TY  - JOUR
AU  - Kuruca, Ljiljana
AU  - Klun, Ivana
AU  - Uzelac, Aleksandra
AU  - Nikolić, Aleksandra
AU  - Bobić, Branko
AU  - Simin, Stanislav
AU  - Lalošević, Vesna
AU  - Lalošević, Dušan
AU  - Đurković-Đaković, Olgica
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/793
AB  - Insufficiently cooked pork is considered as an important source of human infection with Toxoplasma gondii. The aim of our study was to investigate the presence of T. gondii in pigs intended for human consumption from Northern Serbia. Blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. Sera were examined using modified agglutination test (MAT), and diaphragms from seropositive, as well as from some MAT-negative pigs, were bioassayed in mice. In addition, digests were examined for the presence of T. gondii DNA using a real-time polymerase chain reaction (qPCR) which was targeted at the 529 bp repetitive element of the T. gondii genome. The overall seroprevalence of T. gondii in pigs was 17% (31/182), with no difference between pigs from large commercial farms (17.8%) and those raised on smallholdings (16.3%). However, the seroprevalence in farm pigs was largely influenced by the findings on a single farm, where all examined animals tested positive. Parasites and/or parasite DNA were detected in the tissues of 15 of the 45 (25 seropositive and 20 seronegative) animals examined by either direct method. Tissue cysts were isolated in eight bioassays and an additional bioassay was positive by serology; all nine were confirmed positive by qPCR. All positive bioassays originated from seropositive pigs, but no correlation was observed between isolation rate and antibody titer. T. gondii DNA was detected in diaphragm tissues of eight pigs, of which three were seronegative. The results of our study provide further evidence for pork as a source of human T. gondii infection.
PB  - Springer, New York
T2  - Parasitology Research
T1  - Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia
EP  - 3123
IS  - 11
SP  - 3117
VL  - 116
DO  - 10.1007/s00436-017-5623-7
ER  - 

@article{
author = "Kuruca, Ljiljana and Klun, Ivana and Uzelac, Aleksandra and Nikolić, Aleksandra and Bobić, Branko and Simin, Stanislav and Lalošević, Vesna and Lalošević, Dušan and Đurković-Đaković, Olgica",
year = "2017",
abstract = "Insufficiently cooked pork is considered as an important source of human infection with Toxoplasma gondii. The aim of our study was to investigate the presence of T. gondii in pigs intended for human consumption from Northern Serbia. Blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. Sera were examined using modified agglutination test (MAT), and diaphragms from seropositive, as well as from some MAT-negative pigs, were bioassayed in mice. In addition, digests were examined for the presence of T. gondii DNA using a real-time polymerase chain reaction (qPCR) which was targeted at the 529 bp repetitive element of the T. gondii genome. The overall seroprevalence of T. gondii in pigs was 17% (31/182), with no difference between pigs from large commercial farms (17.8%) and those raised on smallholdings (16.3%). However, the seroprevalence in farm pigs was largely influenced by the findings on a single farm, where all examined animals tested positive. Parasites and/or parasite DNA were detected in the tissues of 15 of the 45 (25 seropositive and 20 seronegative) animals examined by either direct method. Tissue cysts were isolated in eight bioassays and an additional bioassay was positive by serology; all nine were confirmed positive by qPCR. All positive bioassays originated from seropositive pigs, but no correlation was observed between isolation rate and antibody titer. T. gondii DNA was detected in diaphragm tissues of eight pigs, of which three were seronegative. The results of our study provide further evidence for pork as a source of human T. gondii infection.",
publisher = "Springer, New York",
journal = "Parasitology Research",
title = "Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia",
pages = "3123-3117",
number = "11",
volume = "116",
doi = "10.1007/s00436-017-5623-7"
}

Kuruca, L., Klun, I., Uzelac, A., Nikolić, A., Bobić, B., Simin, S., Lalošević, V., Lalošević, D.,& Đurković-Đaković, O.. (2017). Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia. in Parasitology Research
Springer, New York., 116(11), 3117-3123.
https://doi.org/10.1007/s00436-017-5623-7

Kuruca L, Klun I, Uzelac A, Nikolić A, Bobić B, Simin S, Lalošević V, Lalošević D, Đurković-Đaković O. Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia. in Parasitology Research. 2017;116(11):3117-3123.
doi:10.1007/s00436-017-5623-7 .

Kuruca, Ljiljana, Klun, Ivana, Uzelac, Aleksandra, Nikolić, Aleksandra, Bobić, Branko, Simin, Stanislav, Lalošević, Vesna, Lalošević, Dušan, Đurković-Đaković, Olgica, "Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia" in Parasitology Research, 116, no. 11 (2017):3117-3123,
https://doi.org/10.1007/s00436-017-5623-7 . .

TY  - JOUR
AU  - Kuruca, Ljiljana
AU  - Klun, Ivana
AU  - Uzelac, Aleksandra
AU  - Nikolić, Aleksandra
AU  - Bobić, Branko
AU  - Simin, Stanislav
AU  - Đurković-Đaković, Olgica
AU  - Lalošević, Vesna
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/713
AB  - Toxoplasma gondii is considered one of the most successful parasites of humans and animals. The ingestion of viable cysts through the consumption of undercooked pork is recognized as a significant route of human infection with T. gondii. The aim of this study was to investigate the presence of viable parasite in tissues of free-range pigs from the Zasavica Special Nature Reserve. All pigs were of the Mangulica breed, raised in a traditional way. The serological screening was performed using a modified agglutination test (MAT). The isolation of viable T. gondii was attempted by a bioassay of pig heart tissue in mice, while the real-time polymerase chain reaction (qPCR) targeted at the 529 bp repetitive element of T. gondii was used to detect parasitic DNA in digested hearts. Specific antibodies were detected in 12 out of 18 pigs examined. The bioassay was performed for five MAT-positive and one MAT-negative pig, and a total of three isolates were obtained. qPCR was performed for all samples, including one MAT-negative sample that was not bioassayed. The presence of T. gondii DNA was confirmed in all hearts with a positive bioassay as well as in one originating from seropositive and one from seronegative pig whose hearts were not bioassayed. The successful isolation of viable cysts, presence of risk factors (such as older age at the time of slaughter) and increased contact with the environment, along with the great appreciation of Serbian consumers towards home-cured Mangulica’s meat, make this breed worthy of consideration as a potentially important reservoir of human infection.
AB  - Toxoplasma gondii predstavlja jednog od najuspešnijih parazita ljudi i životinja. Ingestija vijabilnih cista, putem konzumacije termički nedovoljno obrađenog mesa, smatra se jednim od dominantnih puteva humane infekcije ovim parazitom. Cilj ovog istraživanja bio je da se ispita prisustvo vijabilnih parazita u tkivima slobodno držanih svinja u Specijalnom rezervatu prirode “Zasavica”. Sve ispitane svinje su pripadale rasi mangulica i gajene su na tradicionalan način. Serološka ispitivanja su sprovedena upotrebom testa modifikovane aglutinacije (MAT). Za izolaciju vijabilnih parazita iz tkiva (srca) svinja korišćen je biološki ogled na miševima, dok je reakcija lančane polimeraze u stvarnom vremenu (qPCR), kojom se detektuje 529 bp repetitivni element genoma T. gondii upotrebljena za detekciju parazitske DNK. Specifična antitela su pronađena kod 12 od 18 ispitanih svinja. Biološki ogled je postavljen za 5 MAT-pozitivnih i jednu MAT-negativnu svinju, iz čega su dobijena ukupno tri izolata T. gondii. qPCR je urađen za sve uzorke, uključujući i jedan MAT-negativan uzorak, za koji nije postavljen biološki ogled. DNK T. gondii je dokazana u digestima srca svih svinja iz kojih je parazit uspešno izolovan, kao i kod jedne seropozitivne i jedne seronegativne svinje za čija srca nije postavljen biološki ogled. Izolacija vijabilnih cista, prisustvo faktora rizika kao što je kasnostasnost rase, povećan kontakt sa spoljašnjom sredinom kao i činjenica da su domaći suhomesnati proizvodi od mesa mangulice visoko cenjeni od strane srpskih potrošača, čine ovu rasu svinja potencijalno značajnim rezervoarom T. gondii za ljude.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Detection of viable Toxoplasma gondii in free-range pigs from the Special nature reserve of Zasavica
T1  - Detekcija vijabilnog parazita Toxoplasma gondii kod slobodno držanih svinja iz Specijalnog rezervata prirode Zasavica
EP  - 6
IS  - 3-4
SP  - 1
VL  - 65
DO  - 10.1515/contagri-2016-0010
ER  - 

@article{
author = "Kuruca, Ljiljana and Klun, Ivana and Uzelac, Aleksandra and Nikolić, Aleksandra and Bobić, Branko and Simin, Stanislav and Đurković-Đaković, Olgica and Lalošević, Vesna",
year = "2016",
abstract = "Toxoplasma gondii is considered one of the most successful parasites of humans and animals. The ingestion of viable cysts through the consumption of undercooked pork is recognized as a significant route of human infection with T. gondii. The aim of this study was to investigate the presence of viable parasite in tissues of free-range pigs from the Zasavica Special Nature Reserve. All pigs were of the Mangulica breed, raised in a traditional way. The serological screening was performed using a modified agglutination test (MAT). The isolation of viable T. gondii was attempted by a bioassay of pig heart tissue in mice, while the real-time polymerase chain reaction (qPCR) targeted at the 529 bp repetitive element of T. gondii was used to detect parasitic DNA in digested hearts. Specific antibodies were detected in 12 out of 18 pigs examined. The bioassay was performed for five MAT-positive and one MAT-negative pig, and a total of three isolates were obtained. qPCR was performed for all samples, including one MAT-negative sample that was not bioassayed. The presence of T. gondii DNA was confirmed in all hearts with a positive bioassay as well as in one originating from seropositive and one from seronegative pig whose hearts were not bioassayed. The successful isolation of viable cysts, presence of risk factors (such as older age at the time of slaughter) and increased contact with the environment, along with the great appreciation of Serbian consumers towards home-cured Mangulica’s meat, make this breed worthy of consideration as a potentially important reservoir of human infection., Toxoplasma gondii predstavlja jednog od najuspešnijih parazita ljudi i životinja. Ingestija vijabilnih cista, putem konzumacije termički nedovoljno obrađenog mesa, smatra se jednim od dominantnih puteva humane infekcije ovim parazitom. Cilj ovog istraživanja bio je da se ispita prisustvo vijabilnih parazita u tkivima slobodno držanih svinja u Specijalnom rezervatu prirode “Zasavica”. Sve ispitane svinje su pripadale rasi mangulica i gajene su na tradicionalan način. Serološka ispitivanja su sprovedena upotrebom testa modifikovane aglutinacije (MAT). Za izolaciju vijabilnih parazita iz tkiva (srca) svinja korišćen je biološki ogled na miševima, dok je reakcija lančane polimeraze u stvarnom vremenu (qPCR), kojom se detektuje 529 bp repetitivni element genoma T. gondii upotrebljena za detekciju parazitske DNK. Specifična antitela su pronađena kod 12 od 18 ispitanih svinja. Biološki ogled je postavljen za 5 MAT-pozitivnih i jednu MAT-negativnu svinju, iz čega su dobijena ukupno tri izolata T. gondii. qPCR je urađen za sve uzorke, uključujući i jedan MAT-negativan uzorak, za koji nije postavljen biološki ogled. DNK T. gondii je dokazana u digestima srca svih svinja iz kojih je parazit uspešno izolovan, kao i kod jedne seropozitivne i jedne seronegativne svinje za čija srca nije postavljen biološki ogled. Izolacija vijabilnih cista, prisustvo faktora rizika kao što je kasnostasnost rase, povećan kontakt sa spoljašnjom sredinom kao i činjenica da su domaći suhomesnati proizvodi od mesa mangulice visoko cenjeni od strane srpskih potrošača, čine ovu rasu svinja potencijalno značajnim rezervoarom T. gondii za ljude.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Detection of viable Toxoplasma gondii in free-range pigs from the Special nature reserve of Zasavica, Detekcija vijabilnog parazita Toxoplasma gondii kod slobodno držanih svinja iz Specijalnog rezervata prirode Zasavica",
pages = "6-1",
number = "3-4",
volume = "65",
doi = "10.1515/contagri-2016-0010"
}

Kuruca, L., Klun, I., Uzelac, A., Nikolić, A., Bobić, B., Simin, S., Đurković-Đaković, O.,& Lalošević, V.. (2016). Detection of viable Toxoplasma gondii in free-range pigs from the Special nature reserve of Zasavica. in Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 65(3-4), 1-6.
https://doi.org/10.1515/contagri-2016-0010

Kuruca L, Klun I, Uzelac A, Nikolić A, Bobić B, Simin S, Đurković-Đaković O, Lalošević V. Detection of viable Toxoplasma gondii in free-range pigs from the Special nature reserve of Zasavica. in Savremena poljoprivreda. 2016;65(3-4):1-6.
doi:10.1515/contagri-2016-0010 .

Kuruca, Ljiljana, Klun, Ivana, Uzelac, Aleksandra, Nikolić, Aleksandra, Bobić, Branko, Simin, Stanislav, Đurković-Đaković, Olgica, Lalošević, Vesna, "Detection of viable Toxoplasma gondii in free-range pigs from the Special nature reserve of Zasavica" in Savremena poljoprivreda, 65, no. 3-4 (2016):1-6,
https://doi.org/10.1515/contagri-2016-0010 . .