erdani Kreft, M.. (2023). Human amniotic membrane inhibits migration and invasion of muscle-invasive bladder cancer urothelial cells by downregulating the FAK/PI3K/Akt/mTOR signalling pathway. in Scientific Reports Springer Nature., 13(1), 19227. https://doi.org/10.1038/s41598-023-46091-2
Janev A, Železnik Ramuta T, Dragin Jerman U, Obradović H, Kamenšek U, Čemažar M, Erdani Kreft M. Human amniotic membrane inhibits migration and invasion of muscle-invasive bladder cancer urothelial cells by downregulating the FAK/PI3K/Akt/mTOR signalling pathway. in Scientific Reports. 2023;13(1):19227. doi:10.1038/s41598-023-46091-2 .
Janev, Aleksandar, Železnik Ramuta, Taja, Dragin Jerman, Urška, Obradović, Hristina, Kamenšek, Urška, Čemažar, Maja, Erdani Kreft, Mateja, "Human amniotic membrane inhibits migration and invasion of muscle-invasive bladder cancer urothelial cells by downregulating the FAK/PI3K/Akt/mTOR signalling pathway" in Scientific Reports, 13, no. 1 (2023):19227, https://doi.org/10.1038/s41598-023-46091-2 . .