TY  - CONF
AU  - Mitrović-Ajtić, Olivera
AU  - Đikić, Dragoslava
AU  - Dragojević, Teodora
AU  - Otašević, Vladimir
AU  - Živković, Emilija
AU  - Vuković, Vojin
AU  - Vukotić, Milica
AU  - Subotički, Tijana
AU  - Diklić, Miloš
AU  - Suvajdžić-Vuković, Nada
AU  - Mitrović, Mirjana
AU  - Mihaljević, Biljana
AU  - Antić, Darko
AU  - Čokić, Vladan
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1420
AB  - Introduction: Patients with hematological malignancies have an increased risk of thrombotic complications, ranging from 3-5% in patients with lymphoma and acute myeloid leukemia (AML). The presented study observed the onset of thrombus formation to predict risk factors for thrombosis in lymphoid and myeloid malignancies. Methods: Coagulation factors, inflammatory signaling pathways and adhesion molecules have been observed in patients with Hodgkin lymphoma (HL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL) and AML. Their mononuclear cells (MNC) trans-endothelial migration through human microvascular endothelial cells (HMEC-1) monolayer is observed by Boyden chamber.
Results: Thrombin was in positive correlation with tumor necrosis factor alpha (TNF-α) in HL, while with P-selectin (p<0.001), tumor growth factor-beta (TGF-β) and factor VIII (p<0.05) in DLBCL and AML. Transendothelial migration of MNC was increased by TNF-α (p<0.001) in DLBCL regardless of previous thrombosis. Regarding coagulation, factor VIII was increased in HL and AML (p<0.05), while tissue factor in non-Hodgkin lymphomas (DLBCL and FL, p<0.05). Tissue factor was in positive correlation with adhesion molecule P-selectin and factor VIII (p<0.05). P-selectin was increased in non-Hodgkin lymphomas (p<0.0001), while TGF-β only in FL (p<0.001). Fibrinolytic activity was decreased in plasma of patients with HL, DLBCL, and FL (p<0.05), but largely in AML (p<0.01) as measured by tissue-type plasminogen activator. Inflammatory NF-κB signaling has been activated in HL and DLBCL, while p38 signaling only in HL. Conclusion: Coagulation factors and inflammation are increased in hematological malignancies along with the interaction of the endothelium and circulating cells that predispose to thrombus formation
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Activation of coagulation factors and prothrombic properties of endothelium in hematological malignancies
EP  - 134
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1420
ER  - 

@conference{
author = "Mitrović-Ajtić, Olivera and Đikić, Dragoslava and Dragojević, Teodora and Otašević, Vladimir and Živković, Emilija and Vuković, Vojin and Vukotić, Milica and Subotički, Tijana and Diklić, Miloš and Suvajdžić-Vuković, Nada and Mitrović, Mirjana and Mihaljević, Biljana and Antić, Darko and Čokić, Vladan",
year = "2023",
abstract = "Introduction: Patients with hematological malignancies have an increased risk of thrombotic complications, ranging from 3-5% in patients with lymphoma and acute myeloid leukemia (AML). The presented study observed the onset of thrombus formation to predict risk factors for thrombosis in lymphoid and myeloid malignancies. Methods: Coagulation factors, inflammatory signaling pathways and adhesion molecules have been observed in patients with Hodgkin lymphoma (HL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL) and AML. Their mononuclear cells (MNC) trans-endothelial migration through human microvascular endothelial cells (HMEC-1) monolayer is observed by Boyden chamber.
Results: Thrombin was in positive correlation with tumor necrosis factor alpha (TNF-α) in HL, while with P-selectin (p<0.001), tumor growth factor-beta (TGF-β) and factor VIII (p<0.05) in DLBCL and AML. Transendothelial migration of MNC was increased by TNF-α (p<0.001) in DLBCL regardless of previous thrombosis. Regarding coagulation, factor VIII was increased in HL and AML (p<0.05), while tissue factor in non-Hodgkin lymphomas (DLBCL and FL, p<0.05). Tissue factor was in positive correlation with adhesion molecule P-selectin and factor VIII (p<0.05). P-selectin was increased in non-Hodgkin lymphomas (p<0.0001), while TGF-β only in FL (p<0.001). Fibrinolytic activity was decreased in plasma of patients with HL, DLBCL, and FL (p<0.05), but largely in AML (p<0.01) as measured by tissue-type plasminogen activator. Inflammatory NF-κB signaling has been activated in HL and DLBCL, while p38 signaling only in HL. Conclusion: Coagulation factors and inflammation are increased in hematological malignancies along with the interaction of the endothelium and circulating cells that predispose to thrombus formation",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Activation of coagulation factors and prothrombic properties of endothelium in hematological malignancies",
pages = "134-134",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1420"
}

Mitrović-Ajtić, O., Đikić, D., Dragojević, T., Otašević, V., Živković, E., Vuković, V., Vukotić, M., Subotički, T., Diklić, M., Suvajdžić-Vuković, N., Mitrović, M., Mihaljević, B., Antić, D.,& Čokić, V.. (2023). Activation of coagulation factors and prothrombic properties of endothelium in hematological malignancies. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 134-134.
https://hdl.handle.net/21.15107/rcub_rimi_1420

Mitrović-Ajtić O, Đikić D, Dragojević T, Otašević V, Živković E, Vuković V, Vukotić M, Subotički T, Diklić M, Suvajdžić-Vuković N, Mitrović M, Mihaljević B, Antić D, Čokić V. Activation of coagulation factors and prothrombic properties of endothelium in hematological malignancies. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:134-134.
https://hdl.handle.net/21.15107/rcub_rimi_1420 .

Mitrović-Ajtić, Olivera, Đikić, Dragoslava, Dragojević, Teodora, Otašević, Vladimir, Živković, Emilija, Vuković, Vojin, Vukotić, Milica, Subotički, Tijana, Diklić, Miloš, Suvajdžić-Vuković, Nada, Mitrović, Mirjana, Mihaljević, Biljana, Antić, Darko, Čokić, Vladan, "Activation of coagulation factors and prothrombic properties of endothelium in hematological malignancies" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):134-134,
https://hdl.handle.net/21.15107/rcub_rimi_1420 .