Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier
Аутори
Janković, MarkoĆirković, Valentina
Stamenković, Gorana
Lončar, Ana
Todorović, Marija
Stanojević, Maja
Šiljić, Marina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Here, we report on a serendipitous finding of a chryso-like virus associated with Culex pipiens mosquitos in the course of study aimed to detect and characterize West Nile virus (WNV) circulating in mosquitos in Serbia, Southern Europe. Upon initial detection of unexpected product in a PCR protocol for partial WNV NS5 gene amplification, further confirmation and identification was obtained through additional PCR and Sanger sequencing experiments. Bioinformatic and phylogenetic analysis identified the obtained sequences as Xanthi chryso-like virus (XCLV). The finding is particular for the fact that it associates XCLV with a new potential vector species and documents a novel geographical area of its distribution.
Кључне речи:
Culex pipiens / arthropod-borne / chrysovirus / mosquito / mycovirus / RNA virus / sequencing / SerbiaИзвор:
Tropical Medicine and Infectious Disease, 2023, 8, 4, 225-Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200110 (Универзитет у Београду, Медицински факултет) (RS-MESTD-inst-2020-200110)
- IN-DEPTH - An Evolutionary Insight Into Molecular Diversity of Emerging Pathogens in Serbia Through Phylogenetic Approach (RS-ScienceFundRS-Promis-6059147)
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Janković, Marko AU - Ćirković, Valentina AU - Stamenković, Gorana AU - Lončar, Ana AU - Todorović, Marija AU - Stanojević, Maja AU - Šiljić, Marina PY - 2023 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1293 AB - Here, we report on a serendipitous finding of a chryso-like virus associated with Culex pipiens mosquitos in the course of study aimed to detect and characterize West Nile virus (WNV) circulating in mosquitos in Serbia, Southern Europe. Upon initial detection of unexpected product in a PCR protocol for partial WNV NS5 gene amplification, further confirmation and identification was obtained through additional PCR and Sanger sequencing experiments. Bioinformatic and phylogenetic analysis identified the obtained sequences as Xanthi chryso-like virus (XCLV). The finding is particular for the fact that it associates XCLV with a new potential vector species and documents a novel geographical area of its distribution. PB - MDPI T2 - Tropical Medicine and Infectious Disease T1 - Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier IS - 4 SP - 225 VL - 8 DO - 10.3390/tropicalmed8040225 ER -
@article{ author = "Janković, Marko and Ćirković, Valentina and Stamenković, Gorana and Lončar, Ana and Todorović, Marija and Stanojević, Maja and Šiljić, Marina", year = "2023", abstract = "Here, we report on a serendipitous finding of a chryso-like virus associated with Culex pipiens mosquitos in the course of study aimed to detect and characterize West Nile virus (WNV) circulating in mosquitos in Serbia, Southern Europe. Upon initial detection of unexpected product in a PCR protocol for partial WNV NS5 gene amplification, further confirmation and identification was obtained through additional PCR and Sanger sequencing experiments. Bioinformatic and phylogenetic analysis identified the obtained sequences as Xanthi chryso-like virus (XCLV). The finding is particular for the fact that it associates XCLV with a new potential vector species and documents a novel geographical area of its distribution.", publisher = "MDPI", journal = "Tropical Medicine and Infectious Disease", title = "Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier", number = "4", pages = "225", volume = "8", doi = "10.3390/tropicalmed8040225" }
Janković, M., Ćirković, V., Stamenković, G., Lončar, A., Todorović, M., Stanojević, M.,& Šiljić, M.. (2023). Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier. in Tropical Medicine and Infectious Disease MDPI., 8(4), 225. https://doi.org/10.3390/tropicalmed8040225
Janković M, Ćirković V, Stamenković G, Lončar A, Todorović M, Stanojević M, Šiljić M. Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier. in Tropical Medicine and Infectious Disease. 2023;8(4):225. doi:10.3390/tropicalmed8040225 .
Janković, Marko, Ćirković, Valentina, Stamenković, Gorana, Lončar, Ana, Todorović, Marija, Stanojević, Maja, Šiljić, Marina, "Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier" in Tropical Medicine and Infectious Disease, 8, no. 4 (2023):225, https://doi.org/10.3390/tropicalmed8040225 . .