Differences in the relationship of specificity to titre and functional affinity between circulating Ga- and pan-reactive IgM rheumatoid factors in rheumatoid arthritis
Само за регистроване кориснике
2004
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objective. To determine if there are the differences in titre and functional affinity for immunoglobulin (Ig) G subclasses and glycoforms between the Ga- and pan-specific IgM rheumatoid factors (RFs) present in the sera of patients with rheumatoid arthritis (RA), and to determine whether these two broad specificities have different functional roles in RA.Methods. We used direct ELISA and modified ELISA to study the binding of IgM RF in the sera of 32 patients with RA with a range of RF titres to a panel of 14 IgG paraproteins of all four subclasses, some allotypes and different glycosylation patterns.Results. Pan-specific RFs were mostly found in RA sera with high RF titres, and these RFs generally had higher avidity. A trend towards higher avidity of RFs with higher titre was observed for pan-specific, but not for Ga-specific RFs. With increasing titre, pan-specific RFs tended to react strongly with fucosylated and bisected variants of hypogalactosylated IgG3 of G3m(b1) allotype and h...ypergalactosylated IgG4 of 4a allotype.Conclusion. Among high-titred pan-specific IgM RFs, there is a subpopulation responsible for strong anti-IgG activity in RA. The possible mechanisms of production of pan- and Ga-specific RFs are discussed.
Кључне речи:
Pan and Ga RFs / Avidity / Titre / IgG glycoforms / Rheumatoid arthritisИзвор:
Rheumatology, 2004, 43, 9, 1190-1193Издавач:
- Oxford University Press
Финансирање / пројекти:
- This work was supported by a Basic Science Grant from the Ministry of Science and Technology of Serbia.
- The authors are grateful to Professor Royston Jefferis, University of Birmingham, UK, for providing a set of IgG standards of known %G0.
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Milošević-Jovčić, N AU - Ćirić, Dragana AU - Hajduković-Dragojlović, L. AU - Mirčetić, V. PY - 2004 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1326 AB - Objective. To determine if there are the differences in titre and functional affinity for immunoglobulin (Ig) G subclasses and glycoforms between the Ga- and pan-specific IgM rheumatoid factors (RFs) present in the sera of patients with rheumatoid arthritis (RA), and to determine whether these two broad specificities have different functional roles in RA.Methods. We used direct ELISA and modified ELISA to study the binding of IgM RF in the sera of 32 patients with RA with a range of RF titres to a panel of 14 IgG paraproteins of all four subclasses, some allotypes and different glycosylation patterns.Results. Pan-specific RFs were mostly found in RA sera with high RF titres, and these RFs generally had higher avidity. A trend towards higher avidity of RFs with higher titre was observed for pan-specific, but not for Ga-specific RFs. With increasing titre, pan-specific RFs tended to react strongly with fucosylated and bisected variants of hypogalactosylated IgG3 of G3m(b1) allotype and hypergalactosylated IgG4 of 4a allotype.Conclusion. Among high-titred pan-specific IgM RFs, there is a subpopulation responsible for strong anti-IgG activity in RA. The possible mechanisms of production of pan- and Ga-specific RFs are discussed. PB - Oxford University Press T2 - Rheumatology T1 - Differences in the relationship of specificity to titre and functional affinity between circulating Ga- and pan-reactive IgM rheumatoid factors in rheumatoid arthritis EP - 1193 IS - 9 SP - 1190 VL - 43 DO - 10.1093/rheumatology/keh287 ER -
@article{ author = "Milošević-Jovčić, N and Ćirić, Dragana and Hajduković-Dragojlović, L. and Mirčetić, V.", year = "2004", abstract = "Objective. To determine if there are the differences in titre and functional affinity for immunoglobulin (Ig) G subclasses and glycoforms between the Ga- and pan-specific IgM rheumatoid factors (RFs) present in the sera of patients with rheumatoid arthritis (RA), and to determine whether these two broad specificities have different functional roles in RA.Methods. We used direct ELISA and modified ELISA to study the binding of IgM RF in the sera of 32 patients with RA with a range of RF titres to a panel of 14 IgG paraproteins of all four subclasses, some allotypes and different glycosylation patterns.Results. Pan-specific RFs were mostly found in RA sera with high RF titres, and these RFs generally had higher avidity. A trend towards higher avidity of RFs with higher titre was observed for pan-specific, but not for Ga-specific RFs. With increasing titre, pan-specific RFs tended to react strongly with fucosylated and bisected variants of hypogalactosylated IgG3 of G3m(b1) allotype and hypergalactosylated IgG4 of 4a allotype.Conclusion. Among high-titred pan-specific IgM RFs, there is a subpopulation responsible for strong anti-IgG activity in RA. The possible mechanisms of production of pan- and Ga-specific RFs are discussed.", publisher = "Oxford University Press", journal = "Rheumatology", title = "Differences in the relationship of specificity to titre and functional affinity between circulating Ga- and pan-reactive IgM rheumatoid factors in rheumatoid arthritis", pages = "1193-1190", number = "9", volume = "43", doi = "10.1093/rheumatology/keh287" }
Milošević-Jovčić, N., Ćirić, D., Hajduković-Dragojlović, L.,& Mirčetić, V.. (2004). Differences in the relationship of specificity to titre and functional affinity between circulating Ga- and pan-reactive IgM rheumatoid factors in rheumatoid arthritis. in Rheumatology Oxford University Press., 43(9), 1190-1193. https://doi.org/10.1093/rheumatology/keh287
Milošević-Jovčić N, Ćirić D, Hajduković-Dragojlović L, Mirčetić V. Differences in the relationship of specificity to titre and functional affinity between circulating Ga- and pan-reactive IgM rheumatoid factors in rheumatoid arthritis. in Rheumatology. 2004;43(9):1190-1193. doi:10.1093/rheumatology/keh287 .
Milošević-Jovčić, N, Ćirić, Dragana, Hajduković-Dragojlović, L., Mirčetić, V., "Differences in the relationship of specificity to titre and functional affinity between circulating Ga- and pan-reactive IgM rheumatoid factors in rheumatoid arthritis" in Rheumatology, 43, no. 9 (2004):1190-1193, https://doi.org/10.1093/rheumatology/keh287 . .