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Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract
dc.creator | Jovanović, Aleksandra A. | |
dc.creator | Vajić, Una Jovana | |
dc.creator | Mijin, Dušan Z. | |
dc.creator | Zdunić, Gordana M. | |
dc.creator | Šavikin, Katarina P. | |
dc.creator | Branković, Suzana | |
dc.creator | Kitić, Dušanka | |
dc.creator | Bugarski, Branko | |
dc.date.accessioned | 2023-08-11T11:40:50Z | |
dc.date.available | 2023-08-11T11:40:50Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 2214-7861 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/1332 | |
dc.description.abstract | In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products. | |
dc.language | en | |
dc.publisher | Elsevier | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200287/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200003/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200113/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of Applied Research on Medicinal and Aromatic Plants | |
dc.source | Journal of Applied Research on Medicinal and Aromatic Plants | |
dc.subject | Antimicrobial activity | |
dc.subject | Microwave reactor | |
dc.subject | Polyphenols | |
dc.subject | Spasmolytic activity | |
dc.subject | Wild thyme by-product | |
dc.title | Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract | |
dc.type | article | en |
dc.rights.license | ARR | |
dc.citation.spage | 100417 | |
dc.citation.volume | 31 | |
dc.identifier.doi | 10.1016/j.jarmap.2022.100417 | |
dc.type.version | publishedVersion |