TY  - JOUR
AU  - Resanović, Ivana
AU  - Gluvić, Zoran
AU  - Zarić, Božidarka
AU  - Sudar-Milovanović, Emina
AU  - Vučić, Vesna M.
AU  - Arsić, Aleksandra
AU  - Nedić, Olgica
AU  - Sunderić, Miloš
AU  - Gligorijević, Nikola
AU  - Milačić, Davorka
AU  - Isenović, Esma R.
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1042
AB  - Objective: Metabolic changes in type 1 diabetes mellitus (TIDM) impair vasodilation, and this leads to tissue hypoxia and microvascular pathology. Hyperbaric oxygen therapy (HBOT) can significantly improve the outcome of ischemic conditions in T1DM patients and reduce vascular complications. The aim of our study was to assess the effects of HBOT on plasma fatty acid (FA) composition, and expression of insulin-like growth factor binding protein 1 (IGFBP-1) in T1DM patients. Methods: Our study included 24 adult T1DM patients diagnosed with peripheral vascular complications. The patients were exposed to 10 sessions of 100% oxygen inhalation at 2.4 atmosphere absolute for 1 hour. Blood samples were collected at admission and after HBOT for measurement of metabolic parameters, FA composition and IGFBP-1. Measurement of plasma FA composition was determined by gas chromatography. Expression of IGFBP-1 in the serum was estimated by Western blot analysis. Results: HBOT decreased blood levels of total cholesterol (p lt 0.05), triglycerides (p lt 0.05) and low-density lipoprotein (p lt 0.05). HBOT increased plasma levels of individual FAs: palmitic acid (p lt 0.05), palmitoleic acid (p lt 0.05), docosapentaenoic acid (p lt 0.05) and docosahexaenoic acid (p lt 0.01), and decreased levels of stearic acid (p lt 0.05), alpha linolenic acid (p lt 0.05) and linoleic acid (p lt 0.01). Expression of IGFBP-1 (p lt 0.01) was increased, whereas the level of insulin (p lt 0.001) was decreased in the serum after HBOT. Conclusions: Our results indicate that HBOT exerts beneficial effects in TIDM patients by improving the lipid profile and altering FA composition.
PB  - Elsevier, Amsterdam
T2  - Canadian Journal of Diabetes
T1  - Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study
EP  - 29
IS  - 1
SP  - 22
VL  - 44
DO  - 10.1016/j.jcjd.2019.04.018
ER  - 

@article{
author = "Resanović, Ivana and Gluvić, Zoran and Zarić, Božidarka and Sudar-Milovanović, Emina and Vučić, Vesna M. and Arsić, Aleksandra and Nedić, Olgica and Sunderić, Miloš and Gligorijević, Nikola and Milačić, Davorka and Isenović, Esma R.",
year = "2020",
abstract = "Objective: Metabolic changes in type 1 diabetes mellitus (TIDM) impair vasodilation, and this leads to tissue hypoxia and microvascular pathology. Hyperbaric oxygen therapy (HBOT) can significantly improve the outcome of ischemic conditions in T1DM patients and reduce vascular complications. The aim of our study was to assess the effects of HBOT on plasma fatty acid (FA) composition, and expression of insulin-like growth factor binding protein 1 (IGFBP-1) in T1DM patients. Methods: Our study included 24 adult T1DM patients diagnosed with peripheral vascular complications. The patients were exposed to 10 sessions of 100% oxygen inhalation at 2.4 atmosphere absolute for 1 hour. Blood samples were collected at admission and after HBOT for measurement of metabolic parameters, FA composition and IGFBP-1. Measurement of plasma FA composition was determined by gas chromatography. Expression of IGFBP-1 in the serum was estimated by Western blot analysis. Results: HBOT decreased blood levels of total cholesterol (p lt 0.05), triglycerides (p lt 0.05) and low-density lipoprotein (p lt 0.05). HBOT increased plasma levels of individual FAs: palmitic acid (p lt 0.05), palmitoleic acid (p lt 0.05), docosapentaenoic acid (p lt 0.05) and docosahexaenoic acid (p lt 0.01), and decreased levels of stearic acid (p lt 0.05), alpha linolenic acid (p lt 0.05) and linoleic acid (p lt 0.01). Expression of IGFBP-1 (p lt 0.01) was increased, whereas the level of insulin (p lt 0.001) was decreased in the serum after HBOT. Conclusions: Our results indicate that HBOT exerts beneficial effects in TIDM patients by improving the lipid profile and altering FA composition.",
publisher = "Elsevier, Amsterdam",
journal = "Canadian Journal of Diabetes",
title = "Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study",
pages = "29-22",
number = "1",
volume = "44",
doi = "10.1016/j.jcjd.2019.04.018"
}

Resanović, I., Gluvić, Z., Zarić, B., Sudar-Milovanović, E., Vučić, V. M., Arsić, A., Nedić, O., Sunderić, M., Gligorijević, N., Milačić, D.,& Isenović, E. R.. (2020). Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study. in Canadian Journal of Diabetes
Elsevier, Amsterdam., 44(1), 22-29.
https://doi.org/10.1016/j.jcjd.2019.04.018

Resanović I, Gluvić Z, Zarić B, Sudar-Milovanović E, Vučić VM, Arsić A, Nedić O, Sunderić M, Gligorijević N, Milačić D, Isenović ER. Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study. in Canadian Journal of Diabetes. 2020;44(1):22-29.
doi:10.1016/j.jcjd.2019.04.018 .

Resanović, Ivana, Gluvić, Zoran, Zarić, Božidarka, Sudar-Milovanović, Emina, Vučić, Vesna M., Arsić, Aleksandra, Nedić, Olgica, Sunderić, Miloš, Gligorijević, Nikola, Milačić, Davorka, Isenović, Esma R., "Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study" in Canadian Journal of Diabetes, 44, no. 1 (2020):22-29,
https://doi.org/10.1016/j.jcjd.2019.04.018 . .

TY  - JOUR
AU  - Nedić, Olgica
AU  - Sunderić, Miloš
AU  - Miljuš, Goran
AU  - Valdevit, Zoran
AU  - Jakovljević, Vladimir
AU  - Glibetić, Marija
AU  - Vučić, Vesna M.
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/759
AB  - Background: Intensive exercise changes physiological need for glucose and several biochemical pathways responsible for its metabolism response. Among them are those which involve insulin, insulin-like growth factor (IGF-1), and IGF-binding proteins (IGFBPs). Different types and degrees of exercise, as well as an athlete's fitness, may induce a range of responses regarding concentrations and time needed for the alteration. The idea of the work was to find out whether and how insulin/IGF axis responds to additional physical activity in the already trained subjects and if so, is the adaptation potentially beneficial from the aspect of metabolic control. Methods: The effect of 4-week intensive training on campus (preparatory training) on the levels of insulin, IGF-1, and IGFBPs during maximal progressive exercise test (MPET) on a treadmill was compared to the results obtained during MPET conducted after a regular training season of a female elite handball team (n = 17, age: 17 +/- 1 years, height: 171 +/- 8 cm, weight: 65 +/- 8 kg, body mass index: 22 +/- 1 kg/m(2) at the beginning of the study; there were no significant changes at the end). Serum samples were obtained from players immediately before the test (basal), at the end of the test after reaching the point of maximal oxygen consumption (VO2max), and after recovery. Results: The concentration of insulin decreased at VO2max, but remained higher in players after preparatory training (12.2 +/- 2.5 mU/L vs. 8.9 +/- 4.4 mU/L, p = 0.049). The level of IGFBP-1 decreased in players at VO2max in either case of training, but it remained much higher in tests performed after the preparatory regime than before (p = 0.029). Concentrations of IGF-1, IGFBP-2, -3, and -4 did not change significantly. Conclusion: The inverse relation between insulin and IGFBP-1 was lost during MPET, as these 2 molecules changed in the same direction. The results obtained suggest less severe stress-induced depression of insulin and IGFBP-1 after preparatory training. But another metabolic mechanism cannot be excluded, and that is potentially impaired insulin sensitivity resulting in higher level of IGFBP-1.
PB  - Shanghai Univ Sport, Shanghai
T2  - Journal of Sport & Health Science
T1  - Preparatory training attenuates drastic response of the insulin-like growth factor binding protein 1 at the point of maximal oxygen consumption in handball players
EP  - 377
IS  - 3
SP  - 372
VL  - 6
DO  - 10.1016/j.jshs.2016.01.014
ER  - 

@article{
author = "Nedić, Olgica and Sunderić, Miloš and Miljuš, Goran and Valdevit, Zoran and Jakovljević, Vladimir and Glibetić, Marija and Vučić, Vesna M.",
year = "2017",
abstract = "Background: Intensive exercise changes physiological need for glucose and several biochemical pathways responsible for its metabolism response. Among them are those which involve insulin, insulin-like growth factor (IGF-1), and IGF-binding proteins (IGFBPs). Different types and degrees of exercise, as well as an athlete's fitness, may induce a range of responses regarding concentrations and time needed for the alteration. The idea of the work was to find out whether and how insulin/IGF axis responds to additional physical activity in the already trained subjects and if so, is the adaptation potentially beneficial from the aspect of metabolic control. Methods: The effect of 4-week intensive training on campus (preparatory training) on the levels of insulin, IGF-1, and IGFBPs during maximal progressive exercise test (MPET) on a treadmill was compared to the results obtained during MPET conducted after a regular training season of a female elite handball team (n = 17, age: 17 +/- 1 years, height: 171 +/- 8 cm, weight: 65 +/- 8 kg, body mass index: 22 +/- 1 kg/m(2) at the beginning of the study; there were no significant changes at the end). Serum samples were obtained from players immediately before the test (basal), at the end of the test after reaching the point of maximal oxygen consumption (VO2max), and after recovery. Results: The concentration of insulin decreased at VO2max, but remained higher in players after preparatory training (12.2 +/- 2.5 mU/L vs. 8.9 +/- 4.4 mU/L, p = 0.049). The level of IGFBP-1 decreased in players at VO2max in either case of training, but it remained much higher in tests performed after the preparatory regime than before (p = 0.029). Concentrations of IGF-1, IGFBP-2, -3, and -4 did not change significantly. Conclusion: The inverse relation between insulin and IGFBP-1 was lost during MPET, as these 2 molecules changed in the same direction. The results obtained suggest less severe stress-induced depression of insulin and IGFBP-1 after preparatory training. But another metabolic mechanism cannot be excluded, and that is potentially impaired insulin sensitivity resulting in higher level of IGFBP-1.",
publisher = "Shanghai Univ Sport, Shanghai",
journal = "Journal of Sport & Health Science",
title = "Preparatory training attenuates drastic response of the insulin-like growth factor binding protein 1 at the point of maximal oxygen consumption in handball players",
pages = "377-372",
number = "3",
volume = "6",
doi = "10.1016/j.jshs.2016.01.014"
}

Nedić, O., Sunderić, M., Miljuš, G., Valdevit, Z., Jakovljević, V., Glibetić, M.,& Vučić, V. M.. (2017). Preparatory training attenuates drastic response of the insulin-like growth factor binding protein 1 at the point of maximal oxygen consumption in handball players. in Journal of Sport & Health Science
Shanghai Univ Sport, Shanghai., 6(3), 372-377.
https://doi.org/10.1016/j.jshs.2016.01.014

Nedić O, Sunderić M, Miljuš G, Valdevit Z, Jakovljević V, Glibetić M, Vučić VM. Preparatory training attenuates drastic response of the insulin-like growth factor binding protein 1 at the point of maximal oxygen consumption in handball players. in Journal of Sport & Health Science. 2017;6(3):372-377.
doi:10.1016/j.jshs.2016.01.014 .

Nedić, Olgica, Sunderić, Miloš, Miljuš, Goran, Valdevit, Zoran, Jakovljević, Vladimir, Glibetić, Marija, Vučić, Vesna M., "Preparatory training attenuates drastic response of the insulin-like growth factor binding protein 1 at the point of maximal oxygen consumption in handball players" in Journal of Sport & Health Science, 6, no. 3 (2017):372-377,
https://doi.org/10.1016/j.jshs.2016.01.014 . .

TY  - JOUR
AU  - Lagundzin, Dragana
AU  - Vučić, Vesna M.
AU  - Glibetić, Marija
AU  - Nedić, Olgica
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/482
AB  - Physical activity is accompanied by the changes in Insulin-like Growth Factor I (IGF-I)/IGF-Binding Protein 1 (IGFBP-1) axis. Inconsistent results concerning IGF-I and IGFBP-1 levels were reported. In this study we have raised some questions on the events that occur at the molecular level of the exercise-related IGFBP-1 changes. We have examined the fragmentation pattern of IGFBP-1, IGFBP-1 protease activity, interaction between IGFBP-1 and alpha2-macroglobulin (alpha 2M), and possible existence of minor structural changes of IGFBP-1 in professional soccer players. Athletes had significantly greater amounts of fragmented IGFBP-1, whereas no difference was found in the amount of intact IGFBP-1 compared with controls. An increased activity of matrix metalloprotease-9 (MMP-9) was detected in athletes, causing IGFBP-1 degradation down to the fragment of 9 kDa as the major one. The amount of alpha 2M, which protects IGFBP-1 from proteolysis, or the amount of IGFBP-1/alpha 2M complexes was unaltered. Finally, we have examined whether IGFBP-1 isolated from soccer players exhibited altered reactivity with several chemical surfaces used in surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS). Different reactivity was detected with anion and cation exchangers, suggesting existence of at least one sequence within IGFBP-1, whose ionization pattern was not equal in athletes and controls. Differences in spectra obtained with ion exchanges may reflect differences in IGFBP-1 phosphorylation. Physiological implications of the events described in this study on the IGF-I availability are, at this time, unknown. It can be hypothesized that IGFBP-1 proteolysis leads to altered distribution of IGF-I among IGFBPs, which may affect the final IGF-associated response.
PB  - Human Kinetics Publ Inc, Champaign
T2  - International Journal of Sport Nutrition & Exercise Metabolism
T1  - Alteration of IGFBP-1 in Soccer Players Due to Intensive Training
EP  - 457
IS  - 5
SP  - 449
VL  - 23
DO  - 10.1123/ijsnem.23.5.449
ER  - 

@article{
author = "Lagundzin, Dragana and Vučić, Vesna M. and Glibetić, Marija and Nedić, Olgica",
year = "2013",
abstract = "Physical activity is accompanied by the changes in Insulin-like Growth Factor I (IGF-I)/IGF-Binding Protein 1 (IGFBP-1) axis. Inconsistent results concerning IGF-I and IGFBP-1 levels were reported. In this study we have raised some questions on the events that occur at the molecular level of the exercise-related IGFBP-1 changes. We have examined the fragmentation pattern of IGFBP-1, IGFBP-1 protease activity, interaction between IGFBP-1 and alpha2-macroglobulin (alpha 2M), and possible existence of minor structural changes of IGFBP-1 in professional soccer players. Athletes had significantly greater amounts of fragmented IGFBP-1, whereas no difference was found in the amount of intact IGFBP-1 compared with controls. An increased activity of matrix metalloprotease-9 (MMP-9) was detected in athletes, causing IGFBP-1 degradation down to the fragment of 9 kDa as the major one. The amount of alpha 2M, which protects IGFBP-1 from proteolysis, or the amount of IGFBP-1/alpha 2M complexes was unaltered. Finally, we have examined whether IGFBP-1 isolated from soccer players exhibited altered reactivity with several chemical surfaces used in surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS). Different reactivity was detected with anion and cation exchangers, suggesting existence of at least one sequence within IGFBP-1, whose ionization pattern was not equal in athletes and controls. Differences in spectra obtained with ion exchanges may reflect differences in IGFBP-1 phosphorylation. Physiological implications of the events described in this study on the IGF-I availability are, at this time, unknown. It can be hypothesized that IGFBP-1 proteolysis leads to altered distribution of IGF-I among IGFBPs, which may affect the final IGF-associated response.",
publisher = "Human Kinetics Publ Inc, Champaign",
journal = "International Journal of Sport Nutrition & Exercise Metabolism",
title = "Alteration of IGFBP-1 in Soccer Players Due to Intensive Training",
pages = "457-449",
number = "5",
volume = "23",
doi = "10.1123/ijsnem.23.5.449"
}

Lagundzin, D., Vučić, V. M., Glibetić, M.,& Nedić, O.. (2013). Alteration of IGFBP-1 in Soccer Players Due to Intensive Training. in International Journal of Sport Nutrition & Exercise Metabolism
Human Kinetics Publ Inc, Champaign., 23(5), 449-457.
https://doi.org/10.1123/ijsnem.23.5.449

Lagundzin D, Vučić VM, Glibetić M, Nedić O. Alteration of IGFBP-1 in Soccer Players Due to Intensive Training. in International Journal of Sport Nutrition & Exercise Metabolism. 2013;23(5):449-457.
doi:10.1123/ijsnem.23.5.449 .

Lagundzin, Dragana, Vučić, Vesna M., Glibetić, Marija, Nedić, Olgica, "Alteration of IGFBP-1 in Soccer Players Due to Intensive Training" in International Journal of Sport Nutrition & Exercise Metabolism, 23, no. 5 (2013):449-457,
https://doi.org/10.1123/ijsnem.23.5.449 . .