TY  - JOUR
AU  - Wu, Ting
AU  - Grootaert, Charlotte
AU  - Pitart, Judit
AU  - Vidović, Nevena Đ.
AU  - Kamiloglu, Senem
AU  - Possemiers, Sam
AU  - Glibetić, Marija
AU  - Smagghe, Guy
AU  - Raes, Katleen
AU  - Van de Wiele, Tom
AU  - Van Camp, John
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/874
AB  - Scope Results To explore the mechanisms behind the health effects of Aronia (Aronia melanocarpa), the microbial community modulating and anti-inflammatory effects of Aronia polyphenols are investigated by combining the similutor of the human intestinal microbial ecosystem (SHIME) with a coculture of intestinal and endothelial cells. Administration of Aronia juice (6.5g L-1) to the SHIME for 2 weeks increases the abundance of firmicutes to 92% in the ascending colon (AC), 85% in the transverse colon (TC), and 82% in the descending colon (DC; p  lt  0.001), proteobacteria (6.7% in AC, p  lt  0.001), and Akkermansia (14% in TC and 18% in DC, p  lt  0.001) and decreases the abundance of Bifidobacterium species, associated with a decrease of acetate and increase of propionate and butyrate, whereas no significant difference is observed upon placebo juice treatment. After addition of the digests to TNF-alpha challenged Caco-2/endothelial cocultures, intercellular adhesion molecule (ICAM)-1, IL-8, and monocyte chemoattractant protein-1 levels are significantly downregulated. Interestingly, Aronia juice treats digests from each colon compartment resulting in a stronger decrease of the ICAM-1 secretion (up to 73%, p  lt  0.001) compared to their corresponding placebo treated digests, thereby pointing to a polyphenol-dependent effect. Conclusions Aronia polyphenols modulate intestinal microbial composition, induce beneficial short chain fatty acid production, and prevent inflammatory stress in endothelial cells. This opens perspectives for the use of Aronia polyphenols as prebiotics in the context of intestinal and cardiovascular health.
PB  - Wiley, Hoboken
T2  - Molecular Nutrition & Food Research
T1  - Aronia (Aronia melanocarpa) Polyphenols Modulate the Microbial Community in a Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and Decrease Secretion of Proinflammatory Markers in a Caco-2/endothelial Cell Coculture Model
IS  - 22
VL  - 62
DO  - 10.1002/mnfr.201800607
ER  - 

@article{
author = "Wu, Ting and Grootaert, Charlotte and Pitart, Judit and Vidović, Nevena Đ. and Kamiloglu, Senem and Possemiers, Sam and Glibetić, Marija and Smagghe, Guy and Raes, Katleen and Van de Wiele, Tom and Van Camp, John",
year = "2018",
abstract = "Scope Results To explore the mechanisms behind the health effects of Aronia (Aronia melanocarpa), the microbial community modulating and anti-inflammatory effects of Aronia polyphenols are investigated by combining the similutor of the human intestinal microbial ecosystem (SHIME) with a coculture of intestinal and endothelial cells. Administration of Aronia juice (6.5g L-1) to the SHIME for 2 weeks increases the abundance of firmicutes to 92% in the ascending colon (AC), 85% in the transverse colon (TC), and 82% in the descending colon (DC; p  lt  0.001), proteobacteria (6.7% in AC, p  lt  0.001), and Akkermansia (14% in TC and 18% in DC, p  lt  0.001) and decreases the abundance of Bifidobacterium species, associated with a decrease of acetate and increase of propionate and butyrate, whereas no significant difference is observed upon placebo juice treatment. After addition of the digests to TNF-alpha challenged Caco-2/endothelial cocultures, intercellular adhesion molecule (ICAM)-1, IL-8, and monocyte chemoattractant protein-1 levels are significantly downregulated. Interestingly, Aronia juice treats digests from each colon compartment resulting in a stronger decrease of the ICAM-1 secretion (up to 73%, p  lt  0.001) compared to their corresponding placebo treated digests, thereby pointing to a polyphenol-dependent effect. Conclusions Aronia polyphenols modulate intestinal microbial composition, induce beneficial short chain fatty acid production, and prevent inflammatory stress in endothelial cells. This opens perspectives for the use of Aronia polyphenols as prebiotics in the context of intestinal and cardiovascular health.",
publisher = "Wiley, Hoboken",
journal = "Molecular Nutrition & Food Research",
title = "Aronia (Aronia melanocarpa) Polyphenols Modulate the Microbial Community in a Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and Decrease Secretion of Proinflammatory Markers in a Caco-2/endothelial Cell Coculture Model",
number = "22",
volume = "62",
doi = "10.1002/mnfr.201800607"
}

Wu, T., Grootaert, C., Pitart, J., Vidović, N. Đ., Kamiloglu, S., Possemiers, S., Glibetić, M., Smagghe, G., Raes, K., Van de Wiele, T.,& Van Camp, J.. (2018). Aronia (Aronia melanocarpa) Polyphenols Modulate the Microbial Community in a Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and Decrease Secretion of Proinflammatory Markers in a Caco-2/endothelial Cell Coculture Model. in Molecular Nutrition & Food Research
Wiley, Hoboken., 62(22).
https://doi.org/10.1002/mnfr.201800607

Wu T, Grootaert C, Pitart J, Vidović NĐ, Kamiloglu S, Possemiers S, Glibetić M, Smagghe G, Raes K, Van de Wiele T, Van Camp J. Aronia (Aronia melanocarpa) Polyphenols Modulate the Microbial Community in a Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and Decrease Secretion of Proinflammatory Markers in a Caco-2/endothelial Cell Coculture Model. in Molecular Nutrition & Food Research. 2018;62(22).
doi:10.1002/mnfr.201800607 .

Wu, Ting, Grootaert, Charlotte, Pitart, Judit, Vidović, Nevena Đ., Kamiloglu, Senem, Possemiers, Sam, Glibetić, Marija, Smagghe, Guy, Raes, Katleen, Van de Wiele, Tom, Van Camp, John, "Aronia (Aronia melanocarpa) Polyphenols Modulate the Microbial Community in a Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and Decrease Secretion of Proinflammatory Markers in a Caco-2/endothelial Cell Coculture Model" in Molecular Nutrition & Food Research, 62, no. 22 (2018),
https://doi.org/10.1002/mnfr.201800607 . .

TY  - JOUR
AU  - Wu, Ting
AU  - Grootaert, Charlotte
AU  - Voorspoels, Stefan
AU  - Jacobs, Griet
AU  - Pitart, Judit
AU  - Kamiloglu, Senem
AU  - Possemiers, Sam
AU  - Heinonen, Marina
AU  - Kardum, Nevena Đ.
AU  - Glibetić, Marija
AU  - Smagghe, Guy
AU  - Raes, Katleen
AU  - Van Camp, John
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/790
AB  - Aronia phenolics are considered to be beneficial for cardiovascular health, but are not always bioavailable. In this study, we evaluated the in vitro bioavailability of Aronia juice phenolics by combination of intestinal digestion models with Caco-2 absorption models. First, intestinal luminal stability and microbial metabolism of Aronia phenolics were investigated using two in vitro digestion models: a short-term batch model and a long term dynamic model (SHIME (R)). Next, diluted digests were directly applied to Caco-2 cells to simulate enterocyte absorption. Three anthocyanins, 5 flavonol glycosides, 5 phenolic acids and epicatechin were quantified at different stages of digestion and absorption. The location of absorption was compound specific, as procyanidins were mainly absorbed in the distal colon while anthocyanins, flavonol glycosides and phenolic acids were absorbed in the proximal intestinal tract. Further, the food matrix present in the colon significantly increased transport without permanent barrier damage.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Aronia (Aronia melanocarpa) phenolics bioavailability in a combined in vitro digestion/Caco-2 cell model is structure and colon region dependent
EP  - 139
SP  - 128
VL  - 38
DO  - 10.1016/j.jff.2017.09.008
ER  - 

@article{
author = "Wu, Ting and Grootaert, Charlotte and Voorspoels, Stefan and Jacobs, Griet and Pitart, Judit and Kamiloglu, Senem and Possemiers, Sam and Heinonen, Marina and Kardum, Nevena Đ. and Glibetić, Marija and Smagghe, Guy and Raes, Katleen and Van Camp, John",
year = "2017",
abstract = "Aronia phenolics are considered to be beneficial for cardiovascular health, but are not always bioavailable. In this study, we evaluated the in vitro bioavailability of Aronia juice phenolics by combination of intestinal digestion models with Caco-2 absorption models. First, intestinal luminal stability and microbial metabolism of Aronia phenolics were investigated using two in vitro digestion models: a short-term batch model and a long term dynamic model (SHIME (R)). Next, diluted digests were directly applied to Caco-2 cells to simulate enterocyte absorption. Three anthocyanins, 5 flavonol glycosides, 5 phenolic acids and epicatechin were quantified at different stages of digestion and absorption. The location of absorption was compound specific, as procyanidins were mainly absorbed in the distal colon while anthocyanins, flavonol glycosides and phenolic acids were absorbed in the proximal intestinal tract. Further, the food matrix present in the colon significantly increased transport without permanent barrier damage.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Aronia (Aronia melanocarpa) phenolics bioavailability in a combined in vitro digestion/Caco-2 cell model is structure and colon region dependent",
pages = "139-128",
volume = "38",
doi = "10.1016/j.jff.2017.09.008"
}

Wu, T., Grootaert, C., Voorspoels, S., Jacobs, G., Pitart, J., Kamiloglu, S., Possemiers, S., Heinonen, M., Kardum, N. Đ., Glibetić, M., Smagghe, G., Raes, K.,& Van Camp, J.. (2017). Aronia (Aronia melanocarpa) phenolics bioavailability in a combined in vitro digestion/Caco-2 cell model is structure and colon region dependent. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 38, 128-139.
https://doi.org/10.1016/j.jff.2017.09.008

Wu T, Grootaert C, Voorspoels S, Jacobs G, Pitart J, Kamiloglu S, Possemiers S, Heinonen M, Kardum NĐ, Glibetić M, Smagghe G, Raes K, Van Camp J. Aronia (Aronia melanocarpa) phenolics bioavailability in a combined in vitro digestion/Caco-2 cell model is structure and colon region dependent. in Journal of Functional Foods. 2017;38:128-139.
doi:10.1016/j.jff.2017.09.008 .

Wu, Ting, Grootaert, Charlotte, Voorspoels, Stefan, Jacobs, Griet, Pitart, Judit, Kamiloglu, Senem, Possemiers, Sam, Heinonen, Marina, Kardum, Nevena Đ., Glibetić, Marija, Smagghe, Guy, Raes, Katleen, Van Camp, John, "Aronia (Aronia melanocarpa) phenolics bioavailability in a combined in vitro digestion/Caco-2 cell model is structure and colon region dependent" in Journal of Functional Foods, 38 (2017):128-139,
https://doi.org/10.1016/j.jff.2017.09.008 . .